A5082918718- RNA Research and Splicing
- Amyotrophic Lateral Sclerosis Research
- RNA and protein synthesis mechanisms
- Neurogenetic and Muscular Disorders Research
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Neurological and metabolic disorders
- Microbial metabolism and enzyme function
- Cell Image Analysis Techniques
- Advanced MRI Techniques and Applications
- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- RNA regulation and disease
- biodegradable polymer synthesis and properties
- Hereditary Neurological Disorders
- Advanced Fluorescence Microscopy Techniques
- Plant Virus Research Studies
- Autophagy in Disease and Therapy
- Diabetic Foot Ulcer Assessment and Management
Weizmann Institute of Science
2020-2024
Haifa Medical Center
2024
Bar-Ilan University
2014-2015
Goodman (Japan)
2015
Core promoter elements play a pivotal role in the transcriptional output, yet they are often detected manually within sequences of interest. Here, we present 2 contributions to detection and curation core given sequences. First, Elements Navigation Tool (ElemeNT) is user-friendly web-based, interactive tool for prediction display putative their biologically-relevant combinations. Second, CORE database summarizes ElemeNT-predicted near CAGE RNA-seq-defined Drosophila melanogaster...
Abstract Bloom's syndrome (BLM) protein is a known nuclear helicase that able to unwind DNA secondary structures such as G-quadruplexes (G4s). However, its role in the regulation of cytoplasmic processes involve RNA (rG4s) has not been previously studied. Here, we demonstrate BLM recruited stress granules (SGs), which are biomolecular condensates composed RNAs and RNA-binding proteins. enriched SGs upon different conditions an rG4-dependent manner. Also, show unwinds rG4s acts negative...
RNA G-quadruplexes (rG4s) are secondary structures, which formed by guanine-rich sequences and have important cellular functions. Existing computational tools for rG4 prediction rely on specific sequence features and/or were trained small datasets, without considering stability information, therefore sub-optimal. Here, we developed rG4detector, a convolutional neural network to identify potential rG4s in transcriptomics data. rG4detector outperforms existing methods both predicting detecting...
Abstract Systematic assessment of organelle architectures in cells, known as the organellome, could provide valuable insights into cellular states and disease pathologies but remains largely uncharted. Here, we devised a novel pipeline combining self-supervised deep learning transfer to generate Neuronal Organellomics Vision Atlas (NOVA). Analyzing over 1.5 million confocal images 24 distinct membrane-bound membrane-less organelles human neurons, enable simultaneous evaluation all...
Abstract Introduction Hyperbaric oxygen has been used as a medical treatment tool in hyperbaric chambers and is an integral part of professional combat divers’ activity. In extreme cases, exposure to can develop central nervous system toxicity (CNS-OT), which leads seizures eventually death. CNS-OT caused by neuronal hyperactivity due high levels, potentially damaging brain cells including the blood-brain barrier (BBB). However, effect levels on healthy BBB not characterized directly yet....
Summary Cytoplasmic stress granules (SG) form in response to a variety of cellular stresses by phase-separation proteins associated with non-translating mRNAs. SG provide insight into the biology neurodegeneration, including amyotrophic lateral sclerosis (ALS) because they approximate some molecular conditions for nucleation insoluble aggregates neuropathological inclusions. Whereas much has been learned about formation, major gap remains understanding compositional changes undergo during...
Cytoplasmic stress granules (SG) form in response to a variety of cellular stresses by phase-separation proteins associated with non-translating mRNAs. SG provide insight into the biology neurodegeneration, including amyotrophic lateral sclerosis (ALS) because they approximate some molecular conditions for nucleation insoluble aggregates neuropathological inclusions. Whereas much has been learned about formation, major gap remains understanding compositional changes undergo during normal...
Abstract The non-coding genome is substantially larger than the protein-coding but largely unexplored by genetic association studies. Here, we performed region-based burden analysis of >25,000 variants in untranslated regions 6,139 amyotrophic lateral sclerosis (ALS) whole-genomes and 70,403 non-ALS controls. We identified Interleukin-18 Receptor Accessory Protein (IL18RAP) 3′UTR significantly enriched genomes, replicated an independent cohort, associated with a five-fold reduced risk...