A5026837546- Nerve injury and regeneration
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Connexins and lens biology
- Nicotinic Acetylcholine Receptors Study
- Retinal and Macular Surgery
- Corneal surgery and disorders
- RNA regulation and disease
- Photoreceptor and optogenetics research
- Pain Mechanisms and Treatments
- Axon Guidance and Neuronal Signaling
- S100 Proteins and Annexins
- Retinal Diseases and Treatments
- Mitochondrial Function and Pathology
- Corneal Surgery and Treatments
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Drug-Induced Ocular Toxicity
- Spaceflight effects on biology
- Inflammatory mediators and NSAID effects
- Cell death mechanisms and regulation
- Alkaline Phosphatase Research Studies
- Retinopathy of Prematurity Studies
Medical University of Silesia
2013-2024
Glaucoma is a chronic disease that causes structural and functional damage to retinal ganglion cells (RGC). The currently employed therapeutic options are not sufficient prevent vision loss in patients with glaucoma; therefore, there need develop novel therapies, which requires the creation of functional, repeatable easy-to-utilize animal models for use pre-clinical studies. available ensure only low moderate optic nerves, high variation outcomes poor repeatability. We have developed an...
Generator of spatial magnetic field is one most recent achievements among the magnetostimulators. This apparatus allows to obtain rotating field. new method may be more effective than other widely used techniques magnetostimulation and magnetotherapy. We investigated influence alternating, on regeneration crushed rat sciatic nerves. Functional morphological evaluations were used. After crush injury right nerve, Wistar C rats (n = 80) randomly divided into four groups (control three...
Introduction ELAVL1/HuR is a keystone regulator of gene expression at the posttranscriptional level, including stress response and homeostasis maintenance. The aim this study was to evaluate impact hur silencing on age-related degeneration retinal ganglion cells (RGC), which potentially describes efficiency endogenous neuroprotection mechanisms, as well assess exogenous capacity -silenced RGC in rat glaucoma model. Methods consisted vitro vivo approaches. In , we used B-35 investigate,...
To characterize newly discovered electrical synapses, formed by connexin (Cx) 36 and 45, between neighbouring axons within the optic nerve head.Twenty-five Wistar rats were killed CO2 inhalation. Proximal distal (ON) stumps collected processed for immunostainings, electron microscopy (EM) with immunogold labelling, PCR Western blots (WB). Additional 15 animals deeply anaesthetized, flash visual evoked potentials (fVEP) after retrobulbar injection of saline (negative control) or 100 μm...
Abstract Glaucoma is an optic neuropathy that leads to irreversible blindness. Because the current therapies are not sufficient protect against glaucoma-induced visual impairment, new treatment approaches necessary prevent disease progression. Cell transplantation techniques currently considered be among most promising opportunities for nervous system damage treatment. The beneficial effects of undifferentiated cells have been investigated in experimental models glaucoma, however experiments...
Preclinical toxicity screening of the new retinal compounds is an absolute requirement in pathway further drug development. Since neuron cultivation and vivo studies are relatively expensive time consuming, we aimed to create a fast reproducible ex system for screening. For this purpose, used rat explant culture that was retrogradely labeled with FluoroGold before isolation. Explants were exposed toxic concentration gentamicin ciliary neurotrophic factor (CNTF), known neuroprotective agent....
Glaucoma is thought to be the main cause of severe visual impairment or permanent loss vision. Current therapeutic strategies are not sufficient protect against glaucoma. Thus, new therapies and potential novel targets must developed achieve progress in treatment this insidious disease. This study was undertaken verify whether time administration an extract from predegenerated rat sciatic nerves as well exposure onto retinal ganglion cells (RGCs) influences survival RGCs a glaucoma model. We...
Prostaglandin analogs are the first line of treatment in patients with glaucoma. Recently, many preservative-free prostaglandin have been marketed to increase their tolerance chronic use. However, potentially safer formulations reported induce inflammation within ocular surface and adnexa, associated pronounced activation tissue macrophages.
We investigated neurotrophic activity of extracts from pre-degenerated and non-pre-degenerated peripheral nerves (complete with fractions narrower range molecular weight) on the injured hippocampus.The experiment was carried out male Wistar C rats. The complete or different ranges weight were introduced to site injury autologous connective tissue chambers. examined DiI-labeled hippocampal cell AChE-positive nerve endings assess regeneration intensity.The highest number labeled cells observed...
AbstractAbstract Objectives: Retinal ganglion cells (RGCs) of adult rats are unable to regenerate their axons after optic nerve injury and soon they enter the pathway apoptosis. They may, however, survive new in response application specific peripheral extracts that presumably contain a range neurotrophic substances. One recognized substances proven activity is brain-derived factor (BDNF).We have investigated whether blocking BDNF post-microsomal fractions obtained from 7 day pre-degenerated...
In contrast to peripheral nerves, central neurons do not regrow spontaneously after injury. Our previous studies showed that transplantation of degenerating nerves or their extracts can induce regeneration in the injured nervous system. Non-predegenerated show much weaker neurotrophic activity. The aim present work was examine quantitatively and qualitatively protein composition rat sciatic nerve extracts.The experiments were carried out on male Wistar C rats. Distal fragments collected...
The purpose of the study was to examine influence spatial variable magnetic field (induction: 150–300 µT, 80–150 20–80 µT; frequency 40 Hz) on neuropathic pain after tibial nerve transection. experiments were carried out 64 male Wistar C rats. exposure animals performed 1 d/20 min., 5 d/week, for 28 d. Behavioural tests assessing intensity allodynia and sensitivity mechanical thermal stimuli conducted d prior surgery 3, 7, 14, 21 surgery. extent autotomy examined. Histological...
Purpose To investigate neuroprotective and pro-regenerative potential of predegenerated Schwann cells towards RGCs in ex vivo retinal explants. Methods In this study we used two models rat explants culture - reversed from P2 Wistar rats pups insert 4 weeks old rats. Both types were cultured Neurobasal A medium with supplements (B-27, N2 GlutaMax). way prepared 32 (16 2 µl suspension (104 cells) dropped on the each surface, another 16 served as control treated DPBS) 30 (10 them standard...
Purpose To investigate impact of transplanted peripheral glia on neurites outgrowth and synaptogenesis in adult rat retina associated with ongoing optic neuropathy. Methods In this study we used two models retinal neurodegeneration – degeneration denervation represented by explants culture glaucomatous model ocular hypertension. Retinal were cultured standard supplemented Neurobasal A medium (n = 48). Ocular hypertension has been induced using beads occlusive 10). both ex vivo approaches...