Novel, «green» and simple visible spectrophotometric procedures for the determination of six dihydropyridines CCBs (amlodipine besylate (AML), lacidipine (LAC), levamlodipine (LAML), nifedipine (NIF), nimodipine (NIM) nitrendipine (NIT)) through derivatization with sulfophthalein dye bromophenol blue (BPB) have been developed. The optimal parameters analysis via complex formation using BPB were as follows: detection wavelength—596 nm, reaction time—5 min, ratio reacting components—1:1,...

Abstract A new difference spectrophotometric method for the determination of prothipendyl hydrochloride in commercial pharmaceutical preparations has been proposed. The includes oxidation an aliquot drug solution with potassium caroate to form corresponding sulfoxide ( ε = (5.83 ± 0.07) × 10 3 L/mol cm) and subsequent measurement absorbance at 340 nm compared that unoxidized equal concentration. graph Beer’s law shows Δ values measured wavelength are proportional concentration range 1.5–70...

The aim of the work was to develop a spectrophotometric method for determination rosuvastatin in tablets based on reaction with BPB compliance principles «green» chemistry.
 Material and methods. Analytical equipment: two-beam UV-visible spectrophotometer Shimadzu model -UV 1800 (Japan), software UV-Probe 2.62, electronic laboratory balance RAD WAG AS 200/C. following APIs, dosage forms, reagents solvents were used work: pharmacopoeial standard sample (CRS) calcium (Sigma-Aldrich, (≥ 98...

Abstract Metopimazine (MPZ) is a dopamine antagonist from the phenothiazine class of drugs. It has antiemetic properties and used to treat nausea vomiting, including those associated with cancer chemotherapy. We have proposed new method for quantitative determination by spectrophotometry. The drug determined using difference spectrophotometric technique based on absorbance its previously prepared sulfoxide derivative ( λ max = 355 nm, ε 4.5 × 10 3 L/mol cm) relative solution non-derivatised...

ABSTRACT Simultaneous determination of a tandem drugs like perindopril and rosuvastatin with differences in water organic solvent solubilities, hydrophobicity, polarities, UV‐absorbing possibilities between them necessitates careful selection chromatographic conditions. The four specified different bonded reversed‐phase columns were tested running mobile phases containing various composition contents concepts; the concept using diluted trifluoroacetic acid phase acetonitrile enabled...

The aim of the work was to develop a simple, eco-friendly, quick, affordable and alternative spectrophotometric procedure that uses azodye methyl red (MR) for determination lercanidipine in its dosage form considering “green” chemistry principles. Materials methods. Analytical equipment: Shimadzu UV-1800 double beam UV-visible spectrophotometer (Japan) with included UV-Probe 2.70 software, RAD WAG AS 200/C precise analytical balance (Poland), Elmasonic EASY 40H ultrasonic bath. Lercanidipine...

The aim of the work was to develop and validate a spectrophotometric method for determining metoprolol tartrate in tablets based on reaction with bromocresol green (BCG). Material methods. Analytical equipment: two-beam UV-visible spectrophotometer Shimadzu model -UV 1800 (Japan), software UV-Probe 2.62, laboratory electronic balance RAD WAG AS 200/C, pH-meter И-160МИ. following APIs, dosage forms, reagents solvents were used work: pharmacopoeial standard sample (CRS) (Sigma-Aldrich, (≥ 98...

The aim of the study was to research qualitative composition and investigate quantitative content some carboxylic acids in herbal mixtures with established hypoglycemic, hypolipidemic antioxidant activity previous studies vivo.
 Materials methods. Studies acid were performed by HPLC analysis using Agilent Technologies 1200 liquid chromatograph (USA). Identification standard solutions compounds (tartaric, pyruvic, isocitric, citric, succinic fumaric acids).
 Conclusions. five...

Two simple, rapid, green non extractive spectrophotometric methods are described for the estimation of valsartan in tablet dosage form. The determination is based on ion-pair formation using dyes, bromophenol blue (BPB) and methyl red (MR). Valsartan forms complex selectively with as indicated by a coloured BPB at pH 5.5 λmax 424 nm MR 4.3 494 nm. For both methods, optimal conditions were established. linear relationship was found between absorbance concentration drug range 8–24 µg/mL 4–20...

The aim of the work was to develop two simple, rapid, economic, alternative spectrophotometric methods for determination ramipril in tablets based on reaction with sulfonephthalein dyes (bromphenol blue (BPB) and cresol red (CR)). Materials methods. Analytical instrumentation: Shimadzu UV-1800 double beam UV-VIS spectrophotometer (Japan) attached UV-Probe ver. 2.62 software, RAD WAG AS 200/C precise analytical balance (Poland). Ramipril (purity ≥98 % (HPLC)) purchased from AARTI Industries...

The aim of the work was to develop a «green» and extraction-free spectrophotometric procedure for assay rosuvastatin in tablets. present describes three new procedures (A, B C) that can be utilized routine quality control laboratories. Materials methods. Analytical instrumentation: Shimadzu UV-1800 double beam UV-vis spectrophotometer (Japan) with attached UV-Probe ver. 2.62 software, RAD WAG AS 200/C precise analytical balance (Poland). Rosuvastatin calcium (purity ≥98 % (HPLC)) bought from...

Nowadays, there is a need to expand the bank of spectrophotometric methods for determination perindopril in dosage forms purposes routine pharmaceutical analysis, which would be simple, express, «green» and inexpensive. In present work, tablets was quantified via direct «green», non-extracting approach based on formation ion-pair complexes with sulphophtalein dyes. The absorbances colored reaction products were registered at 405 nm (bromocresol green, BCG), 397 purple, BCP, bromothymol blue,...

Одним із напрямів професійної діяльності для здобувачів вищої освіти ОПП «Фармація» у Тернопільському національному медичному університеті iменi I. Я. Горбачевського Міністерства охорони здоровʼя України після закінчення навчання є робота в лабораторіях вивчення фармакокінетики та бiоеквiвалентностi ЛЗ. Нова вибіркова дисципліна «Методологія біоаналітичних досліджень» впроваджена навчальний процес 2022–2023 н. р. V курсу фармацевтичного факультету очної заочної форм навчання. Методологія...

A new difference spectrophotometric method for the analysis of prothipendyl hydrochloride in commercial pharmaceutical preparations has been proposed. The includes oxidation an aliquot drug solution with potassium caroate to form corresponding sulfoxide (ε278 = (13.69 ±0.01)×103 L mol/cm) and subsequent measurement optical density at 278 nm compared that unoxidized equal concentration. graph Beer's law shows ΔA values measured wavelength are proportional concentration range 3.2-60 µg/mL....

The aim of the work was to develop a simple, economic, fast, reliable, and eco-friendly spectrophotometric method for determination atorvastatin in tablets based on reaction with bromothymol blue (BTB).
 Material methods. A double–beam Shimadzu UV-Visible spectrophotometer, spectral bandwidth 1 nm wavelength accuracy ±0.5 nm, Model –UV 1800 (Japan), Software UV-Probe 2.62 used measure absorbance resulting solution. Pharmacopeial standard sample calcium BTB were provided by Sigma-Aldrich...

A simple, rapid, and inexpensive spectrophotometric method for the determination of atorvastatin calcium in tablets has been developed. It requires only one reagent (BPB) suitable routine pharmaceutical analysis with less sophisticated equipment. The procedure is based on its interaction bromophenol blue medium organic solvent. As a result reaction, intensity band doubly ionized form BPB maximum at 595 nm increases. analytical signal observed solution methanol, chloroform, or acetonitrile,...

The aim of the work was to develop a simple, economical and eco-friendly spectrophotometric method for determining metoprolol tartrate in tablets based on reaction with bromophenol blue (BPB).
 Material methods: A double–beam Shimadzu UV-Visible spectrophotometer, spectral bandwidth 1 nm wavelength accuracy ±0.5 nm, Model –UV 1800 (Japan), Software UV-Probe 2.62, pair cm matched quartz cells, used measure absorbance resulting solution. All chemicals were analytical reagent grade....

A simple and express method for the quantitative determination of zopiclone in model solutions substance "Zopiclone" tablets, 7.5 mg, by kinetic-spectrophotometric according to 3,3',5,5'-tetramethylbenzidine oxidation has been developed. It is based on system two coupled reaction: 4-methyl-1-piperazineperoxycarboxylic acid generated perhydrolysis reacts with excess hydrogen peroxide weak alkaline medium formation coloured 3,3',5,5'-tetramethyldiphenylquinone diimine (λmax = 420 nm, рН 8.4)....

Two simple, rapid and green spectrophotometric methods are described for the determination of lisinopril medicines. The is based on reaction primary amino group with ninhydrin in aqueous medium (Method I) carboxylic copper (II) sulfate II). For both methods, optimal conditions were established. linear relationship was found between absorbance at λmax concentration drug range 40–60 µg/mL 0.592–2.072 mg/mL Regression analysis Beer’s law plot 400 nm yielded regression equation, y = 7.4929x –...

A novel selective, simple, sensitive, and cost effective kinetic spectrophotometric initial rate method for the determination of suxamethonium chloride has been developed.It is based on indicator reaction catalytic 3,3',5,5'-tetramethylbenzidine oxidation.The analytical performance was validated statistically with respect to limit detection (LOD) quantitation (LOQ), accuracy, precision, linearity.

The aim of the work was to create an approach for development HPLC methods determination meldonium in dosage forms with usage salts chaotropic anions mobile phases.
 Material and methods. Analytical equipment: Shimadzu UPLC system LC-40 PDA; Nexera-i LC-2040C 3D-Plus, controlled by software Lab Solution version 5.97, electronic laboratory balance RAD WAG AS 200/C, pH-meter I-160MI. Meldonium dihydrate (purity 99.3 %) purchased from Sigma-Aldrich (Switzerland), Vasopro capsules 500 mg...

A new difference spectrophotometric method for the analysis of alimemazine in commercial formulations has been proposed. The is based on oxidizing drug with potassium caroate formation corresponding sulfoxide (ε = (4.7 ± 0.06) · 103 L mol-1 cm-1) and measuring absorbance solution at 342 nm using an unoxidized same concentration as blank. graph Beer's law tartrate showed that ΔA values measured wavelength maximum are directly proportional to oxidized solutions range 2-30 μg/mL....

The aim of the work was to develop and validate a spectrophotometric method for determination rosuvastatin in tablets based on reaction with BPB. Material methods. Analytical equipment: two-beam UV-visible spectrophotometer Shimadzu model -UV 1800 (Japan), software UV-Probe 2.62, laboratory electronic balance RAD WAG AS 200/C. following APIs, dosage forms, reagents solvents were used work: pharmacopoeial standard sample (CRS) calcium (Sigma-Aldrich, (≥ 98%, HPLC)), BPB "Rosuvastatin" 10 mg,...