A5085925323- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Reproductive Biology and Fertility
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Genomics and Phylogenetic Studies
- Reproductive Physiology in Livestock
- Prenatal Screening and Diagnostics
- Epigenetics and DNA Methylation
- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- Mitochondrial Function and Pathology
- Wheat and Barley Genetics and Pathology
- Molecular Biology Techniques and Applications
- TGF-β signaling in diseases
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Metabolism and Genetic Disorders
- Reproductive System and Pregnancy
- Meat and Animal Product Quality
- Growth Hormone and Insulin-like Growth Factors
- Pregnancy and preeclampsia studies
The University of Adelaide
2015-2024
Ludwig-Maximilians-Universität München
2002-2021
Roman L. Hruska U.S. Meat Animal Research Center
2001-2020
University of New England
2014
South Australia Pathology
2010
Justus-Liebig-Universität Gießen
1996-2006
University of Georgia
2004
University of Guelph
2004
Scientific and Technological Research Council of Turkey
2004
Technical University of Munich
2001-2003
Complete mitochondrial DNA (mtDNA) control regions (CR) were sequenced and analysed in order to investigate wild sheep taxonomy the origin of domestic (Ovis aries). The dataset for phylogenetic analyses includes 63 unique CR sequences from mouflon (O. musimon, O. orientalis), urial vignei), argali ammon) bighorn canadensis) groups, Asia, Europe New Zealand. Domestic occurred two clearly separated branches with musimon) mixed into one clusters. Genetic distances molecular datings based on...
To investigate the origins and phylogenetic relationships of domestic sheep, mitochondrial DNA (mtDNA) from 243 sheep five European, one African, four Asian breeds several mouflon (Ovis musimon), urial (O. vignei bochariensis), argali ammon nigrimontana, O. a. collium) were assayed for restriction fragment length polymorphisms (RFLP). Twenty haplotypes identified which occurred in three major groups: urial/argali, mouflon/domestic, sheep. From branches that contain two lineages are apparent....
A medium-density linkage map of the ovine genome has been developed. Marker data for 550 new loci were generated and merged with previous sheep map. The comprises 1093 markers representing 1062 unique (941 anonymous loci, 121 genes) spans 3500 cM (sex-averaged) autosomes 132 (female) on X chromosome. There is an average spacing 3.4 between autosomal 8.3 highly polymorphic [polymorphic information content (PIC) ≥ 0.7] loci. largest gap 32.5 cM, number gaps >20 or regions where are missing...
Inbred animals were historically chosen for genome analysis to circumvent assembly issues caused by haplotype variation but this resulted in a composite of the two genomes. Here we report haplotype-aware scaffolding and polishing pipeline which was used create haplotype-resolved, chromosome-level assemblies Angus (taurine) Brahman (indicine) cattle subspecies from contigs generated trio binning method. These reveal structural copy number variants that differentiate variant detection is...
Abstract Haplotype-resolved genome assemblies are important for understanding how combinations of variants impact phenotypes. To date, these have been best created with complex protocols, such as cultured cells that contain a single-haplotype (haploid) genome, single where haplotypes separated, or co-sequencing parental genomes in trio-based approach. These approaches impractical most situations. address this issue, we present FALCON-Phase, phasing tool uses ultra-long-range Hi-C chromatin...
Mitochondria have a broad range of functions that affect reproduction, and structural as well quantitative variation in mtDNA has been associated with gamete quality reproductive success. To investigate the mitochondria effect on vitro embryo production, we collected oocytes by ultrasound-guided follicular aspiration from donor cows known to differ developmental capacity, measured blastocyst formation rate, their oocytes. evaluate potential effects mitochondrial function oocyte quality,...
The taurine and zebuine cattle breeds comprise the majority of world population but their taxonomic status is still controversial. two forms are currently classified as <i>Bos taurus </i>and indicus </i>species differentiated primarily by presence or absence a hump. However, these species hybridize readily, producing fully fertile offspring. We have determined analyzed complete <i>B. </i>mitochondrial genome sequences to investigate extent sequence divergences...
The genome of extraembryonic tissue, such as the placenta, is hypomethylated relative to that in somatic tissues. However, origin and role this hypomethylation remains unclear. DNA methyltransferases DNMT1, -3A, -3B are primary mediators establishment maintenance methylation mammals. In study, we investigated promoter methylation-mediated epigenetic down-regulation DNMT genes a potential regulator global levels placental tissue. Although DNMT3A promoters lack all tissues tested, found...
ABSTRACT Haplotype-resolved genome assemblies are important for understanding how combinations of variants impact phenotypes. These can be created in various ways, such as use tissues that contain single-haplotype (haploid) genomes, or by co-sequencing parental but these approaches impractical many situations. We present FALCON-Phase, which integrates long-read sequencing data and ultra-long-range Hi-C chromatin interaction a diploid individual to create high-quality, phased assemblies. The...
To assess the extent of cytoplasmic genetic variability in cloned cattle produced by nuclear transplantation procedures, we investigated 29 individuals seven male clones (sizes 2–6) from two different commercial sources. Restriction enzyme and direct sequence analysis mitochondrial DNA (mtDNA) detected a total 12 haplotypes. Transmitochondrial (i.e., animals which share identical nuclei but have DNA) were all one clones, demonstrating that mtDNA variation among is very common phenomenon...
Epigenetic perturbations are assumed to be responsible for abnormalities observed in fetuses and offspring derived by vitro techniques. We have designed an experiment with bovine Day 80 generated somatic cell nuclear transfer (SCNT), fertilization (IVF), artificial insemination (AI) determine the relationship between fetal phenotype genome-wide 5-methylcytosine (5mC) content. When compared AI controls, SCNT IVF displayed significantly increased body weight (61% 28%), liver (100% 36%), thorax...
Genome scans for quantitative trait loci (QTL) in farm animals have concentrated on primary production and health traits, information QTL other important traits is rare. We performed a whole genome scan granddaughter design to detect affecting body conformation behavior dairy cattle. The analysis included 16 paternal half-sib families of the Holstein breed with 872 sons 264 genetic markers. markers were distributed across all 29 autosomes pseudoautosomal region sex chromosomes average...
We established a short-term (24 h) culture system for bovine oviduct epithelial cells (BOECs), obtained on day 3.5 of the estrous cycle and evaluated with respect to morphological criteria, marker gene expression, hormone responsiveness. BOEC sheets were isolated mechanically from ampulla similar yields oviducts ipsi- contralateral ovulation site (57.9 ± 4.6 56.4 8.0 × 10 6 cells). BOECs showed > 95% purity cultured 24 h maintained characteristics present in vivo , as determined by light...
Varying degrees of mitochondrial DNA (mtDNA) heteroplasmy have been observed in nuclear transfer embryos, fetuses, and offspring, but the mechanisms leading to this condition are unknown. We generated a clone 12 bovine somatic cell using donor cells, recipient oocytes, heifers with defined mtDNA genotypes, study nuclear-mitochondrial interactions origins heteroplasmy. Embryos were reconstructed from granulosa cells Bos taurus type A oocytes collected three different maternal lineages B. B,...
A joint analysis of five paternal half-sib Holstein families that were part two different granddaughter designs (ADR- or Inra-design) was carried out for milk production traits and somatic cell score in order to conduct a QTL confirmation study increase the experimental power. Data exchanged coded standardised form. The combined data set (JOINT-design) consisted on average 231 sires per grandsire. Genetic maps calculated 133 markers distributed over nine chromosomes. analyses performed...
Epigenetic perturbations are assumed to be responsible for phenotypic abnormalities of fetuses and offspring originating from in vitro embryo techniques. We studied 29 viable Day-80 bovine assess the effects two fertilization protocols (IVF1 IVF2) on fetal phenotype genomic cytosine methylation levels liver, skeletal muscle, brain. The IVF1 protocol employed 0.01 U/ml FSH LH oocyte maturation medium 5% estrous cow serum (ECS) culture medium, whereas IVF2 0.2 no 10% ECS culture. Comparisons...