A5028322937- Genetics, Aging, and Longevity in Model Organisms
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Physiological and biochemical adaptations
- Ion channel regulation and function
- Protein Hydrolysis and Bioactive Peptides
- Endoplasmic Reticulum Stress and Disease
- Force Microscopy Techniques and Applications
- Neuropeptides and Animal Physiology
- Congenital heart defects research
- Reproductive Biology and Fertility
- Digital Holography and Microscopy
- Animal Genetics and Reproduction
- Optical Coherence Tomography Applications
- Retinal Development and Disorders
- Pancreatic function and diabetes
University of Utah
2015-2024
Howard Hughes Medical Institute
2016-2024
Stanford University
2019-2021
Activation of voltage-gated calcium channels at presynaptic terminals leads to local increases in and the fusion synaptic vesicles containing neurotransmitter. Presynaptic output is a function density channels, dynamic properties channel, distance docked vesicles, release probability docking site. We demonstrate that Caenorhabditis elegans neuromuscular junctions two different classes CaV2 CaV1, mediate distinct pools vesicles. are concentrated densely packed clusters ~250 nm diameter with...
Abstract Diverse signaling complexes are precisely assembled at the presynaptic active zone for dynamic modulation of synaptic transmission and plasticity. Presynaptic GABA B -receptors nucleate critical regulating neurotransmitter release most synapses. However, molecular mechanisms underlying assembly -receptor remain unclear. Here we show that neurexins required localization function complexes. At four model synapses, excitatory calyx Held synapses in brainstem, inhibitory on hippocampal...
Highlights•Casein kinase 1 δ (CK1δ) stabilizes nervous system architecture after axon outgrowth•CK1δ phosphorylates and inhibits SSUP-72, an RNA polymerase II CTD phosphatase•CK1δ transcription termination to promote giant Ankyrin expression•Expression of in CK1δ mutants rescues maturation defectsSummaryAfter outgrowth synapse formation, the transitions a stable architecture. In C. elegans, this transition is marked by appearance casein 1δ nucleus. mutants, neurons continue sprout growth...
The C. elegans AWC olfactory neuron pair communicates to specify asymmetric subtypes AWCOFF and AWCON in a stochastic manner. Intercellular communication between other neurons transient NSY-5 gap junction network antagonizes voltage-activated calcium channels, UNC-2 (CaV2) EGL-19 (CaV1), the cell, but how signaling is downregulated by only partly understood. Here, we show that voltage- calcium-activated SLO BK potassium channels mediate inhibit pathways for differentiation. Activation of...
Calcium channels at synaptic boutons are critical for function, but their number and distribution poorly understood. This gap in knowledge is primarily due to the resolution limits of fluorescence microscopy. In last decade, diffraction limit light was surpassed, fluorescent molecules can now be localized with nanometer precision. Concurrently, new gene editing strategies allowed direct tagging endogenous calcium channel genes-expressed correct cells physiological levels. Further,...
Abstract Activation of voltage-gated calcium channels at synapses leads to local increases in and the fusion synaptic vesicles. However, presynaptic output will be determined by density channels, dynamic properties channel, distance docked vesicles, release probability docking site. We demonstrate that C. elegans neuromuscular junctions two different classes CaV2 CaV1, mediate distinct pools are concentrated densely packed clusters ∼300 nm diameter with active zone proteins Neurexin,...
We applied nonlinear optimization to convert a cannula into high-resolution computational-fluorescence microscope. The microscope was used image fluorescent microspheres, genetically-encoded mouse-brain slices, and fluorescent-stained C. elegans nematodes (both living dead animals).
We applied nonlinear optimization to convert a rigid cannula into high-resolution computational-fluorescence microscope. The prototype microscope was used image fluorescent microspheres and genetically-encoded mouse-brain slices produced quality images comparable conventional widefield