A5040861505- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- Virus-based gene therapy research
- Force Microscopy Techniques and Applications
- Virology and Viral Diseases
- RNA modifications and cancer
- RNA Research and Splicing
- Respiratory viral infections research
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- interferon and immune responses
- Methane Hydrates and Related Phenomena
- Renal and related cancers
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
University of Colorado Anschutz Medical Campus
2016-2025
University of Colorado Denver
2011-2015
Tumor cells exhibit an altered metabolism characterized by elevated aerobic glycolysis and lactate secretion which is supported increase in glucose transport consumption. We hypothesized that reducing or eliminating the expression of most prominently expressed transporter(s) would decrease amount available to breast cancer thereby decreasing their metabolic capacity proliferative potential. Of 12 GLUT family transporters mice, GLUT1 was abundantly at RNA level mouse mammary tumors from...
Basal bodies (BBs) are conserved eukaryotic structures that organize cilia. They comprised of nine, cylindrically arranged, triplet microtubules (TMTs) connected to each other by inter-TMT linkages which stabilize the structure. Poc1 is a protein important for BB structural integrity in face ciliary forces transmitted BBs. To understand how confers stability, we identified precise position Tetrahymena and effect loss on binds at TMT inner junctions, stabilizing TMTs directly. From this...
Abstract The cilium is a microtubule-based eukaryotic organelle critical for many cellular functions. Its assembly initiates at basal body and continues as an axoneme that projects out of the cell to form functional cilium. This process tightly regulated. However, our knowledge molecular architecture mechanism limited. By applying cryo-electron tomography, we obtained structures inner junction in three regions from Tetrahymena : proximal, central core body, axoneme. We identified several...
Internal ribosome entry sites (IRESs) are powerful model systems to understand how the translation machinery can be manipulated by structured RNAs and for exploring inherent features of function. The intergenic region (IGR) IRESs from Dicistroviridae family viruses that bind directly initiate co-opting elongation cycle. These require an RNA pseudoknot mimics a codon-anticodon interaction contains conformationally dynamic loop. We explored role this loop found both length sequence essential...
The cilium is a microtubule-based organelle critical for many cellular functions. Its assembly initiates at basal body and continues as an axoneme that projects out of the cell to form functional cilium. This process tightly regulated. However, our knowledge molecular architecture mechanism limited. By applying electron cryo-tomography subtomogram averaging, we obtained subnanometer resolution structures inner junction in three distinct regions cilium: proximal region body, central core...
Basal bodies (BBs) are macromolecular complexes required for the formation and cortical positioning of cilia. Both BB assembly DNA replication tightly coordinated with cell cycle to ensure their accurate segregation propagation daughter cells, but mechanisms ensuring coordination unclear. The Tetrahymena Sas4/CPAP protein is enriched at assembling BBs, localizing core structure base BB-appendage microtubules striated fiber. Sas4 necessary microtubule organization, loss disrupts division...
Basal bodies (BBs) are conserved eukaryotic structures that organize motile and primary cilia. The BB is comprised of nine, cylindrically arranged, triplet microtubules (TMTs) connected to each other by inter-TMT linkages which maintain structure. During ciliary beating, forces transmitted the must be resisted prevent disassembly. Poc1 a protein important for BBs resist forces. To understand how confers stability, we identified precise position binding in