A5010254612- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Bacteriophages and microbial interactions
- PARP inhibition in cancer therapy
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Yeasts and Rust Fungi Studies
- Plant Genetic and Mutation Studies
- Genetic factors in colorectal cancer
- Legume Nitrogen Fixing Symbiosis
- Antibiotic Resistance in Bacteria
- Mitochondrial Function and Pathology
- Escherichia coli research studies
- Cancer-related molecular mechanisms research
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Real-time simulation and control systems
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Dendrimers and Hyperbranched Polymers
Università della Svizzera italiana
2019-2024
Institut Curie
2016-2024
Centre National de la Recherche Scientifique
2013-2024
Sorbonne Université
2020-2024
Dynamique de l'information génétique : bases fondamentales et cancer
2024
Université Paris Sciences et Lettres
2019-2023
Marie Curie
2023
Université Toulouse III - Paul Sabatier
2012-2018
Université de Toulouse
2012-2018
Laboratoire de Microbiologie et Génétique Moléculaires
2011-2018
Faithful meiotic chromosome segregation and fertility require recombination between homologous chromosomes rather than the equally available sister chromatid, a bias that in Saccharomyces cerevisiae depends on kinase, Mek1. Mek1 is thought to mediate repair template by specifically suppressing sister-directed repair. Instead, we found when persists closely paired (synapsed) homologues, DNA severely delayed, suggesting suppresses any proximal template. Accordingly, excluded from synapsed...
Article16 November 2018Open Access Transparent process A conserved mechanism drives partition complex assembly on bacterial chromosomes and plasmids Roxanne E Debaugny Laboratoire de Microbiologie et Génétique Moléculaires, Centre Biologie Intégrative (CBI), National la Recherche Scientifique (CNRS), Université Toulouse, UPS, France Search for more papers by this author Aurore Sanchez Jérôme Rech Delphine Labourdette LISBP, CNRS, INRA, INSA, Dorignac Charles Coulomb, CNRS-Université...
Meiotic recombination shows broad variations across species and along chromosomes is often suppressed at around genomic regions determining sexual compatibility such as mating type loci in fungi. Here, we show that the absence of Spo11-DSBs meiotic on Lakl0C-left, chromosome arm containing sex locus Lachancea kluyveri budding yeast, results from recruitment two axis proteins Red1 Hop1, essential for proper formation. Furthermore, cytological observation spread pachytene reveals Lakl0C-left...
Crossovers generated during the repair of programmed meiotic double-strand breaks must be tightly regulated to promote accurate homolog segregation without deleterious outcomes, such as aneuploidy. The Mlh1-Mlh3 (MutLγ) endonuclease complex is critical for crossover resolution, which involves mechanistically unclear interplay between MutLγ and Exo1 polo kinase Cdc5. Using budding yeast gain temporal genetic traction on regulation, we find that constitutively interacts with Exo1. Upon...
Significance During meiosis, programmed chromosome breakage and subsequent double-stranded DNA (dsDNA) break repair help ensure correct segregation promote genetic diversity of the progeny. In budding yeast, which utilizes meiotic recombination pathways conserved in mice humans, majority crossovers are initiated through formation a Holliday junction, requires endonuclease activity Mlh1-Mlh3 mismatch factor to be resolved exclusively into crossover product. Here, we combined structural...
Summary During prophase of the first meiotic division, cells deliberately break their DNA. These DNA breaks are repaired by homologous recombination, which facilitates proper chromosome segregation and enables reciprocal exchange segments between chromosomes, thus promoting genetic diversity in progeny 1 . A successful completion recombination requires nucleolytic processing intermediates. Genetic cellular data implicated a pathway dependent on putative MLH1-MLH3 (MutLγ) nuclease generating...
Abstract The Werner Syndrome helicase, WRN, is a promising therapeutic target in cancers with microsatellite instability (MSI). Long‐term MSI leads to the expansion of TA nucleotide repeats proposed form cruciform DNA structures, which turn cause breaks and cell lethality upon WRN downregulation. Here we employed biochemical assays show that helicase can efficiently directly unfold thereby preventing their cleavage by SLX1‐SLX4 structure‐specific endonuclease. are particularly prone...
ParB proteins are one of the three essential components partition systems that actively segregate bacterial chromosomes and plasmids. In binding to centromere sequences, assembles as nucleoprotein structures called complexes. These assemblies substrates for partitioning process ensures DNA molecules segregated both sides cell. We recently identified sopC nucleotides required homologue plasmid F, SopB. This analysis also suggested a role in an arginine residue, R219, located outside...
The segregation of plasmid F Escherichia coli is highly reliable. Sop partition locus, responsible for this stable maintenance, composed two genes, sopA and sopB a centromere, sopC , consisting 12 direct repeats 43 bp. Each repeat carries 16-bp inverted motif to which SopB binds form nucleoprotein assembly called the complex. A database search sequences closely related revealed unexpected features that appeared conserved. We have investigated requirements specific SopB– interactions using...
The remodeling of stalled replication forks to form four-way DNA junctions is an important component the stress response. Nascent at regressed arms these reversed protected by RAD51 and tumor suppressors BRCA1/2, when this function compromised, undergo pathological MRE11-dependent degradation, leading chromosomal instability. However, mechanisms regulating MRE11 functions are currently unclear. Here, we identify MRE11-binding protein MRNIP as a novel fork protection factor that directly...
<title>Abstract</title> DNA double-strand break (DSB) repair by homologous recombination (HR) is initiated end resection, a process involving the controlled degradation of 5'-terminated strands at DSB sites 1,2. The breast cancer suppressor BRCA1-BARD1 not only promotes resection and HR, but it also protects upon replication stress 1,3-9. While counteracts anti-resection pro-non-homologous end-joining factor 53BP1, its direct role in has been unclear, particularly due to complex phenotypes...
Abstract Crossovers generated during the repair of programmed double-strand breaks (DSBs) homologous recombination are essential for fertility to allow accurate homolog segregation first meiotic division. Most crossovers arise through cleavage intermediates by Mlh1-Mlh3 (MutLγ) endonuclease and an elusive non-catalytic function Exo1, require Polo kinase Cdc5. Here we show in budding yeast that MutLγ forms a constitutive complex with cells transiently contacts Msh4-Msh5 (MutSγ) heterodimer,...
Abstract Meiotic recombination shows broad variations across species and along chromosomes, is often suppressed at around genomic regions determining sexual compatibility such as mating type loci in fungi. Here we show that the absence of Spo11-DSBs meiotic on Lakl0C-left, chromosome arm containing sex locus Lachancea kluyveri budding yeast, results from recruitment two axis proteins Red1 Hop1, essential for proper formation. Furthermore, cytological observation spread pachytene chromosomes...
Summary Chromosome and plasmid segregation in bacteria are mostly driven by ParABS systems. These DNA partitioning machineries rely on large nucleoprotein complexes assembled centromere sites ( parS ). However, the mechanism of how a few -bound ParB proteins nucleate formation highly concentrated clusters remains unclear despite several proposed physico-mathematical models. We discriminated between these different models varying some key parameters vivo using F partition system. found that...
RAD51 functions in DNA double-strand break repair by homologous recombination, and a yet undefined mechanism the metabolism of challenged replication forks. Here we show that directly specifically promotes strand annealing branch migration activities SMARCAL1 ZRANB3 but not HLTF, stimulating thus fork reversal. We also find paralog complex, RAD51B-RAD51C-RAD51D-XRCC2 (BCDX2), additionally stimulates remodeling. binding is required, interplay RAD51, paralogs remodelers involves direct...
Abstract SMARCAL1, ZRANB3 and HLTF are all required for the remodeling of replication forks upon stress. Using reconstituted reactions, we show that motor proteins have unequal biochemical capacities, explaining why they non-redundant functions. Whereas SMARCAL1 uniquely anneals RPA-coated ssDNA, suggesting an initial function in fork reversal, it becomes comparatively inefficient subsequent branch migration. We also low concentrations RAD51 paralog complex, RAD51B-RAD51C-RAD51D-XRCC2...