A5054434052- Marine Biology and Environmental Chemistry
- Echinoderm biology and ecology
- Developmental Biology and Gene Regulation
- Marine and coastal plant biology
- Marine Ecology and Invasive Species
- Marine Biology and Ecology Research
- Marine Bivalve and Aquaculture Studies
- Microplastics and Plastic Pollution
- Cephalopods and Marine Biology
- Animal Genetics and Reproduction
- Protist diversity and phylogeny
- Coral and Marine Ecosystems Studies
- Epigenetics and DNA Methylation
- Planarian Biology and Electrostimulation
- Ocean Acidification Effects and Responses
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Parasite Biology and Host Interactions
- Isotope Analysis in Ecology
- Plant Molecular Biology Research
- Spaceflight effects on biology
- Crustacean biology and ecology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Tohoku University
2008-2021
Tokyo University of Science
1998-2009
The University of Tokyo
1997-2009
California Institute of Technology
2001-2005
Tokyo Institute of Technology
1999-2001
Saint Agnes Hospital
2001
The ascidian egg is a well-known mosaic egg. In order to investigate the molecular nature of maternal genetic information stored in egg, we have prepared cDNAs from mRNAs fertilized eggs ascidian, Halocynthia roretzi. embryo were sequenced, and localization individual mRNA was examined staged embryos by whole-mount situ hybridization. data obtained database MAGEST (http://www.genome.ad.jp/magest) further analyzed. A total 4240 cDNA clones found represent 2221 gene transcripts, including at...
In the ascidian embryo, nerve cord and notochord of tail tadpole larvae originate from precursor blastomeres for both tissues in 32-cell-stage embryo. Each fate is separated into two daughter at next cleavage. We have examined mechanisms that are responsible specification through experiments involving blastomere isolation, cell dissociation, treatment with basic fibroblast growth factor (bFGF) inhibitors mitogen-activated protein kinase (MAPK) cascade. It has been shown inductive interaction...
Echinoids (sea urchins) are divided into two major groups – cidaroids (a ‘primitive’ group) and euechinoids ‘derived’ group). The a promising model species for understanding the ancestral developmental mechanisms in echinoids, but little is known about molecular of cidaroid development. In euechinoids, skeletogenic mesenchyme cell specification regulated by double-negative gate (DNG), which hesC represses transcription downstream genes (alx1, tbr ets1), thereby defining prospective region....
Concerning the evolution of deuterostomes, enteropneusts (acorn worms) occupy a pivotal role as they share some characteristics with chordates (e.g., tunicates and vertebrates) but are also closely related to echinoderms sea urchin). The nervous system in particular can be highly informative organ for evolutionary inferences, advances fluorescent microscopy have revealed overwhelming data sets on neurogenesis various clades. However, immunocytochemical descriptions juvenile particularly...
Recent progress in multiple and automated-sequencing technology allows large-scale random cDNA sequencing, the so-called EST project, various fields. In addition to collection, project requires analysis of spatiotemporal patterns gene expression a large number clones by whole-mount situ hybridization (WISH). To facilitate WISH procedures, we developed protocol for rapid uniform synthesis probes multi-well based processing. A DIG-labeled RNA probe was synthesized from PCR-amplified template...
Abstract The early stalked stages of an isocrinid sea lily, M etacrinus rotundus , were examined up to the pentacrinoid stage. Larvae induced settle on bivalve shells and cultured in laboratory developed into late cystideans. Three‐dimensional (3 D ) images reconstructed from very middle cystideans indicated that 15 radial podia composed five triplets form synchronously crescent‐shaped hydrocoel. orientation hydrocoel settled postlarvae lean posteriorly. In cystideans, orals, radials, basals...
Blastomere composition and expression profiles of wnt8 hox11/13b orthologues were examined in the primitive indirect‐developing echinoid Prionocidaris baculosa . We found that blastomere 16‐cell‐stage embryos was different from echinoids belonging to Euechinoidea, a derived group echinoids. The sizes blastomeres embryo varied, no formed “micromere quartet,” four equal‐sized micromeres. smallest usually located around vegetal pole. also significant differences early euechinoids. Unlike...
Abstract The nanos genes play important roles in the development of primordial germ cells animal species. In sea urchin, Hemicentrotus pulcherrimus , small micromere descendants specifically express HpNanos mRNA and this expression continues left coelomic pouch, which produces major component adult rudiment. study, we showed that morpholino knockdown resulted a delay primary mesenchyme cell ingression decrease number comprising pouch. Knockdown analysis chimeras whole embryos revealed...
In several model animals, the earliest phases of embryogenesis are regulated by lineage-specific genes, such as Drosophila bicoid Sea urchin (echinoid) is initiated zygotic expression pmar1, a paired-class homeobox gene that has been considered to be present only in lineage modern urchins (euechinoids). euechinoids, Pmar1 promotes endomesoderm specification repressing hairy and enhancer split C (hesC) gene. Here, we have identified basal echinoid (cidaroid) pmar1 gene, which also but not...
It has been reported that the micromeres of echinoid embryos have potential to induce an archenteron in animal cap mesomeres recombined at 16- or 32-cell stage. In present study, experiments were performed determine exact period when transmit their inductive signal respecify cell fate as endo-mesoderm. An was with a quartet micromeres, micromere-descendants cultured isolation, form recombinant embryo. The completely removed various developmental stages, resulting embryo composed only...
Echinoderms and hemichordates are sister taxa that both have larvae with tripartite coeloms. Hemichordates inherit the coelom plan ectoderm from larvae, whereas echinoderms form adult rudiment comprising rearranged coeloms a vestibule then develops into oral ectoderm. Molecular networks control patterns of central nervous system along anteroposterior (AP) axis highly conserved between chordates, respectively. In echinoderms, however, little is known about AP registry in ectoderm.We isolated...
Paramyosin is a myosin-binding protein characteristic of invertebrate animals, while troponin Ca2+-dependent regulator muscle contraction. Both proteins are widely distributed in protostomes, deuterostomes, their distribution limited; namely, presence paramyosin and absence common features echinoderm muscles, muscles chordates contain but lack paramyosin. In this study, we examined the hemichordate, acorn worm, to clarify whether animal like echinoderms or other deuterostome animals. We...