A5039257890- Urinary Bladder and Prostate Research
- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Ion Channels and Receptors
- Cardiac electrophysiology and arrhythmias
- Mast cells and histamine
- Circadian rhythm and melatonin
- Receptor Mechanisms and Signaling
- Urological Disorders and Treatments
- Pelvic floor disorders treatments
- Bladder and Urothelial Cancer Treatments
- Neuroscience and Neuropharmacology Research
- Urinary Tract Infections Management
- Thermoregulation and physiological responses
- Cardiovascular Disease and Adiposity
- Blood properties and coagulation
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Tissue Engineering and Regenerative Medicine
- Thermal Regulation in Medicine
- Cardiomyopathy and Myosin Studies
- Neuroscience of respiration and sleep
- Extracellular vesicles in disease
- Renin-Angiotensin System Studies
- Cardiovascular Function and Risk Factors
Michigan State University
2011-2024
Michigan United
2023-2024
University of Vermont
2013-2022
Activation of afferent nerves during urinary bladder (UB) filling conveys the sensation UB fullness to central nervous system (CNS). Although this sensory outflow is presumed reflect graded increases in pressure associated with filling, UBs also exhibit nonvoiding, transient contractions (TCs) that cause small, rapid intravesical pressure. Here, using an ex vivo mouse preparation, we explored relative contributions and TC-induced transients nerve stimulation. Continuous caused increase...
Abstract Severe pain is often experienced by patients with head and neck cancer associated a poor prognosis. Despite its frequency severity, current treatments fail to adequately control cancer-associated because of our lack mechanistic understanding. Although recent works have shed some light the biology underlying in HPV-negative oral cancers, mechanisms mediating HPV+ cancers remain unknown. Cancer-derived small extracellular vesicles (cancer-sEVs) are well positioned function as...
Isolated tissue bath assays are a classical pharmacological tool for evaluating concentration-response relationships in myriad of contractile tissues. While this technique has been implemented over 100 years, the versatility, simplicity and reproducibility assay helps it to remain an indispensable pharmacologists physiologists alike. Tissue systems available wide array shapes sizes, allowing scientist evaluate samples as small murine mesenteric arteries large porcine ileum – if not larger....
Social stress has been implicated as a cause of urinary bladder hypertrophy and dysfunction in humans. Using murine model social stress, we others have shown that leads to overactivity. Here, show overactivity, increased compliance, afferent nerve activity. In the paradigm, 6-wk-old male C57BL/6 mice were exposed for total 2 wk, via barrier cage, retired breeder aggressor mouse. We performed conscious cystometry with without intravesical infusion TRPV1 inhibitor capsazepine, measured...
Isolated tissue bath assays are a classical pharmacological tool for evaluating concentration-response relationships in myriad of contractile tissues. While this technique has been implemented over 100 years, the versatility, simplicity and reproducibility assay helps it to remain an indispensable pharmacologists physiologists alike. Tissue systems available wide array shapes sizes, allowing scientist evaluate samples as small murine mesenteric arteries large porcine ileum – if not larger....
Endothelin receptors (ET<sub>A</sub> and ET<sub>B</sub>) mediate responses to ET-1. ET<sub>B</sub> receptor function seems differ between a similarly sized arterial venous pair, the rat vena cava (RVC) thoracic aorta (RA). RVC contraction directly, but it is unclear whether in RA. Because of these apparent differences receptor-mediated vascular contraction, we hypothesize that relaxant ET<sub>B</sub>-receptor mechanisms would be different from those RA rings were isolated rats for...
KV 7 channels are a family of voltage-dependent K+ expressed in many cell types, which open response to membrane depolarization regulate excitability. Drugs that target used clinically treat epilepsy. Interestingly, these drugs also cause urinary retention, but it was unclear how. In this study, we focused on two possible mechanisms by retigabine could retention: decreasing smooth muscle excitability, or sensory nerve outflow. Urinary bladder had no measurable channel currents. However, the...
A 7-day infusion of serotonin (5-hydroxytryptamine, 5-HT) causes a sustained fall in elevated blood pressure the male deoxycorticosterone acetate (DOCA)-salt rat. As hypertension is long-term disease, we presently test hypothesis that longer (30 day) 5-HT could cause established hypertensive DOCA-salt This time period (∼4 weeks) was also sufficient to whether attenuate development hypertension. (25 μg/kg/min; sc) or vehicle (Veh) delivered via osmotic pump (1) rats for one month, (2)...
Prolonged decreases in urinary bladder blood flow are linked to overactive and underactive pathologies. However, the mechanisms regulating vascular reactivity largely unknown. To investigate these mechanisms, we examined myogenic vasoactive properties of mouse feed arterioles (BFAs). Unlike similar-sized from other beds, BFAs failed constrict response increases intraluminal pressure (5-80 mmHg). Consistent with this lack tone, arteriolar smooth muscle cell membrane potential was...
Social stress causes profound urinary bladder dysfunction in children that often continues into adulthood. We previously discovered the intensity and duration of social influences whether presents as overactivity or underactivity. The transient receptor potential vanilloid type 1 (TRPV1) channel is integral causing stress-induced by increasing sensory outflow, but little known about development sought to determine if TRPV1 channels are involved underactivity caused stress. Voiding function,...
A balance between stiffness and compliance is essential to normal bladder function, changes in the mechanical properties of wall occur many pathologies. These are often associated with release basic secretagogues that turn drive inflammatory mediators from mast cells. Mast cell degranulation by thought activating an orphan receptor, Mas-related G protein-coupled receptor B2 (Mrgprb2). We explored effects putative degranulator Mrgprb2 agonist Compound 48/80 on urinary compliance, smooth...
Transglutaminases (TGs) catalyze the formation of covalent cross-links between glutamine residues and amine groups. This cross-linking activity has been implicated in arterial remodeling. Because hypertension is characterized by remodeling, we hypothesized that TG activity, expression, functionality would be increased aorta, but not vena cava (which does undergo remodeling), from hypertensive rats relative to normotensive rats. Spontaneously stroke-prone (SHRSP) DOCA-salt as well their...
Optimal bladder compliance is essential to urinary storage and voiding functions. Calculated as the change in filling volume per pressure, used clinically characterize changes wall biomechanical properties that associate with lower tract dysfunction. But because this method calculates without regard structure or volume, it gives little insight into mechanical of during filling. Thus, we developed Pentaplanar Reflected Image Macroscopy (PRIM): a novel ex vivo imaging accurately calculate...
Cerebral blood flow is a finely tuned process dependent on coordinated changes in arterial tone. These are strongly tied to smooth muscle membrane potential and inwardly rectifying K + (K IR ) channels thought be key determinant. To elucidate the role of 2.1 cerebral tone development, this study examined electrical functional properties cells, vessels living tissue from tamoxifen-induced cell (SMC)-specific knockout mice. Patch-clamp electrophysiology revealed robust Ba 2+ -sensitive current...
ABSTRACT Benign prostatic hyperplasia/lower urinary tract dysfunction (LUTD) affects nearly all men. Symptoms typically present in the fifth or sixth decade and progressively worsen over remainder of life. Here, we identify a surprising origin this disease that traces back to intrauterine environment developing male, challenging paradigms about when process begins. We delivered single dose widespread environmental contaminant serum most Americans [2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD),...