A5102951207- Diabetes and associated disorders
- Diabetes Management and Research
- Congenital heart defects research
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Pluripotent Stem Cells Research
- Innovations in Medical Education
- T-cell and B-cell Immunology
- Developmental Biology and Gene Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Congenital gastrointestinal and neural anomalies
- Ion channel regulation and function
- Medical Education and Admissions
- COVID-19 Clinical Research Studies
- Diversity and Career in Medicine
- Wnt/β-catenin signaling in development and cancer
- Immune cells in cancer
Penn State Milton S. Hershey Medical Center
2024
University of Florida
2015-2021
Washington University in St. Louis
2019
Indiana University School of Medicine
2013
Indiana University Bloomington
2013
Indiana University
2013
Neural crest cells are multipotent progenitor that can generate both ectodermal cell types, such as neurons, and mesodermal smooth muscle. The mechanisms controlling this fate choice not known. basic Helix-loop-Helix (bHLH) transcription factor Twist1 is expressed throughout the migratory post-migratory cardiac neural crest. ablation or mutation of Twist-box causes differentiation ectopic neuronal cells, which molecularly resemble sympathetic ganglia, in outflow tract. interacts with...
Most natural history models for type 1 diabetes (T1D) propose that overt hyperglycemia results after a progressive loss of insulin-secreting β-cell mass and/or function. To experimentally address this concept, we prospectively determined morning blood glucose measurements every other day in multiple cohorts (total n = 660) female NOD/ShiLtJ mice starting at 8 weeks age until onset or 26 age. Consistent with notion, majority developed (354 489 [72%]) displayed increase transient excursions...
NK cells can recognize target such as virus-infected and tumor through integration of activation inhibitory receptors. Recognition by lead to direct lysis the cell production signature cytokine IFN-γ. However, it is unclear whether stimulation receptors alone sufficient for IFN-γ production. In this study, we show that receptor engagement requires additional signals optimal production, which could be provided IFN-β or IL-12. Stimulation murine with soluble Abs directed against NK1.1, Ly49H,...
Previously, we demonstrated low-dose antithymocyte globulin (ATG) and granulocyte colony-stimulating factor (GCSF) immunotherapy preserved C-peptide for 2 years in a pilot study of patients with established type 1 diabetes (n = 25). Here, evaluated the long-term outcomes ATG/GCSF participants 5 available follow-up data 15). The primary end point was area under curve (AUC) during 2-h mixed-meal tolerance test. After years, there were no statistically significant differences AUC when comparing...
Aims: Reactive oxygen species (ROS) are critical in driving the onset of type 1 diabetes (T1D). Ablation ROS derived from phagocytic NADPH oxidase 2 is protective against autoimmune non-obese diabetic (NOD) mice. However, mechanisms 2-derived T1D pathogenesis need to be elucidated. Here, we have examined role Ncf1 (the regulatory subunit 2) dendritic cells (DC). Results: Ncf1-mutant DCs exhibit reduced ability activate autoreactive CD8+ T despite no difference co-stimulatory molecule...
The chronic failure to significantly increase the number of underrepresented minorities (URM) in medicine requires that we look for new mechanisms channelling URM students through pre-medical education and into medical school. One potential mechanism is scribing, which involves a person helping physician engage real-time documentation electronic record.
OBJECTIVES/SPECIFIC AIMS: Understand the immunomodulatory effects of anti-thymocyte globulin (ATG) and granulocyte colony stimulating factor (G-CSF) on type 1 diabetes patients using samples in preclinical model, nonobese diabetic mouse. METHODS/STUDY POPULATION: Flow cytometry analysis phase peripheral blood treatment mouse with ATG GCSF flow immune organs (spleen, lymph nodes, blood, bone marrow). RESULTS/ANTICIPATED RESULTS: Changes both innate adaptive cell subsets including plasmacytoid...
Investigations into the role of neutrophil in type 1 diabetes (T1D) have provided controversial results. It is thus essential to understand how neutrophils change with age and if these numbers are linked by T1D. To do this, we utilized whole blood count data from our database, which includes 2619 unique individuals, pancreata slices nPOD donors, points Neulasta T1D clinical trial, NCT00662519. We first examined changes neutrophils, total white cells (WBC), lymphocytes individuals T1D, T2D,...