A5047380243- SARS-CoV-2 detection and testing
- Biochemical and Structural Characterization
- Neurological diseases and metabolism
- Advanced Proteomics Techniques and Applications
- Metabolism and Genetic Disorders
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Insect Resistance and Genetics
- Signaling Pathways in Disease
- Mitochondrial Function and Pathology
- Sirtuins and Resveratrol in Medicine
- RNA modifications and cancer
- Insect and Arachnid Ecology and Behavior
- PARP inhibition in cancer therapy
- Genomics and Chromatin Dynamics
- Porphyrin Metabolism and Disorders
- Neurobiology and Insect Physiology Research
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- COVID-19 diagnosis using AI
- Epigenetics and DNA Methylation
- COVID-19 epidemiological studies
- Biochemical and Molecular Research
Brigham and Women's Hospital
2024
Harvard University
2024
Beth Israel Deaconess Medical Center
2020-2022
Cancer Research Institute
2022
University of Massachusetts Boston
2017-2020
In response to hormones and growth factors, the class I phosphoinositide-3-kinase (PI3K) signalling network functions as a major regulator of metabolism growth, governing cellular nutrient uptake, energy generation, reducing cofactor production macromolecule biosynthesis1. Many driver mutations in cancer with highest recurrence, including receptor tyrosine kinases, Ras, PTEN PI3K, pathologically activate PI3K signalling2,3. However, our understanding core metabolic program controlled by is...
The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing. In response this ongoing shortage, we began production VTM in-house in support diagnostic testing our hospital network. As laboratory was not equipped reagent production, took advantage space and personnel that became available closure the research division medical...
Reversible modification of the histone H3 N-terminal tail is critical in regulating chromatin structure, gene expression, and cell states, while its dysregulation contributes to disease pathogenesis. Understanding crosstalk between modifications nucleosomes constitutes a central challenge epigenetics. Here, we describe an engineered sortase transpeptidase, cW11, that displays highly favorable properties for introducing scarless tails onto nucleosomes. This approach significantly accelerates...
The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for SARS-CoV-2 RT-PCR testing. In response this ongoing shortage, we began production VTM in-house in support diagnostic testing our hospital network. As laboratory was not equipped reagent production, took advantage space and personnel that became available closure the research division medical center. We utilized a formulation described by CDC simple produce, did require...
Analysis of protein-protein interactions (PPIs) has become an indispensable approach to study biological processes and mechanisms, such as cell signaling, organism development, disease. It is often desirable obtain PPI information using in vivo material, gain the most natural unbiased view interaction networks. The fruit fly Drosophila melanogaster excellent platform PPIs vivo, lends itself straightforward approaches isolating material for biochemical experiments. In particular, embryos...
Abstract Reversible modification of the histone H3 N-terminal tail is critical in regulating chromatin structure, gene expression, and cell states, while its dysregulation contributes to disease pathogenesis. Understanding crosstalk between modifications nucleosomes constitutes a central challenge epigenetics. Here we describe an engineered sortase transpeptidase, cW11, that displays highly favorable properties for introducing scarless tails onto nucleosomes. This approach significantly...
Analysis of protein-protein interactions (PPIs) has become an indispensable approach to study biological processes and mechanisms, such as cell signaling, organism development, disease. It is often desirable obtain PPI information using in vivo material, gain the most natural unbiased view interaction networks. The fruit fly Drosophila melanogaster excellent platform PPIs vivo, lends itself straightforward approaches isolating material for biochemical experiments. In particular, embryos...