A5012734199- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Metalloenzymes and iron-sulfur proteins
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Chemical Reactions and Isotopes
- Biofuel production and bioconversion
- Enzyme function and inhibition
- Bacterial Genetics and Biotechnology
- Algal biology and biofuel production
- Advanced Fluorescence Microscopy Techniques
- Nanomaterials for catalytic reactions
Kurchatov Institute
2019-2024
Genetika
2018-2019
Abstract This study highlights the effect of heavy metal ions on expression cobalt-containing nitrile hydratase (NHase) in Rhodococcus strains, which over-produce this enzyme. Both metal-dependent derepression transcription and maturation NHase were considered. We demonstrated that nickel can derepress promoter several strains. The cblA gene a cobalt-dependent transcriptional repressor was shown to be indispensable for nickel-mediated derepression. As maturation, we showed could not replace...
Rhodococcus bacteria are a promising platform for biodegradation, biocatalysis, and biosynthesis, but the use of rhodococci is hampered by insufficient number both strains expression promoters that functional thoroughly studied in these strains. To expand list such promoters, we capability rhodochrous M33 strain, functioning set recombinant it. We showed strain supports superexpression target enzyme (nitrile hydratase) using alternative inexpensive feedings-acetate urea-without growth factor...
The Rhodococcus rhodochrous M33-2nit strain containing in its chromosome two copies of a gene for A. denitrificans В-9582 NitC1 nitrilase under the control promoter region genes R. M8 nitrile hydratase has been constructed. culturing was optimized and it shown that using two-substrate cultivation scheme on glucose acetate, possible to obtain up 17 g cdw/L cells with specific activity 7 units/mg cdw. A capacity synthesizing ammonium acrylate from acrylonitrile by conditions simulating...
Rhodococcus strains were designed as model biocatalysts (BCs) for the production of acrylic acid and mixtures monomers consisting acrylamide, acid, N-alkylacrylamide (N-isopropylacrylamide). To obtain BC strains, we used, among other approaches, adaptive laboratory evolution (ALE), based on use metabolic pathway amide utilization. Whole genome sequencing obtained after ALE, well subsequent targeted gene disruption, identified candidate genes three new amidases that are promising development...