A5056984793- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Epigenetics and DNA Methylation
- Plant Reproductive Biology
- Mitochondrial Function and Pathology
Aix-Marseille Université
2012-2024
Inserm
2012-2024
Centre de Recherche en Cancérologie de Marseille
2018-2024
Centre National de la Recherche Scientifique
2012-2024
AOL (United States)
2023
Memorial Sloan Kettering Cancer Center
2018-2020
Cancer Research Center
2013
Institut Paoli-Calmettes
2013
During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that tethering a PHD-containing protein, Spp1 (a component COMPASS complex), recombinationally cold regions is sufficient induce DSB...
Meiotic recombination shows broad variations across species and along chromosomes is often suppressed at around genomic regions determining sexual compatibility such as mating type loci in fungi. Here, we show that the absence of Spo11-DSBs meiotic on Lakl0C-left, chromosome arm containing sex locus Lachancea kluyveri budding yeast, results from recruitment two axis proteins Red1 Hop1, essential for proper formation. Furthermore, cytological observation spread pachytene reveals Lakl0C-left...
Spp1 is the H3K4me3 reader subunit of Set1 complex (COMPASS/Set1C) that contributes to mechanism by which meiotic DNA break sites are mechanistically selected. We previously proposed a model in interacts with and chromosome axis protein Mer2 leads DSB formation. Here we show spatial interactions occur independently Set1C. exhibits dynamic chromatin binding features during meiosis, many de novo appearing disappearing sites. dynamics depends on its PHD finger Mer2-interacting domain modifiable...
Sex chromosomes in males share only a diminutive homologous segment, the pseudoautosomal region (PAR), wherein meiotic double-strand breaks (DSBs), pairing, and crossing over must occur for correct segregation. How cells ensure PAR recombination is unknown. Here we delineate cis-and trans-acting factors that control ultrastructure make hottest area of DSB formation male mouse genome. Prior to formation, chromosome axes elongate, sister chromatids separate, DSB-promoting hyperaccumulate....
Abstract Meiotic recombination shows broad variations across species and along chromosomes, is often suppressed at around genomic regions determining sexual compatibility such as mating type loci in fungi. Here we show that the absence of Spo11-DSBs meiotic on Lakl0C-left, chromosome arm containing sex locus Lachancea kluyveri budding yeast, results from recruitment two axis proteins Red1 Hop1, essential for proper formation. Furthermore, cytological observation spread pachytene chromosomes...
Double-strand breaks (DSBs) initiate the homologous recombination that is crucial for meiotic chromosome pairing and segregation. Here we unveil mouse ANKRD31 as a lynchpin governing multiple aspects of DSB formation. Spermatocytes lacking have altered locations fail to target DSBs sex chromosomes’ pseudoautosomal regions (PAR). They also delayed/fewer sites but, paradoxically, more DSBs, suggesting dysregulation. Unrepaired failures—stochastic on autosomes, nearly absolute X Y—cause arrest...