A5100452576- Telomeres, Telomerase, and Senescence
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Pain Mechanisms and Treatments
- Renal and related cancers
- Biochemical effects in animals
- Diet and metabolism studies
- Transcranial Magnetic Stimulation Studies
- GDF15 and Related Biomarkers
- Pain Management and Treatment
- MicroRNA in disease regulation
- Genetics, Aging, and Longevity in Model Organisms
- Cancer Cells and Metastasis
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Ferroptosis and cancer prognosis
- Fungal Infections and Studies
- RNA Interference and Gene Delivery
- Antifungal resistance and susceptibility
- Ubiquitin and proteasome pathways
- Dietary Effects on Health
- Adipokines, Inflammation, and Metabolic Diseases
- Sirtuins and Resveratrol in Medicine
- Tryptophan and brain disorders
Shanghai Institute of Materia Medica
2024-2025
University of Chinese Academy of Sciences
2024-2025
Institut Pasteur
2015-2024
Centre National de la Recherche Scientifique
2018-2024
Université Paris Cité
2023-2024
Biologie du Développement et Cellules Souches
2016-2023
China Institute of Sport Science
2018-2023
Xinxiang Medical University
2022-2023
Dalian Polytechnic University
2023
Heidelberg University
2021-2022
The generation of induced pluripotent stem cells (iPSC) from adult somatic is one the most remarkable discoveries in recent decades. However, several works have reported evidence genomic instability iPSC, raising concerns on their biomedical use. reasons behind observed iPSC remain mostly unknown. Here we show that, similar to phenomenon oncogene-induced replication stress, expression reprogramming factors induces stress. Increasing levels checkpoint kinase 1 (CHK1) reduces...
Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy lactation, involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium as well surrounding stroma. Three-dimensional (3D) organoid culture has become an important tool biology enabled invaluable discoveries on pubertal branching morphogenesis breast cancer. However, a suitable 3D model recapitulating key...
The primary motor cortex (M1) is involved in the control of voluntary movements and extensively mapped this capacity. Although M1 implicated modulation pain, underlying circuitry causal underpinnings remain elusive. We unexpectedly unraveled a connection from to nucleus accumbens reward through layer 6-mediodorsal thalamus pathway, which specifically suppresses negative emotional valence associated coping behaviors neuropathic pain. By contrast, 5 neurons connect with specific cell...
Cognitive decline and mood disorders increase in frequency with age. Many efforts are focused on the identification of molecules pathways to treat these conditions. Here, we demonstrate that systemic administration growth differentiation factor 11 (GDF11) aged mice improves memory alleviates senescence depression-like symptoms a neurogenesis-independent manner. Mechanistically, GDF11 acts directly hippocampal neurons enhance neuronal activity via stimulation autophagy. Transcriptomic...
Ku70 forms a heterodimer with Ku80, called Ku, that is critical for repairing DNA double-stand breaks by nonhomologous end joining and maintaining telomeres. Mice either gene mutated exhibit similar phenotypes include increased sensitivity to ionizing radiation severe combined immunodeficiency. However, there are also differences in the reported phenotypes. For example, only mutants high incidence of thymic lymphomas while Ku80 early aging very low cancer levels. There two explanations these...
Aging is a negative regulator of general homeostasis, tissue function, and regeneration. Changes in organismal energy levels physiology, through systemic manipulations such as calorie restriction young blood infusion, can regenerate activity increase lifespan aged mice. However, whether these two could be linked has never been investigated. Here, we report that GDF11 triggers restriction-like phenotype without affecting appetite or GDF15 the blood, restores insulin/IGF-1 signaling pathway,...
Background The NAD-dependent deacetylase SIRT1 is a nutrient-sensitive coordinator of stress-tolerance, multiple homeostatic processes and healthspan, while p53 stress-responsive transcription factor our paramount tumour suppressor. Thus, SIRT1-mediated inhibition has been identified as key node in the common biology cancer, metabolism, development ageing. However, precisely how integrates such diverse remains to be elucidated. Methodology/Principal Findings Here we report that alternatively...
Cellular senescence is an irreversible growth arrest with a dynamic secretome, termed the senescence-associated secretory phenotype (SASP). Senescence cell-intrinsic barrier for reprogramming, whereas SASP facilitates cell fate conversion in non-senescent cells. However, mechanisms by which reprogramming-induced regulates plasticity are not well understood. Here, we investigate how heterogeneity of paracrine impacts reprogramming. We show that promotes vitro reprogramming stress-dependent...
Abstract Transcranial, minimally-invasive stimulation of the primary motor cortex (M1) has recently emerged to show promise in treating clinically refractory neuropathic pain. However, there is a major need for improving efficacy, reducing variability and understanding mechanisms. Rodent models hold helping overcome these obstacles. still remains divide between clinical preclinical studies with respect programs, analysis pain as multidimensional sensory-affective-motivational state lack...
Osteoarthritic pain has a strong impact on patients’ quality of life. Understanding the pathogenic mechanisms underlying osteoarthritic will likely lead to development more effective treatments. In present study model rats, we observed reduction M-current density and remarkable decrease in levels KCNQ2 KCNQ3 proteins mRNAs dorsal root ganglia (DRG) neurons, which were associated with hyperalgesic behaviors. The activation KCNQ/M channels flupirtine significantly increased mechanical...
Induction of pluripotency in somatic cells with defined genetic factors has been successfully used to investigate the mechanisms disease initiation and progression. Cellular reprogramming oncogenic transformation share common features; both involve undergoing a dramatic change cell identity, immortalization is key step for cancer progression that enhances reprogramming. However, there are very few examples complete successful tumor cells. Here we address effect expressing an active oncogene,...
Purpose: Thermogenesis function has made brown/beige adipocyte an attractive target for obesity. Human brown adipose tissue activity is impaired in obesity vivo. The present study aims to compare the differences beige differentiation potential of subcutaneous derived from normal weight and obese Chinese individuals vitro. Methods: Adipose-derived stem cells (ADSCs) isolated fat tissues (NW) (OB) groups were induced differentiate into mature with white (WA)- (BA)-induction treatment....
Neurostimulation-based therapeutic approaches are emerging as alternatives to pharmacological drugs, but need further development optimize efficacy and reduce variability. Despite its key relevance pain, the insular cortex has not been explored in cortical neurostimulation approaches. Here, we developed an approach perform repetitive transcranial direct current stimulation of posterior insula (PI tDCS) studied impact on sensory aversive components neuropathic pain pain-related anxiety...
We present a protocol to evaluate the impact of senescence secretome on reprogramming pluripotency using both cellular and mouse models. First, we describe in vitro procedure conditioned medium derived from senescent cells. Next, explore vivo reprogramming, detail steps identify reprogrammed cells skeletal muscle, followed by semi-automatic quantification. This can be used study effect paracrine plasticity. For complete details use execution this protocol, please refer von Joest et al. (2022).1