The climate-active gas isoprene (2-methyl-1,3-butadiene) is released to the atmosphere in huge quantities, almost equaling that of methane, yet we know little about biological cycling environment. Although bacteria capable growth on as sole source carbon and energy have previously been isolated from soils sediments, no microbiological studies targeted major examined phyllosphere isoprene-emitting trees for presence degraders this abundant source. Here, identified isoprene-degrading poplar...

Abstract Carbon monoxide (CO) oxidising microorganisms are present in volcanic deposits throughout succession, with levels of vegetation and soil influencing the communities present. Carboxydovores a subset CO oxidisers that use as an energy source, which raises questions about physiological metabolic features make them more competitive harsh ecosystems. To address these questions, samples were taken from strata formed by eruptions Calbuco Volcano (Chile) 2015 (tephra) 1917 (soil). Two...

Outer-membrane vesicles (OMVs) produced by Helicobacter pylori deliver bacterial components to host cells, provide a mechanism for stabilization of secreted and may allow the bacteria exert 'long-range' effects in gastric niche, promoting persistence. In addition their well-characterized cell interactions, membrane improve stress survival other species, are constitutively both pathogenic non-pathogenic bacteria. We aimed determine whether OMVs could H. range stressors. The purified on...

Plant-produced isoprene (2-methyl-1,3-butadiene) represents a significant portion of global volatile organic compound production, equaled only by methane. A metabolic pathway for the degradation was first described Gram-positive bacterium Rhodococcus sp. AD45, and an alternative model organism has yet to be characterised. Here, we report characterisation novel Gram-negative isoprene-degrading bacterium, Variovorax WS11. Isoprene metabolism in this involves plasmid-encoded iso gene cluster...

Isoprene (2-methyl-1,3-butadiene) is emitted in vast quantities (>500 Tg C yr -1 ). Most isoprene by trees, but there still incomplete understanding of the diversity sources. The reactivity atmosphere has potential implications for both global warming and cooling, with human health also arising from isoprene-induced ozone formation urban areas. emissions terrestrial environments have been studied many years, our aquatic less complete. Several abundant phytoplankton taxa produced...

Bacteria that inhabit soils and the leaves of trees partially mitigate release abundant volatile organic compound, isoprene (2-methyl-1,3-butadiene). While initial steps metabolism were identified in Rhodococcus sp. AD45 two decades ago, metabolic pathway still remains largely undefined. Limited understanding functions isoG, isoJ aldH uncertainty route isoprene-derived carbon into central have hindered our metabolism. These previously uncharacterised iso genes are essential Variovorax WS11,...

Isoprene monooxygenase (IsoMO, encoded by isoABCDEF) initiates the oxidation of climate-active gas isoprene, with genes isoGHIJ and aldH nearly always found adjacent to isoABCDEF in extant metagenome-derived isoprene degraders. The roles are uncertain, although each is essential degradation. We report here characterization these proteins from two model degraders, Rhodococcus sp. strain AD45 Variovorax WS11. isoHIJ were expressed individually Escherichia coli as maltose binding protein...

Abstract Co‐oxidation of a range alkenes, dienes, and aromatic compounds by whole cells the isoprene‐degrading bacterium Rhodococcus sp. AD45 expressing isoprene monooxygenase was investigated, revealing relatively broad substrate specificity for this soluble diiron centre monooxygenase. A 1‐alkynes (C 2 –C 8 ) were tested as potential inhibitors. Acetylene, potent inhibitor related enzyme methane monooxygenase, had little inhibitory effect, whereas 1‐octyne indicating that could potentially...

Abstract Antimicrobial resistance (AMR) is widespread in terrestrial ecosystems. However, the natural processes shaping spatial and temporal dissemination of AMR soils are not well understood. We aimed to determine whether, how, why varies recently deglaciated pioneer developing Arctic soils. showed that antibiotic-resistant genes (ARGs), mobile genetic elements (MGEs), bacteria (ARB) abundant, exhibit a non-uniform distribution, generally increase with soil age. Our analyses suggest strong...

ABSTRACT Carbon monoxide (CO) degrading microorganisms are present in volcanic deposits throughout succession, with vegetation and soil influencing the communities present. The carboxydovores a subset of CO degraders that use only as an energy source, raising question how physiological metabolic features can make these bacteria more competitive harsh ecosystems. An enrichment strategy was modified, which enabled isolation two carboxydovore representatives from genera were abundant native...