A5013225939- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Lung Cancer Treatments and Mutations
- Cancer, Hypoxia, and Metabolism
- Hepatitis B Virus Studies
- Head and Neck Cancer Studies
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- Cytokine Signaling Pathways and Interactions
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Radiopharmaceutical Chemistry and Applications
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Hepatitis C virus research
- ATP Synthase and ATPases Research
- Virus-based gene therapy research
- Computational Drug Discovery Methods
- Marine and coastal plant biology
- Pancreatic function and diabetes
- Ion Transport and Channel Regulation
- CRISPR and Genetic Engineering
National Yang Ming Chiao Tung University
2013-2024
National Tsing Hua University
2013-2024
Kaohsiung Medical University
2014-2024
National Health Research Institutes
2014-2023
Institute of Bioinformatics
2020
Iowa City Public Library
2020
California Institute of Technology
1994-2005
Stowers Institute for Medical Research
2001
University of Hertfordshire
1998
University of Southern California
1995
ABSTRACT The endo16 gene of Strongylocentrotus purpuratus encodes a secreted protein the embryonic and larval midgut. overall functional organization spatial temporal control system this are relatively well known from series earlier cis-regulatory studies. Our recent computational model for logic operations proximal region (Module A) specifies function interactions at each transcription factor target site Module A. Here, we extend sequence level analysis to adjacent region, B. is broadened...
Hepatitis B virus (HBV) infection, either acute or chronic, has been one of the leading health problems in world. To understand HBV life cycle and disease process, we set out to study regulation viral gene expression. In this paper, report characterization core promoter: two 3.5-kb transcripts, precore pregenomic, are made from it. The latter is itself a template for genome replication also encodes proteins essential both virion assembly. We identify short sequence (from nucleotides [nt]...
Food and other environmental factors affect gene expression behaviour of animals. Differences in bacterial food the longevity Caenorhabditis elegans. However, no research has been carried out to investigate whether bacteria could utilize endogenous RNAs C. elegans physiology. Here we show that two Escherichia coli noncoding RNAs, OxyS DsrA, impact on physiology downregulates che-2, leading impairment chemosensory DsrA suppresses diacylglycerol lipase F42G9.6, a decrease longevity. We also...
Significance Zebrafish Toll-like receptor (TLR) 9 (zebTLR9) and TLR21 (zebTLR21) have distinct CpG-oligodeoxynucleotide (CpG-ODN) sequence recognition profiles. The profile of zebTLR9 is more like that the TLR9s from mouse rabbit, whereas zebTLR21 similar to human TLR9 domestic animals. These two zebTLRs are requlated by UNC93B1 cooperatively mediate immunologic antimicrobial responses induced CpG-ODN in zebrafish. Our findings address molecular basis activities zebrafish provide information...
ABSTRACT The Endo16 gene of Strongylocentrotus purpuratus is expressed at the blastula stage embryogenesis throughout vegetal plate, gastrula in whole archenteron and postgastrular stages only midgut. We showed earlier that a 2300 bp upstream sequence suffices to faithfully recreate this pattern expression when fused CAT reporter gene. Here we define functional organization cis-regulatory domain, which includes over thirty high specificity binding sites, serviced by least thirteen different...
Previous studies have identified an enhancer (enhancer I) at nucleotides (nt) 1074 to 1234 in the genome of human hepatitis B virus (HBV), which locates immediately upstream from X gene. By analysis expression chloramphenicol acetyltransferase gene driven by a heterologous simian 40 early promoter, we describe identification second II) nt 1636 1741, downstream I and core With various deletions 5' end II, positive regulatory element was 1690 (the II-A element), with boundary between 1671. The...
Hepatocarcinogenesis commonly involves the gradual progression from hepatitis to fibrosis and cirrhosis, ultimately hepatocellular carcinoma (HCC). Endothelin 1 (Edn1) has been identified as a gene that is significantly up-regulated in HBx-induced HCC mice. In this study, we further investigated role of edn1 hepatocarcinogenesis using transgenic zebrafish model cell culture system. Liver-specific expression caused steatosis, fibrosis, glycogen accumulation, bile duct dilation, hyperplasia,...
Nestin is expressed in neural progenitor cells (NPC) of developing brain. Despite its wide use as an NPC marker, the function nestin embryo development unclear.As conserved zebrafish and predicted sequence clustered with mammalian orthologue, we used a model to investigate role embryogenesis. Injection morpholino (MO) into fertilized eggs induced time- dose-dependent brain eye developmental defects. morphants exhibited characteristic morphological changes including small head, eyes...
Hepatocarcinogenesis is a multistep process that starts from fatty liver and transitions to fibrosis and, finally, into cancer. Many etiological factors, including hepatitis B virus X antigen (HBx) p53 mutations, have been implicated in hepatocarcinogenesis. However, potential synergistic effects between these two factors the underlying mechanisms by which they promote hepatocarcinogenesis are still unclear. In this report, we show action of HBx mutation triggers progressive hepatocellular...
Combination of chemotherapy and radiotherapy is used to enhance local drug delivery while reducing off-target tissue effects. Anticancer doxorubicin (DOX) loaded into l-cysteine modified G4.5 dendrimer (GC/DOX) released at different pH values in the presence absence γ-radiation. Presence γ-radiation significantly improves DOX release from GC/DOX under acidic conditions, suggesting that GC a radiation-sensitive system. further evaluated by determining cytotoxicity uterine cervical carcinoma...
Summary Target of rapamycin ( TOR ) signaling is a nutrient‐sensing pathway controlling metabolism and lifespan. Although can be activated by metabolite diacylglycerol DAG ), phosphatidic acid PA the precise genetic mechanism through which influences lifespan remains unknown. metabolized to either via action kinase or 2‐arachidonoyl‐ sn ‐glycerol lipase DAGL ). Here, we report that in D rosophila C aenorhabditis elegans, overexpression / ina E dagl‐1 knockdown DGK rdgA dgk‐5 extends enhances...
Hepatocellular carcinoma (HCC) ranks as the fourth leading cause of cancer-related deaths worldwide. Sorafenib was only U.S. Food and Drug Administration (FDA) approved drug for treating advanced HCC until recently, so development new target therapy is urgently needed. In this study, we established a zebrafish screening platform compared therapeutic effects two multiple tyrosine kinase inhibitors, 419S1 420S1, with Sorafenib. All three compounds exhibited anti-angiogenesis abilities in...
With-no-lysine (K)-1 (WNK1) is the founding member of family four protein kinases with atypical placement catalytic lysine that play important roles in regulating epithelial ion transport. Gain-of-function mutations WNK1 and WNK4 cause a mendelian hypertension hyperkalemic disease. ubiquitously expressed essential for embryonic angiogenesis mice. Increasing evidence indicates role WNK tumorigenesis at least partly by stimulating tumor cell proliferation. Here, we show human hepatoma cells...
Hepatitis B virus X antigen plays an important role in the development of human hepatocellular carcinoma (HCC). The key regulators controlling temporal downstream gene expression for HCC progression remains unknown. In this study, we took advantage systems biology approach and analyzed microarray data HBx transgenic mouse as a screening process to identify differentially expressed genes applied software Pathway Studio potential pathways involved HCC. Using subnetwork enrichment analysis,...
The deregulated nonoxidative pentose phosphate pathway (PPP) is known to promote oncogenesis, but the molecular mechanism remains unknown. Here, we report that human ribose-5-phosphate isomerase A (RPIA) plays a role in hepatocellular carcinoma (HCC). significant increase RPIA expression was detected both tumor biopsies of HCC patients and liver cancer tissue array. Importantly, clinicopathological analysis indicated mRNA levels were highly correlated with clinical stage, grade, size, types,...
The WNK1 (WNK lysine deficient protein kinase 1) is a serine/threonine with emerging roles in cancer. causes hypertension and hyperkalemia when overexpressed cardiovascular defects ablated mice. In this study, the role of Wnk1 angiogenesis was explored using zebrafish model. There are two wnk1 isoforms, wnk1a wnk1b, both contain all functional domains found human protein. Both isoforms expressed embryo at initiation posterior cardinal vein (PCV), similar to fms-related tyrosine 4 (flt4)....