Eve Zi Xian Ngoh
A5081384417- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- Long-Term Effects of COVID-19
- Vaccine Coverage and Hesitancy
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Pharmacological Receptor Mechanisms and Effects
- Viral Infections and Immunology Research
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Bacillus and Francisella bacterial research
- COVID-19 Impact on Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Immunodeficiency and Autoimmune Disorders
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Bacterial Infections and Vaccines
Singapore Immunology Network
2020-2024
Agency for Science, Technology and Research
2020-2024
A*STAR Graduate Academy
2022
Abstract Understanding the impact of age on vaccinations is essential for design and delivery vaccines against SARS-CoV-2. Here, we present findings from a comprehensive analysis multiple compartments memory immune response in 312 individuals vaccinated with BNT162b2 SARS-CoV-2 mRNA vaccine. Two vaccine doses induce high antibody T cell responses most individuals. However, recognition Spike protein Delta Omicron variants less efficient than that ancestral Wuhan strain. Age-stratified...
The emergence of new SARS-CoV-2 variants, such as the more transmissible Delta and Omicron has raised concerns on efficacy COVID-19 vaccines. Here, we examined waning antibody responses against different variants following primary booster vaccination. We found that were low response was almost non-existent. Efficient boosting all including Omicron, observed a third dose. tested significantly higher at one month vaccination, compared with two months for individuals, responders identified...
SARS-CoV-2 was first detected in late December 2019, however, the few months that followed, resultant COVID-19 disease has developed into a devastating pandemic around world [[1]https://covid19.who.int/.Google Scholar]. This led to race produce safe and efficacious vaccine record time. In less than year, BNT162b2, an mRNA-based by BioNTech-Pfizer [[2]Polack FP Thomas SJ Kitchin N et al.Safety efficacy of BNT162b2 mRNA Covid-19 vaccine.N Engl J Med. 2020; 383: 2603-2615Crossref PubMed Scopus...
Abstract In vitro antibody selection against pathogens from naïve combinatorial libraries can yield various classes of antigen-specific binders that are distinct those evolved natural infection 1–4 . Also, rapid neutralizing discovery be made possible by a strategy selects for interfering with pathogen and host interaction 5 Here we report the antibodies neutralize SARS-CoV-2, virus responsible COVID-19 pandemic, highly diverse human Fab library. Lead 5A6 blocks receptor binding domain (RBD)...
The rapid spreading of SARS-CoV-2 variants B.1.1.7 originated from the United Kingdom and B.1.351 South Africa has contributed to second wave COVID-19 cases in respective countries also around world. In this study, we employed advanced biochemical virological methodologies evaluate impact Spike mutations these strains on degree protection afforded by humoral immune responses following natural infection ancestral strain during early stages outbreak. We found that antibody-mediated...
In vitro antibody selection against pathogens from naïve combinatorial libraries can yield various classes of antigen-specific binders that are distinct those evolved natural infection. Also, rapid neutralizing discovery be made possible by a strategy selects for interfering with pathogen and host interaction. Here we report the antibodies neutralize SARS-CoV-2, virus responsible COVID-19 pandemic, highly diverse human Fab library. Lead 5A6 blocks receptor binding domain (RBD) viral spike to...
The emergence of escape variants SARS-CoV-2 carrying mutations in the spike protein poses a challenge for therapeutic antibodies. Here, we show that through comprehensive engineering variable region neutralizing monoclonal antibody 5A6, engineered antibody, 5A6CCS1, is able to neutralize escaped neutralization by original 5A6 antibody. In addition improved affinity against variants, 5A6CCS1 was also optimized achieve high solubility and low viscosity, enabling concentration formulation...
Abstract Objective Despite the high vaccine efficacy of mRNA COVID‐19 vaccines, there are individuals who developed excessive reactogenic and/or allergic responses after first dose and were considered ineligible for further doses. CoronaVac, an inactivated SARS‐CoV‐2 vaccine, is recommended in Singapore as alternative. Methods Individuals, vaccines (BNT162b2 or mRNA‐1273) because reactive to prime vaccination, recruited offered two doses CoronaVac booster vaccination 38–224 days post their...
Abstract Waning antibody levels against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and the emergence of variants concern highlight need for booster vaccinations. This is particularly important elderly population, who are at a higher risk developing disease 2019 (COVID‐19) disease. While studies have shown increased responses following vaccination, understanding changes in T B cell compartments induced by third vaccine dose remains limited. We analyzed humoral cellular...
Reliable cell-based platforms to test and/or produce biologics in a sustainable manner are important for the biotech industry. Utilizing enhanced λ integrase, sequence-specific DNA recombinase, we developed novel transgenesis platform involving fully characterized single genomic locus as an artificial landing pad transgene insertion human Expi293F cells. Importantly, instability and variation expression were not observed absence of selection pressure, thus enabling reliable long-term...
Vaccine immunogenicity in transplant recipients can be impacted by the immunosuppressive (IS) regimens they receive. While BNT162b2 vaccination has been shown to induce an immune response liver (LTRs), it remains unclear how different IS may affect vaccine after a third dose LTRs, which is especially important given emergence of Omicron sublineages SARS-CoV-2. A total 95 LTRs receiving single and multiple were recruited offered three doses during study period. Blood samples collected on days...
Identification of the risk factors and high-risk groups which are most vulnerable is critical in COVID-19 disease management at a population level. Evaluating efficacy vaccination against infections necessary to determine booster strategies for better protection groups. In this study, we recruited 158 mRNA-vaccinated individuals during Delta wave SARS-CoV-2 Singapore examined antibody profiles infected individuals. We found that, despite high exposure due communal living conditions...
Abstract Background The emergence of rapidly evolving severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, coupled with waning vaccine-induced immunity, has contributed to the rise vaccine breakthrough infections. It is crucial understand how protection mediated. Methods We examined prospective cohorts mRNA vaccinated and boosted individuals during Omicron wave infection in Singapore. Results found that who remain uninfected over follow-up period had a higher...
Day 1: 1st round bio-panning 1.1Add 2 µM mouse IgG 1 (as Fc blocker) to theHX02 human Fab phage library (Humanyx Pte Ltd) and incubate at RT, 30min. 1.2Wash 60 µl of DynaBeads M-280 Streptavidin (Invitrogen #11205D) with ml PBS twice. 1.3Add 100 nM biotinylated SARS-CoV-2 RBD-mFc protein in casein the beads room temperature for hour. 1.4Wash 4 times PBS. 1.5Add pre-mix washed RBD-mFc. 1.6Mix hour temperature. 1.7Wash 7 1.5 0.1% PBST. Bio-panning Round 1protocols.io|...
Early detection of infections is crucial to limit the spread coronavirus 2019 disease (COVID-19). Here, we developed a flow cytometry-based assay detect SARS-CoV-2 Spike protein (S protein) antibodies in COVID-19 patients. The detected specific IgM and IgG patients also acquisition all subclasses, with IgG1 being most dominant. antibody response was significantly higher at later stage infection. Furthermore, asymptomatic IgG, as dominant subclass. Although levels were lower infections,...
In the current COVID-19 pandemic, understanding establishment and longevity of SARS-CoV-2-specific circulating memory B cell (cMBC) response is essential for maintaining protective immunity. Thirty patients recruited during outbreak in Singapore between January 2020 April were analyzed their cMBC across multiple timepoints up to 12 months post-illness onset. Our data shows that RBD-specific numbers peak at 6 delayed but more persistent with increasing disease severity. responses not...