A5012493693- Ubiquitin and proteasome pathways
- Civil and Geotechnical Engineering Research
- Geomechanics and Mining Engineering
- Structural Behavior of Reinforced Concrete
- Crystallization and Solubility Studies
- Innovative concrete reinforcement materials
- X-ray Diffraction in Crystallography
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Metal-Organic Frameworks: Synthesis and Applications
- Nuclear Structure and Function
- Structural Load-Bearing Analysis
- Grouting, Rheology, and Soil Mechanics
- Advanced Photocatalysis Techniques
- RNA modifications and cancer
- Nematode management and characterization studies
- Mitochondrial Function and Pathology
- BIM and Construction Integration
- Circular RNAs in diseases
- TiO2 Photocatalysis and Solar Cells
- Structural Engineering and Vibration Analysis
- Protein Degradation and Inhibitors
- Pulsed Power Technology Applications
- ATP Synthase and ATPases Research
- Structural Response to Dynamic Loads
Anhui Agricultural University
2013-2024
Lishui Maternity and Child Health Care Hospital
2023-2024
The Fifth People's Hospital of Anyang
2022
Wayne State University
2011-2021
Buffalo State University
2021
University at Buffalo, State University of New York
2021
Central People's Hospital of Zhanjiang
2021
State Key Laboratory of Clean Energy Utilization
2020
Zhejiang University
2020
Affiliated Hospital of Nantong University
2019
Sumoylation is essential for progression through mitosis, but the specific protein targets and functions remain poorly understood. In this study, we used chromosome spreads to more precisely define localization of SUMO-2/3 (small ubiquitin-related modifier) inner centromere scaffold mitotic chromosomes. We also developed methods immunopurify proteins modified by endogenous, untagged from Using these methods, identified 149 chromosome-associated substrates nLC-ESI-MS/MS. Approximately...
SUMOylation is an essential posttranslational modification and regulates many cellular processes. Dysregulation of plays a critical role in metastasis, yet how its perturbation affects this lethal process cancer not well understood. We found that SUMO-2/3 greatly up-regulated metastatic breast cells compared with nonmetastatic control cells. To identify proteins differentially modified by between cells, we established method which endogenous conjugates are labeled stable isotope labeling...
Annulate lamellae are cytoplasmic organelles containing stacked sheets of membranes embedded with pore complexes. These complexes at annulate morphologically similar to nuclear the envelope. Although has been observed in nearly all types cells, their biological functions still largely unknown. Here we show that SUMO1-modification Ran GTPase-activating protein RanGAP1 not only target its known sites but also through interactions Ran-binding RanBP2 and SUMO-conjugating enzyme Ubc9 mammalian...
Ubiquitin (Ub) and the ubiquitin-like proteins (Ubls) comprise a remarkable assortment of polypeptides that are covalently conjugated to target (or other biomolecules) modulate their intracellular localization, half-life, and/or activity. Identification Ub/Ubl conjugation sites on protein interest can thus be extremely important for understanding how it is regulated. While MS has become powerful tool study many classes PTMs, identification presents number unique challenges. Here, we present...
Abstract Background Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by the progressive destruction of insulin‐production pancreatic β cells. Recently, microRNAs (miRNAs) have emerged as important regulators in T1D. The present study aimed to determine miR‐885‐3p expression T1D patients and examine effects on inflammatory response human monocytes. Methods Relevant gene levels were determined quantitative polymerase chain reaction; western blotting enzyme‐linked...
SUMO modification is required for the kinetochore localization of kinesin-like motor protein CENP-E, which subsequently mediates alignment chromosomes to spindle equator during mitosis. However, underlying mechanisms by sumoylation regulates CENP-E are still unclear. In this study, we first elucidate that Nuf2 not only but also preferentially modified poly-SUMO-2/3 chains. addition, significantly upregulated mitosis, temporally correlated We further show mitotic defects in and chromosome...
Microalgae with a high growth rate and carbon fixation present promising potential to produce diverse renewable energy products reduce greenhouse gas emissions. However, some microalgal species may have limited light-use efficiency specific rate. To alleviate these issues, Nannochloropsis oceanica was subjected 137Cs –γ radiation obtain the desired mutant enhanced increased biomass productivity. The N. ZJU700 showed 26.7% increase in productivity after nuclear irradiation at 700GY dosage. It...