A5052960048- Microbial metabolism and enzyme function
- Metalloenzymes and iron-sulfur proteins
- Microbial Fuel Cells and Bioremediation
- Electrocatalysts for Energy Conversion
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial bioremediation and biosurfactants
- Anaerobic Digestion and Biogas Production
- Atmospheric and Environmental Gas Dynamics
- Ammonia Synthesis and Nitrogen Reduction
- Membrane-based Ion Separation Techniques
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Porphyrin Metabolism and Disorders
- Petroleum Processing and Analysis
- Wastewater Treatment and Nitrogen Removal
- Carbon Dioxide Capture Technologies
- Hydrocarbon exploration and reservoir analysis
- Microbial Community Ecology and Physiology
- Supercapacitor Materials and Fabrication
- Chromium effects and bioremediation
- Supramolecular Self-Assembly in Materials
- Methane Hydrates and Related Phenomena
- Corrosion Behavior and Inhibition
- CO2 Reduction Techniques and Catalysts
- Biosensors and Analytical Detection
École Polytechnique Fédérale de Lausanne
2023-2024
Hainan University
2024
Industrial Microbes (United States)
2024
Biocatalysts (United Kingdom)
2024
Cascade (United States)
2024
Stanford University
2020-2023
University of Michigan–Ann Arbor
2015-2022
Johns Hopkins University
2013-2022
Stanford Medicine
2020-2021
Methylmercury (CH3Hg+) is a potent neurotoxin produced by certain anaerobic microorganisms in natural environments. Although numerous studies have characterized the basis of mercury (Hg) methylation, no examined CH3Hg+ degradation methanotrophs, despite their ubiquitous presence environment. We report that some such as Methylosinus trichosporium OB3b, can take up and degrade rapidly, whereas others, Methylococcus capsulatus Bath, but not CH3Hg+. Demethylation M. OB3b increases with...
It is well known that Methylosinus trichosporium OB3b has two forms of methane monooxygenase (MMO) responsible for the initial conversion to methanol, a cytoplasmic (soluble) and membrane-associated (particulate) monooxygenase, copper strongly regulates expression these alternative MMO. More recently, it been discovered M. multiple types methanol dehydrogenase (MeDH), i.e. Mxa-type MeDH (Mxa-MeDH) Xox-type (Xox-MeDH), regulated by availability rare earth element (REE), cerium. Here, we...
A crewed mission to and from Mars may include an exciting array of enabling biotechnologies that leverage inherent mass, power, volume advantages over traditional abiotic approaches. In this perspective, we articulate the scientific engineering goals constraints, along with example systems, guide design a surface biomanufactory. Extending past arguments for exploiting stand-alone elements biology, argue integrated biomanufacturing plant replete modules microbial in situ resource utilization,...
Directly integrated system demonstrating robust microbial electromethanogenesis at high rate and efficiency without requiring adaptation to electrochemical conditions.
Microbial electrosynthesis (MES) of acetate is a process using electrical energy to reduce CO2 acetic acid in an integrated bioelectrochemical system. MES powered by excess renewable electricity produces carbon-neutral while benefitting from inexpensive but intermittent sources. Interruptions supply also cause limitation and starvation the microbial cells performing MES. Here, we studied effect on performance hydrogen-mediated acetate. Thermoanaerobacter kivui produced for more than 4 months...
Most microorganisms in nature spend the majority of time a state slow or zero growth and metabolism under limited energy nutrient flux rather than growing at maximum rates. Yet, most our knowledge has been derived from studies on fast-growing bacteria. Here, we systematically characterized physiology methanogenic archaeon Methanococcus maripaludis during growth. M. was grown continuous culture (formate)-limiting conditions different dilution rates ranging 0.09 to 0.002 h-1, latter...
Abstract Aerobic methanotrophs are a specialized microbial group, catalyzing the oxidation of methane. Disturbance-induced loss methanotroph diversity/abundance, thus results in this biological methane sink. Here, we synthesized and conceptualized resilience to sporadic, recurring, compounded disturbances soils. The showed remarkable sporadic disturbances, recovering activity population size. However, was severely compromised when disturbance persisted or reoccurred at increasing frequency,...
ABSTRACT Methanobactin, a small modified polypeptide synthesized by methanotrophs for copper uptake, has been found to be chromosomally encoded. The gene encoding the precursor of methanobactin, mbnA , is part cluster that also includes several genes proteins unknown function (but speculated involved in methanobactin formation) as well mbnT which encodes TonB-dependent transporter hypothesized responsible uptake. To determine if truly knockout was constructed Methylosinus trichosporium OB3b...
Abstract Molecular hydrogen is a major high‐energy carrier for future energy technologies, if produced from renewable electrical energy. Hydrogenase enzymes offer pathway bioelectrochemically producing that advantageous over traditional platforms production because of low overpotentials and ambient operating temperature pressure. However, electron delivery the electrode surface to enzyme's active site often rate‐limiting. Here, it shown three different hydrogenases Clostridium pasteurianum...
Methanotrophs or methane-oxidizing bacteria exhibit a unique ‘copper-switch’ where expression of two forms methane monooxygenase (MMO) is controlled by the availability copper. In absence copper, cytoplasmic soluble (sMMO) expressed. presence membrane-bound particulate (pMMO) These MMO have very different properties, and elucidation basis copper-switch significant interest as methanotrophs are becoming increasingly popular for valorization methane. Recently, it was suggested via...
Proteobacterial methanotrophs—groups of microorganisms that utilize methane as a source energy and carbon—have been known to unique mechanisms scavenge copper, namely, utilization methanobactin, polypeptide binds copper with high affinity specificity. Previously the possibility sequestration by methanotrophs may lead alteration cuproenzyme-mediated reactions in denitrifiers consequently increase emission potent greenhouse gas N 2 O has suggested axenic coculture experiments.
ABSTRACT Gene expression in methanotrophs has been shown to be affected by the availability of a variety metals, most notably copper-regulating alternative forms methane monooxygenase. A copper-binding compound, or chalkophore, called methanobactin plays key role copper uptake methanotrophs. Methanobactin is ribosomally synthesized and posttranslationally modified peptide (RiPP) with two heterocyclic rings an associated thioamide for each ring, formed from X-Cys dipeptide sequences that bind...
As the only enzyme currently known to reduce dinitrogen (N2) ammonia (NH3), nitrogenase is of significant interest for bio-inspired catalyst design and new biotechnologies aiming produce NH3 from N2. In order N2, must also hydrolyze at least 16 equivalents adenosine triphosphate (MgATP), representing consumption a quantity energy available biological systems. Here, we review natural engineered electron transfer pathways nitrogenase, including strategies redirect or redistribute flow in vivo...
ABSTRACT Methanotrophs have remarkable redundancy in multiple steps of the central pathway methane oxidation to carbon dioxide. For example, it has been known for over 30 years that two forms monooxygenase, responsible oxidizing methanol, exist methanotrophs, i.e., soluble monooxygenase (sMMO) and particulate (pMMO), expression these is controlled by availability copper. Specifically, sMMO occurs absence copper, while pMMO increases with increasing copper concentrations. More recently, was...
The substrate-reducing proteins of all nitrogenases (MoFe, VFe, and FeFe) are organized as α2ß2(γ2) multimers with two functional halves. While their dimeric organization could afford improved structural stability in vivo, previous research has proposed both negative positive cooperativity contributions respect to enzymatic activity. Here, a 1.4 kDa peptide was covalently introduced the proximity P cluster, corresponding Fe protein docking position. Strep-tag carried by added simultaneously...
CH 4 emissions have contributed ~30% of global warming to date [1], and natural sources may increase via
The nitrogenase reductase NifH catalyses ATP‐dependent electron delivery to the Mo‐nitrogenase, a reaction central biological dinitrogen (N 2 ) fixation. While NifHs have been extensively studied in bacteria, structural information about their archaeal counterparts is limited. Archaeal are considered more ancient, particularly those from Methanococcales , group of marine hydrogenotrophic methanogens, which includes diazotrophs growing at temperatures near 92 °C. Here, we structurally and...
The biological N2-fixation process is catalyzed exclusively by metallocofactor-containing nitrogenases. Structural and spectroscopic studies highlighted the presence of an additional mononuclear metal-binding (MMB) site, which can coordinate Fe in addition to two metallocofactors required for reaction. This MMB site located 15-Å from active at interface NifK subunits. enigmatic function its implications metallocofactor installation, catalysis, electron transfer, or structural stability are...