A5084027204- Microbial metabolism and enzyme function
- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Microbial bioremediation and biosurfactants
- Anaerobic Digestion and Biogas Production
- Wastewater Treatment and Nitrogen Removal
- Atmospheric and Environmental Gas Dynamics
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Hydrocarbon exploration and reservoir analysis
- Metalloenzymes and iron-sulfur proteins
- Polyomavirus and related diseases
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Odor and Emission Control Technologies
- Full-Duplex Wireless Communications
- Porphyrin Metabolism and Disorders
- Pharmaceutical and Antibiotic Environmental Impacts
- Energy Harvesting in Wireless Networks
- Protist diversity and phylogeny
- Antenna Design and Analysis
- Coal Properties and Utilization
- Polar Research and Ecology
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
University of the Punjab
2020-2024
Daffodil International University
2022
University of East Anglia
2017-2022
Riphah International University
2021
Lahore College for Women University
2021
Norwich Research Park
2018
University of Michigan
2014-2017
Centre National de la Recherche Scientifique
2011-2017
Université de Strasbourg
2011-2017
Génétique Moléculaire Génomique Microbiologie
2013-2017
Methylmercury (CH3Hg+) is a potent neurotoxin produced by certain anaerobic microorganisms in natural environments. Although numerous studies have characterized the basis of mercury (Hg) methylation, no examined CH3Hg+ degradation methanotrophs, despite their ubiquitous presence environment. We report that some such as Methylosinus trichosporium OB3b, can take up and degrade rapidly, whereas others, Methylococcus capsulatus Bath, but not CH3Hg+. Demethylation M. OB3b increases with...
The complete and assembled genome sequences were determined for six strains of the alphaproteobacterial genus Methylobacterium, chosen their key adaptations to different plant-associated niches environmental constraints.
Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a "copper switch." At low copper/biomass ratios the soluble monooxygenase (sMMO) is expressed while expression and activity of particulate (pMMO) increases with increasing availability copper. In many methanotrophs there also methanol dehydrogenases (MeDHs), one based on Mxa another Xox. Mxa-MeDH calcium in its active site, Xox-MeDHs been shown rare earth elements their site. We show here...
It is well known that Methylosinus trichosporium OB3b has two forms of methane monooxygenase (MMO) responsible for the initial conversion to methanol, a cytoplasmic (soluble) and membrane-associated (particulate) monooxygenase, copper strongly regulates expression these alternative MMO. More recently, it been discovered M. multiple types methanol dehydrogenase (MeDH), i.e. Mxa-type MeDH (Mxa-MeDH) Xox-type (Xox-MeDH), regulated by availability rare earth element (REE), cerium. Here, we...
Chloromethane gas is produced naturally in the phyllosphere, compartment defined as aboveground parts of vegetation, which hosts a rich bacterial flora. may serve growth substrate for specialized aerobic methylotrophic bacteria, have been isolated from soil and water environments, use cmu genes chloromethane utilization. Evidence presence chloromethane-degrading bacteria on leaf surfaces Arabidopsis thaliana was obtained by specific quantitative PCR cmuA gene encoding two-domain...
ABSTRACT Methanobactin, a small modified polypeptide synthesized by methanotrophs for copper uptake, has been found to be chromosomally encoded. The gene encoding the precursor of methanobactin, mbnA , is part cluster that also includes several genes proteins unknown function (but speculated involved in methanobactin formation) as well mbnT which encodes TonB-dependent transporter hypothesized responsible uptake. To determine if truly knockout was constructed Methylosinus trichosporium OB3b...
Anaerobic ammonium oxidation (anammox) is a major process of bioavailable nitrogen removal from marine systems. Previously, bacteriohopanetetrol (BHT) isomer, with unknown stereochemistry, eluting later than BHT when examined by high performance liquid chromatography (HPLC), was detected in ‘Ca. Scalindua profunda’ and proposed as biomarker for anammox paleo-environments. However, the utility this isomer an hindered fact that four other, non-anammox, bacteria are also known to produce...
Abstract Background Natural gas seeps contribute to global climate change by releasing substantial amounts of the potent greenhouse methane and other climate-active gases including ethane propane atmosphere. However, methanotrophs, bacteria capable utilising as sole source carbon energy, play a significant role in reducing emissions from many environments. Methylocella -like facultative methanotrophs are unique group that grow on components natural (i.e. propane) addition but little is known...
Methanotrophs or methane-oxidizing bacteria exhibit a unique ‘copper-switch’ where expression of two forms methane monooxygenase (MMO) is controlled by the availability copper. In absence copper, cytoplasmic soluble (sMMO) expressed. presence membrane-bound particulate (pMMO) These MMO have very different properties, and elucidation basis copper-switch significant interest as methanotrophs are becoming increasingly popular for valorization methane. Recently, it was suggested via...
Natural gas contains methane and the gaseous alkanes ethane, propane butane, which collectively influence atmospheric chemistry cause global warming. Methane-oxidising bacteria, methanotrophs, are crucial in mitigating emissions of as they oxidise most produced soils subsurface before it reaches atmosphere. Methanotrophs usually obligate, i.e. grow only on not longer chain alkanes. Bacteria that other natural such have also been characterised, but do methane. Recently, was shown facultative...
Hyphomicrobium sp. strain MC1 is an aerobic methylotroph originally isolated from industrial sewage. This prosthecate bacterium was the first reported to grow with chloromethane as sole carbon and energy source. Its genome, consisting of a single 4.76-Mb chromosome, for chloromethane-degrading be formally reported.
ABSTRACT It is well known that copper a key factor regulating expression of the two forms methane monooxygenase found in proteobacterial methanotrophs. Of these forms, cytoplasmic, or soluble, (sMMO) expressed only at low concentrations. The membrane-bound, particulate, (pMMO) constitutively with respect to copper, and such increases increasing copper. Recent findings have shown uptake mediated by modified polypeptide, chalkophore, termed methanobactin. Although methanobactin has high...
Methanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) (pMMO). Expression of these MMOs is strongly regulated by the availability copper. Many methanotrophs have been found to synthesize novel compound, methanobactin (Mb), that responsible for uptake copper, and produced Methylosinus trichosporium OB3b plays key role in controlling expression MMO genes this strain. As all known forms are structurally similar, it was hypothesized from...
Approximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar that methane, and despite its significant effects on climate, very little is known about biological degradation in environment. Isolation characterisation degraders at molecular level has allowed development probes targeting isoA encoding α-subunit monooxygenase. This enzyme belongs soluble diiron centre monooxygenase family catalyses first step pathway. The use key metabolic genes a successful...
Methanethiol (MeSH) and dimethylsulfide (DMS) are volatile organic sulfur compounds (VOSCs) with important roles in cycling, signaling atmospheric chemistry. DMS can be produced from MeSH through a reaction mediated by the methyltransferase MddA. The mddA gene is present terrestrial marine metagenomes, being most abundant soil environments. substrate for MddA, MeSH, also oxidized bacteria oxidase (MTO) enzyme, encoded mtoX gene, found marine, freshwater metagenomes. Methanethiol-dependent...
Plant-produced isoprene (2-methyl-1,3-butadiene) represents a significant portion of global volatile organic compound production, equaled only by methane. A metabolic pathway for the degradation was first described Gram-positive bacterium Rhodococcus sp. AD45, and an alternative model organism has yet to be characterised. Here, we report characterisation novel Gram-negative isoprene-degrading bacterium, Variovorax WS11. Isoprene metabolism in this involves plasmid-encoded iso gene cluster...
Abstract Natural gas seeps release significant amounts of methane and other gases including ethane propane contributing to global climate change. In this study, bacterial actively consuming short-chain alkanes were identified by cultivation, whole-genome sequencing, stable-isotope probing (SIP)-metagenomics using 13C-propane 13C-ethane from two different natural seeps, Pipe Creek Andreiasu Everlasting Fire. Nearly 100 metagenome-assembled genomes (MAGs) (completeness 70–99%) recovered both...
ABSTRACT Methanotrophs have remarkable redundancy in multiple steps of the central pathway methane oxidation to carbon dioxide. For example, it has been known for over 30 years that two forms monooxygenase, responsible oxidizing methanol, exist methanotrophs, i.e., soluble monooxygenase (sMMO) and particulate (pMMO), expression these is controlled by availability copper. Specifically, sMMO occurs absence copper, while pMMO increases with increasing copper concentrations. More recently, was...