A5039251963- Enzyme Catalysis and Immobilization
- Genomics and Phylogenetic Studies
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Distributed and Parallel Computing Systems
- RNA and protein synthesis mechanisms
- Advanced Data Storage Technologies
- Scientific Computing and Data Management
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Bioeconomy and Sustainability Development
- Methane Hydrates and Related Phenomena
- Wastewater Treatment and Nitrogen Removal
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Chemical Reactions and Isotopes
- Pancreatic function and diabetes
- Steroid Chemistry and Biochemistry
- Nanomaterials for catalytic reactions
- Oil, Gas, and Environmental Issues
- Metalloenzymes and iron-sulfur proteins
- Genetically Modified Organisms Research
- Cassava research and cyanide
Kurchatov Institute
2015-2024
Genetika
2004-2019
Abstract Two new bacterial strains, Pseudomonas marginales MA32 and putida MA113, containing nitrile hydratases resistant to cyanide were isolated from soil samples by an enrichment procedure. In contrast known hydratases, which rapidly lose activity at low moderate concentrations, the enzymes described in this paper tolerate up 50 mM cyanide. They show a broad substrate spectrum including not only small substrates like acrylonitrile but also nitriles with longer side chains even quarternary...
Abstract This study highlights the effect of heavy metal ions on expression cobalt-containing nitrile hydratase (NHase) in Rhodococcus strains, which over-produce this enzyme. Both metal-dependent derepression transcription and maturation NHase were considered. We demonstrated that nickel can derepress promoter several strains. The cblA gene a cobalt-dependent transcriptional repressor was shown to be indispensable for nickel-mediated derepression. As maturation, we showed could not replace...
Rhodococcus bacteria are a promising platform for biodegradation, biocatalysis, and biosynthesis, but the use of rhodococci is hampered by insufficient number both strains expression promoters that functional thoroughly studied in these strains. To expand list such promoters, we capability rhodochrous M33 strain, functioning set recombinant it. We showed strain supports superexpression target enzyme (nitrile hydratase) using alternative inexpensive feedings-acetate urea-without growth factor...
Rhodococcus sp. strain M8 is a nitrile-degrading bacterium isolated from acrylonitrile-contaminated sites. This produces the enzymes for sequential nitrile degradation, cobalt-type hydratase, and amidase in large amounts. Its draft genome sequence, announced here, has an estimated size of 6.3 Mbp.
All Most of the remote sensing applications rely on daytime visible and infrared images Earth surface. Increase in number satellites, their spatial resolution as well simultaneously observed spectral bands ensure a steady growth data volumes computational complexity sciences. Recent advance night time is related to enhanced sensitivity on-board instruments unique opportunity observe "pure" emitters spectra without contamination from solar heat reflected light. A candidate set night-time...
We describe here a 6.6-Mb draft genome sequence of Rhodococcus erythropolis strain HX7, which was obtained from soil samples collected the northern Arkhangelsk region in Russian Federation. This genomic resource will support further study mechanisms cold-resistant oil degradation and potentially aid bioremediation cold oil-producing regions.
We report a 6.27-Mbp complete genome of Rhodococcus sp. strain M8, an originally discovered that is now under investigation for production acrylic monomers. The consists 6.1-Mbp circular chromosome and 173.2-kbp plasmid.