A5057264851- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Genetic Mapping and Diversity in Plants and Animals
- Animal Behavior and Welfare Studies
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Effects of Environmental Stressors on Livestock
- Animal Nutrition and Physiology
- Cancer-related molecular mechanisms research
- Meat and Animal Product Quality
- Genetic Syndromes and Imprinting
- Ruminant Nutrition and Digestive Physiology
- Reproductive Biology and Fertility
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Proteoglycans and glycosaminoglycans research
- Muscle Physiology and Disorders
- Pregnancy and preeclampsia studies
- Kruppel-like factors research
- Hypothalamic control of reproductive hormones
- RNA Research and Splicing
- Metallurgy and Material Science
- Genetic diversity and population structure
- Epigenetics and DNA Methylation
- Prion Diseases and Protein Misfolding
Roman L. Hruska U.S. Meat Animal Research Center
2015-2024
Agricultural Research Service
2005-2024
United States Department of Agriculture
2001-2021
University of Nebraska–Lincoln
1997-2020
University of California, Davis
2001-2013
Département Génétique Animale
2001
Utah State University
2001
The University of Melbourne
2001
Commonwealth Scientific and Industrial Research Organisation
2001
Technical University of Munich
2001
A medium-density linkage map of the ovine genome has been developed. Marker data for 550 new loci were generated and merged with previous sheep map. The comprises 1093 markers representing 1062 unique (941 anonymous loci, 121 genes) spans 3500 cM (sex-averaged) autosomes 132 (female) on X chromosome. There is an average spacing 3.4 between autosomal 8.3 highly polymorphic [polymorphic information content (PIC) ≥ 0.7] loci. largest gap 32.5 cM, number gaps >20 or regions where are missing...
A small genetic region near the telomere of ovine chromosome 18 was previously shown to carry mutation causing callipyge muscle hypertrophy phenotype in sheep. Expression this is only known case mammals paternal polar overdominance gene action. surrounding two positional candidate genes sequenced animals genotype. Mutation detection focused on an inbred ram postulated have inherited segments identical-by-descent with exception mutated position. In support hypothesis, homozygous over 210 Kb...
Appendix S1 This annotation presents putative human orthologues to porcine sequences incorporated in the Affymetrix Porcine genome array. The basis and bit score of each entry is provided allow choice an confidence threshold appropriate intended application. Please note: publisher not responsible for content or functionality any supporting information supplied by authors. Any queries (other than missing content) should be directed corresponding author article.
To increase our understanding of imprinted genes in swine, we carried out a comprehensive analysis this gene family using two complementary approaches: expression and phenotypic profiling parthenogenetic fetuses, imprinting by pyrosequencing. The parthenote placenta fetus were smaller than those controls but had no obvious morphological differences at Day 28 gestation. By 30, however, the placentas decreased chorioallantoic folding, chorionic ruggae, reduction fetal-maternal interface...
Placental insufficiency results in fetal loss, low birth weight, stillbirth, preweaning mortality and poor growth. development begins at conceptus elongation, which is a primary factor controlling the size of placenta. After allantois develops outward from embryo to establish allantochorion, defines functional During implantation, chorionic trophoblasts adhere endometrial epithelial cells. structures known as areolae develop openings glands take up gland secreted products (histotrophe)....
An essential component of functional genomics studies is the sequence DNA expressed in tissues interest. To provide a resource bovine-specific data and facilitate this powerful approach cattle research, four normalized cDNA libraries were produced arrayed for high-throughput sequencing. The made with RNA pooled from multiple to increase efficiency normalization maximize number independent genes which obtained. Target included those highest likelihood have impact on production parameters...
The efficiency of nutrient transport from the pregnant female pig to developing fetus depends on size and function placenta. It has been reported that maternal fetal blood vessels are arranged in a cross-countercurrent arrangement within placental microscopic folds. Thus, supplies close apposition each other these folds, flows approximately opposite directions perpendicular plane This indicates width folds influences efficiency. objective this study was determine whether differences fold...
Selection for 11 generations in swine ovulation rate (OR) or uterine capacity (UC) resulted 19.6% greater prenatal survival at term UC compared with OR. Our objective was to characterize the number of fetuses throughout gestation each line, including an unselected control (CO) line. Five hundred ninety-three gilts produced over 4 farrowing seasons were subjected unilateral-hysterectomy-ovariectomy 160 d age and mated within line 280 age. Gilts assigned sire family be slaughtered (+/- 2 d)...
Journal Article Evaluation of the ovine callipyge locus: I. Relative chromosomal position and gene action Get access B. A. Freking, Freking *Roman L. Hruska U.S. Meat Animal Research Center, ARS, USDA, Clay NE 68933, USA†Department Science, University Nebraska-Lincoln 68583-0908 Search for other works by this author on: Oxford Academic PubMed Google Scholar J. W. Keele, Keele USA C. Beattie, Beattie S. M. Kappes, Kappes T.P.L. Smith, Smith T. Sonstegard, Sonstegard K. Nielsen, Nielsen...
Summary Single nucleotide polymorphisms (SNPs) have become an important type of marker for commercial diagnostic and parentage genotyping applications as automated systems been developed that yield accurate genotypes. Unfortunately, allele frequencies public SNP markers in pig populations not available. To fulfil this need, previously mapped the USMARC swine reference population were tested a panel 155 boars representative US purebred Duroc, Hampshire, Landrace Yorkshire populations....
Divergent selection for heat production/loss (kcal.kg-.75.d-1), measured in 9- to 11-wk-old male mice, was conducted 15 generations. Heat loss h on individual animals placed overnight direct, gradient-layer calorimeters. Selection high (MH) and low (ML) unselected control (MC) occurred each of three replicates a total nine unique lines. Repeatability the measurement .45 CV 10.5%. Cumulative realized differentials, averaged replicates, were 145.1 -105.0 (kcal.kg-.75.d-1) ranged from 136.9...
A resource flock of 362 F2 lambs provided phenotypic and genotypic data to estimate effects callipyge (CLPG) genotypes on growth, slaughter, carcass traits. Lambs were serially slaughtered in six groups at 3-wk intervals starting 23 wk age allow comparisons different end points. Probabilities CLPG calculated a position 86 cM from the most centromeric marker chromosome 18. contrast effects, based paternal polar overdominance model, was used evaluate normal phenotypes. Relationships traits...
High-throughput genotyping of swine populations is a potentially efficient method for establishing animal lineage and identification loci important to health pork production. Markers were developed based upon single nucleotide polymorphisms (SNPs), which are abundant amenable automated platforms. The focus this research was SNP discovery in expressed porcine genes providing markers develop the porcine/human comparative map. Locus specific amplification (LSA) sequencing used generate PCR...
Divergent selection for heat loss (kcal·kg−.75·d−1), measured in 9- to 11-wk-old male mice, was conducted 15 generations. Selection high (MH) and low (ML) unselected control (MC) occurred each of three replicates a total nine unique lines. Feed intake males during Generations 9 through 15. Body mass at commencement mating females time measurement recorded. fat percentage 12 wk animals 6, 10, 14 predicted as function electrical conductivity body mass. Litter size recorded all generations,...
The objectives of this study were to estimate effects sire breed (Dorset, Finnsheep, Romanov, Texel, and Montadale), dam (Composite III Northwestern whiteface) on survival, growth, carcass, composition traits F1 lambs. Effects mating season (August, October, December) estimated for survival growth traits. Data collected 4,320 lambs sired by 102 purebred rams over 3 yr. Birth weight was recorded all lambs, subsequent BW adjusted 56 (weaning), 70, 140 d age (n = 3,713, 3,654, 3,579...