A5076624873- Microbial metabolism and enzyme function
- Microbial bioremediation and biosurfactants
- Chromium effects and bioremediation
- Water Treatment and Disinfection
- Biofuel production and bioconversion
- Geochemistry and Elemental Analysis
- RNA and protein synthesis mechanisms
- Anaerobic Digestion and Biogas Production
- Radioactive element chemistry and processing
- Bacterial Genetics and Biotechnology
- Mercury impact and mitigation studies
- Extraction and Separation Processes
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- Lanthanide and Transition Metal Complexes
- Corrosion Behavior and Inhibition
- Metalloenzymes and iron-sulfur proteins
- Metal-Catalyzed Oxygenation Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Trace Elements in Health
Lawrence Livermore National Laboratory
2023-2024
University of Michigan–Ann Arbor
2020-2023
Abstract Technologically critical rare-earth elements are notoriously difficult to separate, owing their subtle differences in ionic radius and coordination number 1–3 . The natural lanthanide-binding protein lanmodulin (LanM) 4,5 is a sustainable alternative conventional solvent-extraction-based separation 6 Here we characterize new LanM, from Hansschlegelia quercus ( Hans -LanM), with an oligomeric state sensitive radius, the lanthanum(III)-induced dimer being >100-fold tighter than...
Chemical methods for the extraction and refinement of technologically critical rare earth elements (REEs) are energy-intensive, hazardous, environmentally destructive. Current biobased systems rely on extremophilic organisms generate many same detrimental effects as chemical methodologies. The mesophilic methylotrophic bacterium Methylobacterium extorquens AM1 was previously shown to grow using electronic waste by naturally acquiring REEs power methanol metabolism. Here we show that growth a...
Copper is an important component of methanotrophic physiology, as it controls the expression and activity alternative forms methane monooxygenase (MMO). To collect copper, some methanotrophs secrete a chalkophore- or copper-binding compound called methanobactin (MB). MB ribosomally synthesized posttranslationally modified polypeptide (RiPP) that, after binding collected by MbnT, TonB-dependent transporter (TBDT). Structurally different have been characterized, here, we show that are specific...
ABSTRACT Chemical methods for extraction and refinement of technologically critical rare earth elements (REEs) are energy intensive, hazardous, environmentally destructive. Current bio-based systems rely on extremophilic organisms generate many the same detrimental effects as chemical methodologies. The mesophilic methylotrophic bacterium Methylobacterium extorquens AM1 was previously shown to grow using electronic waste by naturally acquiring REEs power methanol metabolism. Here we show...
Methanobactins (MBs) are ribosomally synthesized and posttranslationally modified peptides (RiPPs) produced by methanotrophs for copper uptake. The posttranslational modification that defines MBs is the formation of two heterocyclic groups with associated thioamines from X-Cys dipeptide sequences. Both in MB Methylosinus trichosporium OB3b (MB-OB3b) oxazolone groups. precursor gene MB-OB3b mbnA, which part a cluster contains both annotated unannotated genes. One those genes, mbnC, found all...
The design of two overlapping genes in a microbial genome is an emerging technique for adding more reliable control mechanisms engineered organisms increased stability. functional gene pairs challenging procedure, and computational tools are used to improve the efficiency deploy successful designs genetically systems. GENTANGLE (Gene Tuples ArraNGed overLapping Elements) high-performance containerized pipeline translated different reading frames genome. This new software package can be test...
The genomes of Methylomonas sp. strain EFPC1 and Methylococcus EFPC2 isolated from a mercury-contaminated stream in Oak Ridge Tennessee, were sequenced.
Abstract Summary The design of two overlapping genes in a microbial genome is an emerging technique for adding more reliable control mechanisms engineered organisms increased safety. functional gene pairs challenging procedure and computational tools are used to improve the efficiency deploy successful designs genetically systems. GENTANGLE (Gene Tuples ArraNGed overLapping Elements) high performance containerized pipeline translated different reading frames genome. This new software package...
The genome of