A5083089403- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Acute Lymphoblastic Leukemia research
- Gene expression and cancer classification
- Chronic Myeloid Leukemia Treatments
- Bioinformatics and Genomic Networks
- Peptidase Inhibition and Analysis
- Metabolism, Diabetes, and Cancer
- Acute Myeloid Leukemia Research
- Adipose Tissue and Metabolism
- Signaling Pathways in Disease
Royal Children's Hospital
2024
Murdoch Children's Research Institute
2024
Walter and Eliza Hall Institute of Medical Research
2018-2022
The University of Melbourne
2021
Fibrosis is driven by the emergence of myofibroblasts, which are primary producers extracellular matrix proteins that form fibrotic lesions. Despite this critical role, detecting myofibroblasts remains challenging due to paucity selective markers. We therefore screened for novel myofibroblast-specific markers, discovering expression close homolog L1 (ChL-1) and embigin (Emb) distinguish activated from their quiescent precursors. report ChL-1+/Emb+ fibroblasts: (1) emerge during cardiac...
Philadelphia-like (Ph-like) acute lymphoblastic leukemia (ALL) is a high-risk subtype of B-cell ALL characterized by gene expression profile resembling Philadelphia chromosome-positive (Ph+ ALL) in the absence BCR-ABL1. Tyrosine kinase-activating fusions, some involving ABL1, are recurrent drivers Ph-like and targetable with tyrosine kinase inhibitors (TKIs). We identified rare instance SFPQ-ABL1 child ALL. expressed cytokine-dependent cell lines was sufficient to transform cells these were...
Type 2 diabetes is preceded by a defective insulin response, yet our knowledge of the precise mechanisms incomplete. Here, we investigate how resistance alters skeletal muscle signaling and exercise partially counteracts this effect. We measured parallel phenotypes phosphoproteomes insulin-resistant (IR) insulin-sensitive (IS) men as they responded to (n = 19, 114 biopsies), quantifying over 12,000 phosphopeptides in each biopsy. Insulin involves selective time-dependent alterations...
Protein phosphorylation is one of the best known post-translational mechanisms playing a key role in regulation cellular processes. Over 100,000 distinct sites have been discovered through constant improvement mass spectrometry based phosphoproteomics last decade. However, data saturation occurring and bottleneck assigning biologically relevant functionality to phosphosites needs be addressed. There has finite success using data-driven approaches reveal phosphosite due range limitations. The...