To assess the feasibility and reliability of Laser Speckle Flowgraphy (LSFG) to measure ocular perfusion in a sample healthy white subjects elucidate age-dependence parameters obtained.This cross-sectional study included 80 eyes healthy, non-smoking Western European descent between 19 79 years age. A commercial LSFG instrument was applied blood flow at optic nerve head (ONH) three successive times before after pharmacological pupil dilation. The mean blur rate (MBR), relative velocity,...

The aim of this study was to compare retinal oxygen extraction in individuals with diabetes no or mild non-proliferative diabetic retinopathy and healthy age- sex-matched volunteers. A total 24 participants type 1 volunteers were included cross-sectional study. Retinal measured by combining blood flow measurements using a custom-built bi-directional Doppler optical coherence tomography system saturation spectroscopic reflectometry. Based on previously published mathematical modelling, the...

The aim of the present study was to investigate regulation blood flow (BF) in optic nerve head (ONH) and a peripapillary region (PPR) during an isometric exercise-induced increase ocular perfusion pressure (OPP) using laser speckle flowgraphy (LSFG) healthy subjects. For this purpose, total 27 subjects included study. Mean blur rate tissue (MT) measured ONH PPR as well relative volume (RFV) retinal arteries (ART) veins (VEIN) LSFG. All participants performed exercise for 6 minutes which MT...

Purpose: This study evaluated the validity of retinal perfusion measurements using laser speckle flowgraphy (LSFG) by means in vitro experiments and direct comparison with dual-beam Doppler optical coherence tomography (D-OCT) a healthy Caucasian population. Methods: The flow velocity scattering solution pumped through glass capillary was measured at 17 different velocities (range, 0.5–47 mm/s) LSFG. within produced computer-controlled infusion pump. In vivo, three consecutive LSFG scans...

Purpose: The aim of this mechanistic clinical study was to explore the effect water-free perfluorohexyloctane eye drops on tear film thickness (TFT) in patients with dry disease (DED). Methods: Forty-eight mild moderate DED participated randomized, single-masked, observer-blinded parallel group a 1:1 ratio receive either or unpreserved 0.9% saline solution (Hydrabak®, Thea, France) 4 times daily both eyes for weeks. A custom-built ultrahigh-resolution optical coherence tomography system used...

Background/aims There is considerable interest in novel techniques to quantify choroidal blood flow (CBF) humans. In the present study, we investigated a technique measure CBF based on laser speckle flowgraphy (LSFG) healthy subjects. Methods This study included 31 eyes of healthy, non-smoking subjects aged between 19 and 74 years. A commercial LSFG instrument was used vessel diameter (CVD) relative volume (RFV) vessels that were identified fundus photos, an approach previously only for...

Abstract Purpose To compare the effect of a single drop different lubricant eye gels on tear film thickness ( TFT ) as measured with ultrahigh‐resolution optical coherence tomography UHR ‐ OCT in patients mild‐to‐moderate dry disease DED ). Methods The study followed randomized, single‐masked, observer‐blinded parallel group design. Patients received dose either unpreserved trehalose 3% + hyaluronic acid 0.15% TH ), 0.2% HA or polyethylene glycol 0.4% propylene 0.3% PP drops. In total, 60...

Corneal abrasions are frequent after standard (epithelium-off [epi-off]) corneal collagen cross-linking (CXL) in patients with progressive keratoconus. A new matrix therapy agent (ReGeneraTing Agent [RGTA]) has been developed to promote wound healing.To assess the effect of type on healing epi-off CXL keratoconus.This double-masked randomized clinical trial enrolled 40 keratoconus undergoing from July 18, 2014, October 21, 2015, when last follow-up was completed. The analysis...

Purpose: To investigate the age-dependence of total retinal blood flow and oxygen extraction in healthy subjects determine their possible correlations with structural optical coherence tomography (OCT) parameters. Methods: This observational cross-sectional study consisted 68 (mean ± SD age, 45.6 16.3 years; 47% female). Total was calculated based on measurement using bi-directional Doppler OCT saturation spectroscopic reflectometry. Retinal nerve fiber layer thickness measured OCT, number...

Abnormal autoregulation of optic nerve head blood flow (ONHBF) has been postulated to play an important role in primary open-angle glaucoma (POAG). We used laser Doppler flowmetry (LDF) estimate quantitatively the ONHBF and compared between patients healthy controls during isometric exercise.Forty with POAG 40 age- sex-matched subjects underwent three periods exercise, each consisting 2 min handgripping. Optic was measured continuously using LDF. Systemic pressure, intraocular pressure...

The coupling between neural activity and blood flow is a physiological key principle of ocular regulation. current study was performed to investigate whether Laser speckle flowgraphy (LSFG), commercially available technique for measuring flow, capable assess flicker-induced haemodynamic changes in the retinal optic nerve head (ONH) circulation.Twenty healthy subjects were included this cross sectional study. A commercial LSFG instrument used measure at ONH as well vessels before during...

Purpose: This study investigated the effect of single administration 2 different hyaluronic acid-based topical lubricants on tear film thickness (TFT) in patients with moderate-to-severe dry eye disease (DED). Methods: Sixty were randomized to receive drops containing unpreserved sodium hyaluronate, triglycerides, and phospholipids (HTP), hyaluronate (HA), or chloride (NaCl). TFT lipid layer (LLT) assessed before at defined time points after instillation a custom-built ultrahigh-resolution...

Purpose . This randomized, observer-masked, crossover study investigated the effect of two hyaluronic acid/trehalose-based containing formulations, with different physical properties, on signs and symptoms in patients moderate to severe dry eye disease (DED). Methods In one group, received a mixture sodium hyaluronate trehalose (HT, Thealoz Duo®) for use during day. other more viscous formulation consisting acid, trehalose, carbomer (HTC-gel, Duo Gel) pro re nata. Both groups used HTC-gel...

To compare retinal vessel calibers extracted from phase-sensitive optical coherence tomography (OCT) images with as obtained the Retinal Vessel Analyzer (RVA).Data previously published studies in 13 healthy subjects breathing room air (n = 214 vessels) and 7 100% oxygen 101 were used. OCT phase measured vertically along axis by three independent graders. The data RVA fundus corrected for magnification to obtain absolute values.The average diameter during normoxia was lower than (83.8 ± 28.2...

Abstract Purpose We aimed to describe the global and localized correlations among visual field (VF) sensitivity, optic nerve head (ONH) perfusion measured by laser speckle flowgraphy (LSFG) neural structure optical coherence tomography (OCT) in open‐angle glaucoma (OAG) compare floor effect for LSFG OCT. Methods Cross‐sectional, multicenter study including one eye each from fifty OAG patients (mean age 69.3 years; average VF mean deviation, MD, −8.5 dB, range −25.17 0.85 dB) fifty‐one...

Purpose: Long-term glaucoma therapy with preservative-containing eye drops may impact ocular surface health. This study was performed to investigate whether a switch from preserved topical prostaglandin preservative-free tafluprost improves precorneal tear film thickness in patients or hypertension. Methods: A total of 30 who were under monotherapy for at least 6 months included. Patients then switched unpreserved once daily. Tear measured baseline and 4 12 weeks after change an...

Objective: To compare total retinal oxygen extraction between patients with primary open-angle glaucoma (POAG) and healthy control subjects. Design: A prospective, single-center, cross-sectional, case–control study performed at the Medical University of Vienna. Subjects: Forty POAG 40 age- sex-matched Methods: Total blood flow was measured using Doppler optical coherence tomography (OCT). Retinal arterial venous saturation reflectance spectroscopy. From these parameters, content in...

Purpose To evaluate the reliability and feasibility of Laser Speckle Flowgraphy (LSFG) measurement ocular blood perfusion in a group healthy Caucasian descents to explicate age‐dependence obtained parameters. Methods A population 80 eyes healthy, non‐smoking subjects descent aged between 19 79 years was included this cross‐sectional study. commercially available LSFG system used measure optic nerve head (ONH) flow three successive times both miosis mydriasis. The mean blur rate (MBR),...

Laser speckle flowgraphy (LSFG) is a promising technique for the two-dimensional, non-invasive assessment of ocular blood flow. We set out to investigate use this in healthy Caucasians. First, we evaluated validity retinal perfusion measurements by direct comparison with dual-beam Doppler optical coherence tomography (D-OCT). Relative flow volume (RFV), main output parameter LSFG, was significant agreement absolute as obtained from D-OCT arteries (r = 0.69, P 0.001) and veins 0.74, < 0.001)....