MicroRNAs (miRNAs) play important roles in many biological processes and are regarded as promising cancer biomarkers. Herein, a highly specific, one-step, rapid miRNAs detection strategy with attomolar sensitivity has been developed on the basis of target-triggered three-way junction (3-WJ) structure polymerase/nicking enzyme synergetic isothermal quadratic DNA machine (ESQM). To this end, 3-WJ probes (primer template) designed to selectively recognize target miRNA form stable trigger ESQM,...

The perforated caisson structure is used in ports and waterways for its wave dissipation performance. This study modifies the traditional SPH method using Riemann solution CSPH to examine interaction between waves caissons. A comparative analysis of single- double-chambered caissons was conducted, considering various conditions. Results show that best occurs when perforation ratio front plate higher than back plate, chamber narrower chamber. Equal widths are beneficial adapting diverse...

Herein, we propose a novel and universal biosensing platform based on polymerase-nicking enzyme synergetic isothermal quadratic DNA machine (ESQM). This tactfully integrates two signal amplification modules, strand displacement (SDA) nicking (NESA), into one-step system. A bifunctional probe with stem-loop structure was designed to be partly complementary the SDA product digested substrate of NESA for bridging NESA. ESQM can performed by using only enzymes buffer involved in module. In...

Abstract Background The purpose of this study was to compare the outcome, complications and survival commonly used surgical reconstructions proximal humerus after intrarticular tumour resection in our hospital. Methods Between 1998 2010, 41 consecutive humeral using prosthesis (group P, n = 25) or recycled pasteurized autograft combined with non-vascularised fibula B, 16) were performed. Results mean follow-up 57.7 months. Fourteen patients (8 group P 6 B) died during period,...

From a mesoscopic view, recycled concrete (RC) can be taken as multi-phase composition which is consisted of aggregate, mortar and the interface between them. The three-dimensional grading curve aggregate determined by referring to Fuller curve. And with Monte Carlo method, meso-sample RC developed randomly. By gradually mesh generation randomly distributed aggregates, interfaces are meshed in turn. Finally, meso finite element model just generated, when (RA) mixing proportion 0%,40%,70%...

Skin corrugation and tandem configuration are two distinct features that characterize the flow around dragonfly wings. In contrast to smooth airfoil single pair of wings conventional airplanes, corrugated surfaces influence aerodynamics both locally globally. this article, several kinds doubly- wing configurations were designed, then computational investigations based on wind tunnel experiments conducted investigate aerodynamic characteristics these models. Computational simulations using...

Effects of the aeroelasticity on performance hypersonic inlet have been investigated numerically in this study. The has simulated using coupled computational fluid dynamics/computational structural dynamics method, which is solved by in-house code. unsteady Reynolds-averaged Navier–Stokes equations simulation, and modal method adopted simulation. Two cases utilized to validate numerical method. Finally, for plate with different thicknesses. effects parameters flow structure discussed detail....

Optimization of aerodynamic shapes using numerical methods has always been engineering interest. This paper presents a highly efficient optimization method for hypersonic wings based on local piston theory. The objective the is to improve characteristics original airfoils or generated from NACA0012 while satisfying constraints structure requirements. In procedure, genetic algorithm employed optimum search, Hicks–Henne bump function used airfoil geometrical modification, and theory unsteady...

As a typical brittle material, glass is widely used in construction, transportation, shipbuilding, aviation, aerospace and other industries. The unsafe factors of mainly come from its rupture. Thus, establishing set o... | Find, read cite all the research you need on Tech Science Press

The flow structures and surface pressure distributions on corrugated airfoils significantly differed from those a conventional, smooth airfoil. An unsteady, two-dimensional computational simulation was carried out to investigate the behavior associated aerodynamic performance of group with different levels waviness at angles attack 0° 20° an interval 2° low Reynolds number regime (Re = 1.2 × 105) were quantitatively compared its counterpart. Time-averaged coefficients demonstrated that have...

Activated cement with the characters of low water demand and high activity was made Portland clinker dehydrate-gypsum liquid superplasticizer which were used as grinding additive in process. Three states effect exist correspondence different dosages super-plasticizer: optimal aid dosage, critical supercritical dosage. First, under condition effect, activated had maximum strength little dosage super-plasticizer small on performance cement; second, within similar to common cement, more held...

The paper presents a comparative study of particle size analyses quicklime-stabilized dredged material (LSDM) by combined sieve-hydrometer method (SHM), laser diffraction (LDM) and sieve (SM). After analyzed the testing processes compared results comprehensively, some conclusions can be conduction. Adopting SHM LDM to test LSDM, not only break structure unit soil, but also change its property due pure water used as disperser. In addition, severe flocculation, which attributed dissolvable...

Abstract A rapid and accurate method for the direct simultaneous determination of nitrate nitrite is proposed. The applied to in rainwater wastewater without preliminary separation. determinations are performed by a CPA matrix with ultraviolet spectrophotometric detection. results obtained agreement those conventional methods nitrite.

Abstract This study aimed to optimize the binding process for premade yak steaks using transglutaminase (TG), sodium caseinate, and carrageenan. The optimal ratio of binders conditions were determined by mixture Box–Behnken experimental designs steaks, respectively. Results showed that was as follows: TG 0.31%, caseinate 0.85%, carrageenan 0.34%. tensile strength 844.46 g/s. adhesive content 1.4%, time 10 hr, temperature 12°C. In conclusion, minced meat manufacture improves its utilization,...