Cerebrospinal fluid and serum immunoglobulin G from 1007 patients with suspected neurological disease were analysed by 2 methods: isoelectric focusing for the detection of oligoclonal banding, quantitative measurement IgG albumin formulation a Log IgG-Index. A comparison methods in local synthesis showed that not only gave much higher yield overall, 282 showing versus 225 IgG-Index, but also specificity, false positive rate 0% 3.5%. In addition, 163 (58%) Of studied, 206 had multiple...

In this study, a screen-printed electrode (SPE) modified with gold-nanoparticle-decorated reduced graphene oxide-carbon nanotubes (rGO-AuNPs/CNT/SPE) was used for the determination of estradiol (E2). The AuNPs were produced through an eco-friendly method utilising plant extract, eliminating need severe chemicals, and remove requirements sophisticated fabrication methods tedious procedures. addition, rGO-AuNP serves as dispersant CNT to improve dispersion stability CNTs. composite material,...

A revised agarose isoelectric focusing method for detecting oligoclonal IgG in unconcentrated cerebrospinal fluid is presented. The technique shown to be robust and reproducible suitable the detection of intrathecal synthesis.

Propofol is one of the most widely used intravenous drugs for anaesthesia and sedation commonly in intensive care units mechanically ventilated patients. The correct dosage propofol high importance, but there currently a lack suitable point-of-care techniques determining blood concentrations. Here, we present cytochrome P450 2B6/carbon nanotube/graphene oxide/metal oxide nanocomposite sensor discrete measurement concentration. converted into quinol/quinone redox couple by enzyme enables...

Objectives - To investigate the effect of introduction environmental noise, microphone identity & position, and informational noise on accuracy a commercial Clinical AI Scribe (CAIS). Design Consultations five medical conditions (memory loss, diarrhoea, headaches, skin rash, or prostate symptoms) were recorded. A ground-truth audio file was produced for free consultation. This in turn clinical summary via scribe, typically containing 30 facts. Different types 'noise' at different levels...

This paper describes the fabrication of a sensor for 1-hydroxypyrene (1-OHP) based on screen-printed carbon electrode (SPCE) modified with molecularly imprinted polymer (MIP); 1-OHP was chosen as model metabolite polyaromatic hydrocarbons (PAHs). It shown that could be readily oxidised at plain SPCE and electrochemical mechanism found to involve an ECE (electron transfer-chemical reaction-electron transfer) process. The MIP prepared using only divinylbenzene (DVB) styrene monomers binding...

We describe the detection characteristics of a device Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for measurement total prostate specific antigen (PSA) serum samples. A limit 0.8 ng/mL was achieved PSA RCM and is at clinically significant concentrations. Comparison data obtained pilot study from analysis samples with commercially available immunoassays shows good...

The present study assessed the effect of pig genotype (fatty v. lean) and dietary protein lysine (Lys) levels (normal reduced) on intramuscular fat (IMF) content, subcutaneous adipose tissue (SAT) deposition, fatty acid composition mRNA genes controlling lipid metabolism. experiment was conducted sixty intact male pigs (thirty Alentejana purebred thirty Large White × Landrace Pietrain crossbred), from 60 to 93 kg live weight. Animals were divided into three groups fed with following diets:...

The presence of oligoclonal bands (OCBs) immunoglobulin G (IgG) in CSF provides evidence for the occurrence a humoral immune response, but it is not always appreciated that IgG may have originated serum. To determine diagnostic significance serum OCBs 146 patients with were identified among 1874 suspected neurological disorders (7.6%). Clear diagnoses had been made 112 these patients: 56 identical and present, revealing systemic while 46 additional unique indicated intrathecal synthesis was...

By using a benzethonium chloride concentration 12-fold that described originally (Clin Chem 1979;25:1317-9), we developed reliable method suitable for routine measurement of total protein in cerebrospinal fluid. Only 10 microL sample is required. Reactivity to immunoglobulin G (IgG) and albumin (Alb) similar, as necessary specimens can have very varied IgG/Alb ratios. The assay, performed microtiter plate ease use, has between-batch coefficient variation 3.4% 450 mg/L. This contrasts with...

This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors chemical sensors, for indicators mammalian metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including thermometer, onto which sensors (pH, O2) screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells formed over fabricated give 5-well sensor strips interfaced with multipotentiostat via...

The cumulative effects of dietary arginine, leucine and protein levels on fat content, fatty acid composition mRNA genes controlling lipid metabolism in pig longissimus lumborum muscle subcutaneous adipose tissue (SAT) were investigated. experiment was performed fifty-four intact male pigs (Duroc × Pietrain Large White Landrace crossbred), with a live weight ranging from 59 to 92 kg. randomly assigned one six experimental treatments ( n 9). followed 2 3 factorial arrangement, two arginine...

The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using rapid and inexpensive fabrication method utilises colloidal dispersion enhanced sonication. This technique was used to prepare highly ordered uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) 0.5%, 1% 5% concentrations of zinc oxide nano-crystal suspensions. Impedance spectroscopy employed interrogate the confirmed high reproducibility process. Changes in...

In this study, biosensors based on zinc oxide–copper oxide composite nano-surfaces were prepared using a simple and inexpensive distributed colloidal technique. Combinations of mixed dispersions with volume ratios 1:1, 1:2 2:1 ZnO:CuO compared. The uniform nano-crystalline sensor surfaces polyethylene terephthalate (PET) analysed scanning electron microscopy (SEM), Atomic Force Microscopy (AFM) Raman Spectroscopy. ZnO–CuO biosensor showed significantly increased impedimetric signal compared...

Lower yields and poorer quality of biopharmaceutical products result from cell death in bioreactors. Such is commonly associated with programmed or apoptosis. During apoptosis, caspases are activated cause a cascade events that eventually lead to destruction. We report on an impedance spectroscopy measurement technique for the detection total caspase-9 buffer complex fluids, such as culture media. Enhanced sensitivity achieved by leveraging physiochemical properties zinc oxide copper at...

The paper presents a rapid immunoassay system capable of quantifying analyte in complex biological and environmental media. Antibody-coated micrometer-sized paramagnetic particles are used as labels an assay which they bind quantitatively with capture antibody molecules immobilized on polyester disk to form sandwich assay. is performed simple reaction vessel, reactions between labels, analyte, antibodies accelerated by positioning magnets alternately above below the vessel. bound quantified...

Leucocyte zinc concentrations were measured in 70 mothers at the beginning of third trimester pregnancy and compared with weight centiles their subsequently delivered babies. The median maternal leucocyte rose progressively centile. Thus concentration delivering babies weighing below 10th centile was 112 nmol/10(9) leucocytes that above 90th 229.5 leucocytes. A less than 120 strongly predicted a baby (positive predictive value = 71.9%, negative 91.5%, sensitivity 64.3%, specificity 81.8%)....