Lignosus rhinocerotis is rich in polysaccharide with diverse -bioactivities. This study developed a pre-column derivatization reversed-phase high-performance liquid chromatography (RP-HPLC) method for analyzing monosaccharides polysaccharides (LRP). LRP underwent hydrolysis, derivatization, and separation on Cosmosil 5C18-MS-II column at 254 nm. Baseline of eight standard was achieved within 45 min. Calibration curves, precision, accuracy were validated. Quantitative analysis revealed as...

Background Chronic hepatitis C virus (HCV) infection is one of the major causes liver cirrhosis and carcinoma. Studies have indicated that an imbalance cytokine activities could contribute to pathogenesis chronic HCV infection. This study aimed investigate serum levels gene expression cytokines (IL-6, TNF-α TGF-β1) in among Malay male subjects. Methods Thirty-nine subjects were enrolled from various health clinics Kelantan, Malaysia, divided into two groups: patients with (HP) healthy...

To investigate the effects of CYP3A4 and CYP2C8 enzymes on repaglinide's pharmacokinetics in healthy Malaysian subjects.Subjects (n = 121) received oral repaglinide (4 mg). Blood samples were taken at 0, 30, 60, 120, 180 240 min serum concentrations determined using high-performance liquid chromatography. Subjects also genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for CYP3A4*4, *5 and*18 an allele-specific multiplex PCR CYP2C8*2, *3, *4...

Pithecellobium jiringa is Malay traditional local delicacy. There no data on antimicrobial nature of P. available. The objective our study was to evaluate the activity jiringa. Leaves, pods and seeds were extracted using methanol. Ten test microorganisms used in study. disc diffusion assay determine sensitivity samples, while liquid dilution method for determination minimal inhibition concentration (MIC). Chloramphenicol as a reference standard. results revealed that all extracts showed...

This study examined the correlation between self-regulated learning and academic achievement among UniSZA undergraduate students. The objective of is to determine impact self-efficacy belief, use strategies on undergraduates’ achievement. A pilot was conducted via exploratory factor analysis using 144 volunteering respondents. reliability validity instrument. closed-ended questionnaire student administered 364 randomly selected students 202 which are female, 162 male across nine faculties...

Colistin is an old antibiotic reintroduced in response to increased incidence of multi-resistant Gram-negative bacterial infections. It has a narrow therapeutic window, and dose optimization required. Dose requires accurate method measurement. The goal this manuscript review previously developed methods, their advantages disadvantages, problems be addressed for colistin assay pharmacokinetic studies. Articles published English from December 2000 2019 were systematically searched using PubMed...

Dopamine receptor and dopamine transporter genes polymorphisms have been associated with cigarette smoking behaviour in different populations. The aim of this case–control study was to evaluate the gene (SLC6A3 (rs27072)) (DRD1 (rs686), DRD2 (rs1800497) DRD3 (rs7653787)) their contribution a Malay male population. We identified 476 participants over age 18 years comprising 238 smokers non-smokers. Information such as age, height, weight, body mass index, systolic diastolic blood pressures,...

Despite modern medicine, there is an increasing trend for cases of the bacterial infection leptospirosis, and this has led to exploration alternative medicines from various sources including plants. The aim study was investigate in vitro anti-leptospiral activity Phyllanthus amarus extracts alone combined with penicillin G, ceftriaxone, doxycycline. Antimicrobial susceptibility testing performed using microdilution broth technique upon methanol extract (ME), aqueous (AE), antibiotics against...

Cytokines play an important role in modulating inflammation during viral infection, including hepatitis C virus (HCV) infection. Genetic polymorphisms of cytokines can alter the immune response against this The objective study was to investigate possible association between chronic infection susceptibility and cytokine gene polymorphism for interleukin-10 (IL-10) rs1800896 rs1800871, interleukin 6 (IL-6) rs1800795, TNF-α rs1800629, TGF-β1 rs1800471 Malay male drug abusers. conducted on 76...

Thalassaemia is a common genetic blood disorder. Knowledge, awareness, attitude towards, and intention to screen for thalassaemia among Malaysians poor. Screening in unmarried individuals plays key role preventing major births.A cross-sectional study was conducted on aged 18 years older Kota Bharu, Kelantan, Malaysia. A questionnaire used collect the general data of participants, their knowledge thalassaemia, towards thalassaemia.A total 278 respondents were included this study. The mean...

Ethnopharmacological relevanceAsthma is a chronic respiratory disorder characterized by inflammation in the airways. The Lignosus rhinocerotis (LR), medicinal fungus containing bioactive compound of polysaccharides, has been employed traditional medicine for centuries to treat various diseases, including asthma. However, there have limited studies on potential polysaccharides isolated from LR managing asthma.Aim studyIn this study, we established an ovalbumin (OVA)-induced mouse model asthma...

The objective of this study was to evaluate the in vitro antibacterial activities methanol and aqueous extracts P. pellucida aerial part (PPAP) against four multi-drug resistant organisms; methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, extended-spectrum beta-lactamase carbapenem-resistant Enterobacteriaceae foodborne pathogens; Bacillus cereus, Escherichia coli Salmonella typhimurium. potentialities plant were evaluated at 250 mg/ml 500 mg/ml. Only...

AbstractColistin, in the form of colistin methanesulfonate sodium (CMS), has a narrow therapeutic window, and levels must be monitored during treatment. The aim this study was to develop validate high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method for measuring human serum. Colistin extracted from serum using trichloroacetic acid methanol protein precipitation, followed by in-solid phase extraction derivatization with 9-fluorenylmethyl chloroformate. HPLC-FLD...

To estimate population pharmacokinetic parameters of repaglinide in 121 healthy Malaysian volunteers.Each subject received 4 mg oral repaglinide. Six blood samples were taken per individual (0, 30, 60, 120, 180 and 240 min) for repaglinide's serum concentration determination by using high-performance liquid chromatography. The parametric Iterative Two-Stage Bayesian Population Model (it2b) program followed the Non-Parametric Adaptive Grid (npag) was used to determine a modelling...

Smoking behavior is influenced by both genetic and environmental factors. Nicotine the major addictive substance in cigarettes. Nicotinic acetylcholine receptors (nAChRs) are thought to play an important role nicotine addiction of smokers. One genes, α-4 subunit nicotinic receptor (CHRNA4) gene was reported be associated with smoking many populations. The aim this study determine association between single nucleotide polymorphism (rs2236196 rs2273502 loci) among Malay Males. conducted...

Objective: The aim of this study was to assess smoking behavior among Malay male smokers in Kelantan, Malaysia.Methods: Volunteers (n=496) were recruited the by randomly selected manner. participants categorized as (n=248) and non-smoking controls (n=248). All given data collection sheets record their information. who group a form containing questionnaires regarding behavior. asked about history such initiation age, factors that influence behavior, number cigarettes daily, quitting attempts,...

This study was conducted to assess the level of counselling competency and knowledge in addiction among counsellors Malaysia Ministry Health. The subjects consisted 50 from Health Johor, Perak, Sabah, Sarawak, Terengganu, Putrajaya. Data were analysed using SPSS version 26. instruments used Malay translated versions Questionnaire Addiction Counseling Curriculum Competencies (ACCC) survey Counselor Scale-Revised (CCS-R) questionnaire. showed that Cronbach’s alpha reliability coefficient for...