A5064665613- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Protein Degradation and Inhibitors
- Vector-Borne Animal Diseases
- interferon and immune responses
- DNA Repair Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Histone Deacetylase Inhibitors Research
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- Sexual Differentiation and Disorders
- HIV Research and Treatment
- Acute Lymphoblastic Leukemia research
- Chromosomal and Genetic Variations
- Immune Response and Inflammation
- Nuclear Structure and Function
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- Cancer-related Molecular Pathways
- NF-κB Signaling Pathways
Inserm
2003-2020
Institut Pasteur
2003-2020
La Ligue Contre le Cancer
2013-2020
Organisation Nucléaire et Oncogenèse
2009-2018
University of Copenhagen
2004
University of Perugia
1996
The University of Texas Southwestern Medical Center
1992-1995
Boston University
1989
The ubiquitin-related SUMO-1 molecule has been shown recently to modify covalently a number of cellular proteins including IκBα. modification was found antagonize IκBα ubiquitination and protect it from degradation. Here we identify the transcription factors c-Jun p53, two well known targets ubiquitin, as new substrates for both in vitro vivo. In contrast preferentially single lysine residue (Lys-229), abrogation does not compromise its ubiquitination. Activation Jun NH2-terminal kinases,...
The PML/SP100 nuclear bodies (NBs) were first described as discrete subnuclear structures containing the SP100 protein. Subsequently, they shown to contain PML protein which is part of oncogenic PML-RARα hybrid produced by t(15;17) chromosomal translocation characteristic acute promyelocytic leukemia. Yet, physiological role these remains unknown. Here, we show that binds members heterochromatin 1 (HP1) families non-histone proteins. Further, demonstrate a naturally occurring splice variant...
The PML protein was first identified as part of a fusion product with the retinoic acid receptor alpha (RAR alpha), resulting from t(15;17) chromosomal translocation associated acute promyelocytic leukemia (APL). It has been previously demonstrated that PML, which is tightly bound to nuclear matrix, concentrates in discrete subnuclear compartments are disorganized APL cells due expression PML-RAR hybrid. Here we report adenovirus infection causes drastic redistribution spherical bodies into...
One in seven couples worldwide are infertile, and male factor infertility accounts for approximately 30%–50% of these cases. Although many genes known to be essential gametogenesis, there surprisingly few monogenic mutations that have been conclusively demonstrated cause human spermatogenic failure. A nuclear receptor, NR5A1 (also called steroidogenic 1), is a key transcriptional regulator involved the hypothalamic-pituitary-steroidogenic axis, it expressed tissue developing adult gonad....
Despite numerous studies on specific sumoylated transcriptional regulators, the global role of SUMO chromatin in relation to transcription regulation remains largely unknown. Here, we determined genome-wide localization SUMO1 and SUMO2/3, as well UBC9 (encoded by UBE2I ) PIASY PIAS4 ), two markers for active sumoylation, along with Pol II histone marks proliferating versus senescent human fibroblasts together gene expression profiling. We found that, whereas alone is widely distributed over...
AbstractTumor suppressor HIC1 (hypermethylated in cancer 1) is a gene that essential for mammalian development, epigenetically silenced many human tumors, and involved complex pathway regulating P53 tumor suppression activity. encodes sequence-specific transcriptional repressor containing five Krüppel-like C2H2 zinc fingers an N-terminal BTB/POZ repression domain. Here, we show endogenous SUMOylated vivo on phylogenetically conserved lysine, K314, located the central region which second...
Arkadia is a RING domain E3 ubiquitin ligase that activates the transforming growth factor β (TGF-β) pathway by inducing degradation of inhibitor SnoN/Ski. Here we show contains three successive SUMO-interacting motifs (SIMs) mediate noncovalent interaction with poly-SUMO2. We identify third SIM (VVDL) to be most relevant one in this interaction. Furthermore, provide evidence can function as SUMO-targeted (STUBL) ubiquitinating SUMO chains. While SIMs are not essential for SnoN/Ski response...
The SP100 protein, together with PML, represents a major constituent of the PML-SP100 nuclear bodies (NBs). function these ubiquitous subnuclear structures, whose integrity is compromised in pathological situations such as acute promyelocytic leukemia (APL) or DNA virus infection, remains poorly understood. There little evidence for occurrence actual physiological processes within NBs. two NB proteins PML and are covalently modified by ubiquitin-related SUMO-1 modifier, recent work indicates...
Gene expression from the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) is mediated by three cis-acting regulatory elements known as 21-bp repeats and transactivator protein Tax. The can be subdivided into motifs A, B, C, each of which important for maximal gene in response to B motif contains nucleotide sequences a cyclic AMP element (CRE) or tax-response binds members ATF/CREB family transcription factors. Though mutations this HTLV-I LTR eliminate tax activation,...
Gene silencing via heterochromatin formation plays a major role in cell differentiation and maintenance of homeostasis. Here we report the identification characterization novel heterochromatinization factor vertebrates, bromo adjacent homology domain-containing protein 1 (BAHD1). This nuclear interacts with HP1, MBD1, HDAC5, several transcription factors. Through electron immunofluorescence microscopy studies, show that BAHD1 overexpression directs HP1 to specific sites promotes large...
The sheltering of chromosome ends from illegitimate DNA repair reactions and telomere length homeostasis are critical for preserving genomic integrity. Growing evidence implicates covalent protein modification by SUMO (small ubiquitin-like modifier) (sumoylation) in the regulation numerous transactions, including transcription, as well heterochromatin formation maintenance. We have recently shown that fission yeast Pli1p is a E3 ligase pli1 mutants, which impaired global sumoylation, viable,...
Gene expression of human immunodeficiency virus (HIV) is modulated by both cellular transcription factors, which bind to cis-acting regulatory elements in the HIV-1 long terminal repeat (LTR) and viral transactivator, tat. The enhancer element LTR extends from -103 -82 critical for gene expression. This region contains two identical 10-bp direct repeats serve as binding sites members NF-kappa B family factors. However, several other including a group zinc finger DNA-binding proteins, also...