A5029874401- Immune cells in cancer
- Fatty Acid Research and Health
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Ocular Oncology and Treatments
- Neuroblastoma Research and Treatments
- Sphingolipid Metabolism and Signaling
- Platelet Disorders and Treatments
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Adipose Tissue and Metabolism
- Cardiovascular Effects of Exercise
- Protease and Inhibitor Mechanisms
- Parvovirus B19 Infection Studies
- Cancer-related Molecular Pathways
- Reconstructive Surgery and Microvascular Techniques
- Violence, Education, and Gender Studies
- Antioxidant Activity and Oxidative Stress
- Eicosanoids and Hypertension Pharmacology
- Retinal and Macular Surgery
- Marine Sponges and Natural Products
- Advanced Glycation End Products research
- Exercise and Physiological Responses
Brigham and Women's Hospital
2021-2024
Harvard University
2021-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2023
Academia Nacional de Medicina
2014-2019
National Academy of Medicine
2019
Experimental Medicine and Biology Institute
2019
New York Academy of Medicine
2014
Baylor College of Medicine
1974
We investigated the contribution of human platelets to macrophage effector properties in presence lipopolysaccharide (LPS), as well beneficial effects and time frame for platelet transfusion septic animals. Our results show that sequester both pro-(TNF-α/IL-6) anti-(IL-10) inflammatory cytokines released by monocytes. Low LPS concentrations (0.01 ng/mL) induced M2 polarization decreasing CD64 augmenting CD206 CD163 expression; yet, skewed monocytes toward type 1 (M1) phenotype a...
Abstract Although platelet-rich plasma (PRP) is used as a source of growth factors in regenerative medicine, its effectiveness remains controversial, partially due to the absence PRP preparation protocols based on role platelets. Here, we aimed optimise protocol by analysing angiogenic and properties. Three optimising strategies were evaluated: dilution, 4 °C pre-incubation, cryoprecipitate supplementation. Following coagulation, releasates (PRPr) induce angiogenesis vitro (HMEC-1...
Abstract Dysregulated inflammation‐resolution programs are associated with atherosclerosis progression. Resolvins, in part, mediate programs. Indeed, Resolvin D2 (RvD2) activates GPR18, a G‐protein‐coupled receptor, and limits plaque progression, though the cellular targets of RvD2 remain unknown. Here, we developed humanized GPR18 floxed (“fl/fl”) myeloid (Lysozyme M Cre) knockout (mKO) mouse. We functionally validated this model by assessing efferocytosis bone marrow‐derived macrophages...
Abstract Solid tumors are infiltrated by immune cells where macrophages and senescent T highly represented. Within the tumor microenvironment, a cross-talk between infiltrating may occur conditioning characteristic of in situ response. Our previous work showed that induce senescence cells, which powerful suppressors lympho-proliferation. In this study, we report Tumor-Induced Senescent (TIS)-T also modulate monocyte activation. To gain insight into interaction, CD4+ or CD8+TIS-T control-T...
We have previously demonstrated that acidic preconditioning of human endothelial colony-forming cells (ECFC) increased proliferation, migration, and tubulogenesis in vitro, their regenerative potential a murine model hind limb ischemia without baseline disease. now analyze whether this strategy is also effective under adverse conditions for vasculogenesis, such as the presence ischemia-related toxic molecules or diabetes, one main target diseases cell therapy due to well-known healing...
Background: Platelets mediate angiogenesis through the secretion of several factors, including pro-angiogenic vascular endothelial growth factor (VEGF) and anti-angiogenic endostatin. Although previous findings indicated that these molecules are packed into different alpha-granules selectively released by specific stimulation protease-activated receptor (PAR)-1 or PAR-4, recent evidences against this hypothesis. Objectives: To elucidate controversies about VEGF endostatin release overall...
Purpose: To assess in vitro cytotoxic activity and antiangiogenic effect, ocular systemic disposition, toxicity of digoxin rabbits after intravitreal injection as a potential candidate for retinoblastoma treatment. Methods: A panel two three endothelial cell types were exposed to increasing concentrations conventional (72-hour exposure) metronomic (daily treatment scheme. Cytotoxicity was defined the concentration that killed 50% cells (IC50) assessed with vital dye all types. Induction...
Current treatment of retinoblastoma involves using the maximum dose chemotherapy that induces tumor control and is tolerated by patients. The impact schedule on cytotoxicity has not been studied. Our aim was to gain insight into cytotoxic antiangiogenic effect scheme used in means different vitro models evaluate potential effects multi-drug resistance proteins. Two commercial two patient-derived cell types human vascular endothelial were exposed increasing concentrations melphalan or...
Ceramide 1-phosphate (C1P) is a bioactive sphingolipid highly augmented in damaged tissues. Because of its abilities to stimulate migration murine bone marrow-derived progenitor cells, it has been suggested that C1P might be involved tissue regeneration. In the present study, we aimed investigate whether regulates survival and angiogenic activity human cells with great therapeutic potential regenerative medicine such as endothelial colony-orming (ECFCs). Approach Results: protected ECFC from...
ABSTRACT The arenavirus Junin virus (JUNV) is the etiologic agent of Argentine hemorrhagic fever. We characterized JUNV infection human peripheral blood-derived plasmacytoid dendritic cells (hpDC), demonstrating that hpDC are susceptible to with C#1 strain (attenuated) and even more P (virulent) strain. However, elicited different responses in terms viability, activation, maturation, cytokine expression after both strains.
Abstract Context Activation of brown adipose tissue (BAT) thermogenesis improves insulin sensitivity and is beneficial in obesity. Emerging evidence indicates that BAT activation increases lipid mediators play autocrine endocrine roles to regulate metabolism inflammation. Objective The goal the study was determine relationship between 2 distinct approaches (cold exposure mirabegron treatment) with humans. Methods Healthy female subjects (n = 14) were treated β3-adrenergic receptor agonist...
Monocytes (Mo) are highly plastic myeloid cells that differentiate into macrophages after extravasation, playing a pivotal role in the resolution of inflammation and regeneration injured tissues. Wound-infiltrated monocytes/macrophages more pro-inflammatory at early time points, while showing anti-inflammatory/pro-reparative phenotypes later phases, with dynamic switching depending on wound environment. Chronic wounds often arrested inflammatory phase hampered inflammatory/repair phenotype...
Abstract Introduction/Objective Dysregulated inflammation-resolution programs are associated with atherosclerosis progression. Inflammation-resolution is in part mediated by Resolvins, including Resolvin D2 (RvD2). RvD2, which activates a G-protein coupled receptor (GPCR) called GPR18, limits plaque Cellular targets of RvD2 not known. Approach and Results Here we developed humanized GPR18 floxed (“fl/fl”) myeloid (Lysozyme M Cre) knockout (mKO) mouse. We functionally validated this model...
The mechanisms that link inflammatory responses to cancer development remain a subject of intense investigation, emphasizing the need better understand cellular and molecular pathways create tumor promoting microenvironment. myeloid differentiation primary response protein MyD88 acts as main adaptor molecule for signaling cascades initiated from Toll-like receptors (TLRs) interleukin 1 receptor (IL-1R). has been shown contribute tumorigenesis in many inflammation-associated models. In this...
Es necesario considerar la transicion entre sexto y setimo ano como un proceso de ajuste a una nueva experiencia, el cual debe mantenerse no solo en las semanas del inicio curso lectivo, este es continuo que trabajarlo por tiempo se considere pertinente vez iniciado periodo clases, generando articulacion pedagogica significativa ideas acciones. Para adecuado importante talleres introductorios o espacios desarrollo donde propicien ambiente permita disminuir erradicar temor clase matematica....