A5070204465- Genetic diversity and population structure
- Wildlife Ecology and Conservation
- Genetic and phenotypic traits in livestock
- Identification and Quantification in Food
- Ruminant Nutrition and Digestive Physiology
- Biochemical Analysis and Sensing Techniques
- Genomics and Phylogenetic Studies
- melanin and skin pigmentation
- Reproductive Physiology in Livestock
- Animal Ecology and Behavior Studies
- Meat and Animal Product Quality
- Skin Protection and Aging
- Fish Biology and Ecology Studies
- Isotope Analysis in Ecology
- Genetic Mapping and Diversity in Plants and Animals
- Bone and Dental Protein Studies
- Nuclear Physics and Applications
- Animal Genetics and Reproduction
- Forensic and Genetic Research
- Radioactive contamination and transfer
- RNA and protein synthesis mechanisms
- Ecology and Vegetation Dynamics Studies
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Bat Biology and Ecology Studies
Yamagata University
2014-2025
Yamagata University Hospital
2010-2020
Ishinomaki Senshu University
1995-2003
University of the Ryukyus
2000
Kyushu University
2000
Senshu University
1999
Case Western Reserve University
1990
Tohoku University
1962-1986
Okayama University
1984
National Institute of Animal Health
1981
Abstract We assessed genetic differentiation and diversity in 14 populations of sika deer ( Cervus nippon ) from Japan four introduced to the UK, using nine microsatellite loci. observed extreme levels significant differences between populations. Our results do not support morphological subspecies designations, but are consistent with previous mitochondrial DNA analyses which suggest existence two genetically distinct lineages Japan. The source UK was identified as Kyushu. underlying...
Dibutyryl adenosine 3',5'-monophosphate (dibutyryl cyclic AMP) induced eumelanin synthesis in hair bulb melanocytes of recessive yellow (e/e) mice vitro, whereas alpha-melanocyte-stimulating hormone (alpha-MSH) did not. In contrast, the lethal (Ay/a) produced response to both dibutyryl AMP and alpha-MSH. These results suggest that e locus controls a mechanism determines function an alpha-MSH receptor.
1. The rumen adapts to increased food intake by the hyperplasia of epithelial cells. 2. Volatile fatty acids (VFA) stimulate cell mitosis sheep ruminal epithelium in vivo. 3. Since VFA generally inhibit proliferation vitro insulin was proposed this study as possible mediator mitotic stimulation 4. Infusions (6 h) (0.125 U/kg per plus glucose (300 mg/kg ( n 5), and alone 2) resulted higher index biopsied (MI) during 3 or 6 24 h after start infusion, plasma immunoreactive (IRI) (PG) infusion....
Abstract The composition of myofiber types varies within thigh muscles chickens. present study was designed to determine whether or not were distributed uniformly across the diameter Cross sections from middle portions used histochemically examine differences in distribution and muscles. Myofibers that reacted moderately (M) strongly (S) for myosin adenosine triphosphatase (ATPase) after preincubation at pH 4.3 classified as type I. Type I myofibers weakly (W), (M), ATPase 10.6; these...
A partial sequence of the mitochondrial cytochrome-b gene was determined for seven subspecies sika deer (Cervus nippon) in Japanese Islands. Nine DNA genotypes were distinguishable among sampled. Sequence analysis revealed two major phylogenetic groups comprised northern (Hokkaido-Honshu) and southern (Kyushu) local populations. Estimated nucleotide divergence between found populations ≤1.1%, which indicated that genetic differentiation within this group occurred recently. Phylogenetic data...
Abstract Non‐invasive DNA genotyping using hair samples has become a common method in population surveys of Asiatic black bears ( Ursus thibetanus ) Japan; however, the accuracy data rarely been discussed empirical studies. Therefore, we conducted large‐scale pilot study to examine and sought an efficient way error‐checking hair‐trapping data. We collected 2,067 samples, successfully determined genotypes 1,245 identified 295 individuals. The were further divided into 3 subsets according...
To investigate the factors that influence resting site preference of Japanese dormouse Glirulus japonicus, we determined effect tree resources in a microhabitat on use nest boxes as daytime sites. The study area, which was Mt. Nagakura National Forest central Japan, divided into 200 plots. In each plot, box specially designed to harbor dormice attached trunk or branch tree. All living trees around were recorded and categorized 3 resource types: food, nesting materials, other resources. We...
We examined the population genetic structure of Asian black bear (Ursus thibetanus) in a large continuous habitat northern Japan. To determine how subdivision relates to management units (MUs) proposed by Ministry Environment, variation mitochondrial DNA control region (mtDNA-CR) and seven autosomal microsatellite loci was assessed bears captured southern Tohoku. Geographical distribution subpopulations using landscape analyses find best-fit model based on maximum entropy (MaxEnt) prediction...
Abstract The levels of tyrosinase activities, cross‐reacting material (TY‐CRM), and mRNA were determined for skins from five congenic strains mice ( Mus musculus ) with different coat colors. dopa oxidase activity melanin formation directly proportional to the abundance TY‐CRM in all mutants. enzyme activities increased brown b / dilute d mice, while they reduced agouti A pink‐eyed dilution p animals. In albino c scarcely detectable, no was observed. Contrary these, mutants almost same as...
Abstract Alleles at the agouti locus determines type of pigment produced in hair‐bulb melanocytes. In order to analyze mechanism gene function, an attempt was made induce shift melanin synthesis vitro. Skin explants from newborn yellow mice with genotype A y / a were cultured method using membrane‐filter and roller tube. Production black hair bulbs observed when presence α‐melanocyte stimulating hormone (α‐MSH). Electron‐microscopic observation indicates that induced pigments are eumelanin...
Previous investigations of phylogeny in Cervus recovered many clades without whole genomic support. In this study, the genetic diversity and 5 species (21 subspecies/populations from C. unicolor, albirostris, nippon, elaphus eldii) genus were analyzed using reduced-representation genome sequencing. A total 197,543 SNPs identified with an average sequencing depth 16 x. 21 SNP matrices for each subspecies/population 1 matrix individual analysis constructed, respectively. Nucleotide...
Variations in the mitochondrial DNA (mtDNA) of Sika deer (Cervus nippon) from seven localities Japan were examined to assess degree genetic differentiation among subspecies. The entire mtDNA C. n. centralis, which is about 16,600 base pairs length, was cloned into plasmids. Using various clones as hybridization probes, we constructed restriction maps for 59 and identified six different genotypes. genotypes observed subspecies centralis Honshu Island (the mainland) distinct that same Tsushima...
Marine lakes in the Palau Islands are known to harbor unique marine fauna that have remained isolated since formation of after Last Glacial Maximum. We analyzed mussels from located on different islands and conducted morphological, phylogenetic population genetic characterization clarify their evolutionary history. The were morphologically classified into three differentiated morphs: NS, ON, MC. Their common characteristics consistent with Brachidontes-Hormomya complex Mytilidae family....
Nucleotide sequences of sika deer (Cervus nippon) collected from the eastern part Shikoku Island were investigated & compared with those other areas. sequence whole D-loop region mitochondrial DNA was determined by direct sequencing technique for each sample. The phylogenetic tree constructed indicates that are divided into two distinct lineages: northern Japan group and southern group. Proportion lineage higher in northeastern sampling area. There no border between distribution lineages,...
Deer have been a major resource for human populations thousands of years. Anthropogenic activities, such as hunting, influenced the genetic structure and distribution deer populations. In Japan, wild Japanese sika (Cervus nippon) hunted since ancient times but also historically protected sacred animals in several sanctuaries. Sika over thousand years religious sanctuary around Kasuga Taisha Shrine on Kii Peninsula, located center Japan. Here, we used short sequence repeats (SSR)...
Genetic variations within a population of the Japanese sika deer, Cervus nippon, on Kinkazan Island were studied by microsatellite analysis. Seventeen pairs polymerase chain reaction primers designed for several species ungulates successfully amplified polymorphic DNA in deer. About 20% was sampled and genotyped nine diagnostic loci. Alleles at those loci found to be under Hardy-Weinberg equilibrium. To determine whether deer have reduced level genetic variability, an average heterozygosity...
Abstract Bartonella quintana bacteremia was detected in 6 (13.3%) of 45 wild-caught Japanese macaques (Macaca fuscata). Multilocus sequence typing the isolates revealed that were infected with a new and specific B. type. Free-ranging thus represent another natural reservoir quintana.
Simple sequence repeats (SSRs) have become one of the most popular molecular markers for population genetic studies. The application SSR has often been limited to source species because loci are too labile be maintained in even closely related species. However, a few extremely conserved reported. Here, we tested presence acanthopterygian fishes, which include over 14 000 species, by comparing genome sequences four fishes. We also examined comparative genome-derived SSRs (CG-SSRs) their...