A5101660618- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- Connective tissue disorders research
- Lipoproteins and Cardiovascular Health
- Animal Genetics and Reproduction
- interferon and immune responses
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- NF-κB Signaling Pathways
- CAR-T cell therapy research
- Lipid metabolism and disorders
- Mitochondrial Function and Pathology
Temple University
2020
Gansu Provincial Hospital of TCM
2019
Affiliated Hospital of Jining Medical University
2018
Tongji University
2016-2017
Tongji Hospital
2016-2017
Guangdong General Hospital
2017
Chengdu Military General Hospital
2012-2014
Nankai University
2006
Abstract MicroRNAs (miRNAs) have been reported to play critical roles in the occurrence, progression, and treatment of many cardiovascular diseases. However, molecular mechanism by which miRNA regulates target gene expression ischemia‐reperfusion (I/R) injury acute myocardial infarction (AMI) is not entirely clear. MiR‐340‐5p was be downregulated ischemic stroke. it still remains unknown whether miR‐340‐5p mediated pathogenesis process I/R after AMI. In present study, male C57BL/6 J mice...
Recombinant adeno-associated viral (rAAV) vectors have been widely used in human gene therapy. One major impediment to its broad application is the inability produce high-quality mass quantity. Here, an efficient and scalable suspension cell culture system for production of rAAV described. In this system, AAV trans factors, Rep78, Rep52, VP1, VP2, VP3, were stably integrated into a single vaccinia virus carrier by maximizing use alternative codons between genes with identical amino acids,...
The long non‑coding RNA (lncRNA), urothelial carcinoma‑associated 1 (UCA1), has been demonstrated to be dysregulated and serves a role in the progression of several cancer types. However, exact effects underlying molecular mechanisms UCA1 anaplastic thyroid (ATC) remain unknown. aim present study was investigate detailed function mechanism regulation ATC cell progression. identified that expression levels UCA1, lines tissues, were significantly upregulated compared with normal human line...
Although microRNA-1 ( miR-1 ) is a known liver cancer suppressor, the role of in apoptosis hepatoma cells has remained largely unknown. Our study shows that ectopic overexpression induced hepatocellular carcinoma (HepG2) cells. Apoptosis inhibitor 5 API-5 was found to be potential regulator apoptosis, using bioinformatics approach. Furthermore, an inverse relationship between and expression observed human tissues adjacent normal tissues. Negative regulation by demonstrated promote HepG2...
One important limitation for achieving therapeutic expression of human factor VIII (FVIII) in hemophilia A gene therapy is inefficient secretion the FVIII protein. Substitution five amino acids A1 domain with corresponding porcine residues generated a secretion-enhanced variant termed B-domain-deleted (BDD)-FVIII-X5 that resulted 8-fold higher activity levels supernatant an vitro cell-based assay system than seen unmodified BDD-FVIII. Analysis purified recombinant BDD-FVIII-X5 and BDD-FVIII...
Objective To investigate the effect of cyclic-tension force on expression osteogenesis genes in human periodontal ligament cells (HPDLC). Methods HPDLC were cultured flexible-bottomed plates and subjected to 12% elongation by strain unit at 6 cycles/min (i.e.5-s 5-s relaxation) for 48 hours experimental groups. GEArray Q series Human Osteogenesis Gene Array was used identify expressed HPDLC, including growth factors associated molecules, extracellular matrix its proteins, cell adhesion...
Objective To screen the most effective short hairpin RNA (shRNA) which down-regulate insulin-like growth factor binding protein 3 (IGFBP-3) in corpus cavernosal smooth muscle cell (CCSMC) vitro. Methods 4 pair IGFBP-3 small interfering (siRNA), and plasmid constructs for U6 promoter vector (pGPU6/GFP/Neo-IGFBP-3-shRNA) negative control were constructed. Corpus cells primary cultured divided into 6 groups: CCSMCs-only, transfection pGPU6/GFP/Neo-IGFBP-3 (611) group, (526) (866) (710)...