A5032609853- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Odor and Emission Control Technologies
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Atmospheric chemistry and aerosols
- Wastewater Treatment and Nitrogen Removal
- Pesticide and Herbicide Environmental Studies
- Coastal wetland ecosystem dynamics
- Oil Palm Production and Sustainability
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Soil and Water Nutrient Dynamics
- Quantum Dots Synthesis And Properties
- Polysaccharides Composition and Applications
- Analytical chemistry methods development
- Proteins in Food Systems
- Water Treatment and Disinfection
- Mercury impact and mitigation studies
- Microbial Fuel Cells and Bioremediation
- Atmospheric and Environmental Gas Dynamics
- Plant biochemistry and biosynthesis
University of East Anglia
2017-2024
Ocean University of China
2023-2024
Norwich Research Park
2017-2023
Universitat de Barcelona
2010-2015
The volatile compound dimethylsulphide (DMS) is important in climate regulation, the sulphur cycle and signalling to higher organisms. Microbial catabolism of marine osmolyte dimethylsulphoniopropionate (DMSP) thought be major biological process generating DMS. Here we report discovery characterization first gene for DMSP-independent DMS production any bacterium. This gene, mddA, encodes a methyltransferase that methylates methanethiol generates MddA functions many taxonomically diverse...
Abstract Oxidation of methanethiol (MT) is a significant step in the sulfur cycle. MT an intermediate metabolism globally organosulfur compounds including dimethylsulfoniopropionate (DMSP) and dimethylsulfide (DMS), which have key roles marine carbon cycling. In aerobic bacteria, degraded by oxidase (MTO). The enzymatic genetic basis oxidation remained poorly characterized. Here, we identify for first time MTO enzyme its encoding gene (mtoX) DMS-degrading bacterium Hyphomicrobium sp. VS. We...
Abstract Dimethylsulfide (DMS) is an environmentally important trace gas with roles in sulfur cycling, signalling to higher organisms and atmospheric chemistry. DMS believed be predominantly produced marine environments via microbial degradation of the osmolyte dimethylsulfoniopropionate (DMSP). However, significant amounts are also generated from terrestrial environments, for example, peat bogs can emit ~6 μmol m−2 per day, likely methylation methanethiol (MeSH). A methyltransferase enzyme...
Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by Antarctic strain Pseudomonas fragi GC01. Here studied role volatile sulfur compounds (VSCs) in CdS QDs P. The nanoparticles was evaluated presence sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sources. Intracellular occurred with all sources tested. However, extracellular observed only cultures amended (Cys) (Met). Extracellular were characterized...
Dimethylsulfoxonium propionate (DMSOP) is a recently identified and abundant marine organosulfur compound with roles in oxidative stress protection, global carbon sulfur cycling and, as shown here, potentially osmotolerance. Microbial DMSOP cleavage yields dimethyl sulfoxide, ubiquitous metabolite, acrylate, but the enzymes responsible, their environmental importance, were unknown. Here we report mechanisms diverse heterotrophic bacteria, fungi phototrophic algae not previously known to have...
During the taxonomic investigation of cold-adapted bacteria from samples collected in Antarctic area South Shetland Islands, one Gram-reaction-negative, psychrotolerant, aerobic bacterium, designated strain M1 T , was isolated marine sediment on Deception Island. The organism rod-shaped, catalase- and oxidase-positive motile by means a polar flagellum. This psychrotolerant grew at temperatures ranging −4 °C to 34 °C. Phylogenetic studies based 16S rRNA gene sequences confirmed that isolate...
Ubiquitous and diverse marine microorganisms utilise the abundant organosulfur molecule dimethylsulfoniopropionate (DMSP), main precursor of climate-active gas dimethylsulfide (DMS), as a source carbon, sulfur and/or signalling molecules. However, it is currently difficult to discern which microbes actively catabolise DMSP in environment, why they do so pathways used.
Abstract Background Isoprene is the most abundantly produced biogenic volatile organic compound (BVOC) on Earth, with annual global emissions almost equal to those of methane. Despite its importance in atmospheric chemistry and climate, little known about biological degradation isoprene environment. The largest source terrestrial plants, oil palms, cultivation which expanding rapidly, are among highest isoprene-producing trees. Results DNA stable isotope probing (DNA-SIP) study microbial...
Approximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar that methane, and despite its significant effects on climate, very little is known about biological degradation in environment. Isolation characterisation degraders at molecular level has allowed development probes targeting isoA encoding α-subunit monooxygenase. This enzyme belongs soluble diiron centre monooxygenase family catalyses first step pathway. The use key metabolic genes a successful...
Methanethiol (MeSH) and dimethylsulfide (DMS) are volatile organic sulfur compounds (VOSCs) with important roles in cycling, signaling atmospheric chemistry. DMS can be produced from MeSH through a reaction mediated by the methyltransferase MddA. The mddA gene is present terrestrial marine metagenomes, being most abundant soil environments. substrate for MddA, MeSH, also oxidized bacteria oxidase (MTO) enzyme, encoded mtoX gene, found marine, freshwater metagenomes. Methanethiol-dependent...
The volatile secondary metabolite, isoprene, is released by trees to the atmosphere in enormous quantities, where it has important effects on air quality and climate. Oil palm trees, one of highest isoprene emitters, are increasingly dominating agroforestry over large areas Asia, with associated uncertainties their Microbes capable using as a source carbon for growth have been identified soils tree phyllosphere, most members Actinobacteria. Here, we used DNA stable isotope probing identify...
Abstract Volatile organic sulfur compounds (VOSCs) link the atmospheric, marine, and terrestrial cycles in marine marginal environments. Despite important role VOSCs play global biogeochemical cycling, less is known about how local geochemical conditions influence production consumption of VOSCs. We present a study dimethyl sulfide (DMS), methanethiol (MeSH), dimethylsulfoniopropionate (DMSP) sulfide‐rich (sulfidic) iron‐rich (ferruginous) salt marsh sediment from north Norfolk, UK. Initial...
Abstract Dimethylsulfoxonium propionate (DMSOP) is a recently identified and abundant marine organosulfur compound with purported roles in oxidative stress protection, global carbon sulfur cycling 1 . Diverse algae bacteria synthesise DMSOP from dimethylsulfoniopropionate (DMSP), which potentially limits the production of climate-active gases e.g., dimethylsulfide (DMS) generated microbial DMSP cleavage Here, was found at mM levels saltmarsh sediment, >10-fold higher than DMSP, orders...
In recent years, improvements in transmission electron microscopy (TEM) techniques and the use of tomography have provided a more accurate view complexity ultrastructure prokaryotic cells. Cryoimmobilization specimens by rapid cooling followed freeze substitution (FS) sectioning, fracture (FF) observation replica, or cryoelectron vitreous sections (CEMOVIS) now allow visualization biological samples close to their native state, enabling us refine our knowledge already known bacterial...
Billions of tons dimethylsulfoniopropionate (DMSP) are produced every year in marine and coastal ecosystems such as saltmarshes estuaries. DMSP has far-reaching roles global carbon sulfur cycling, also an osmotolerant signalling molecule. Furthermore, the microbial degradation contributes significantly to formation dimethylsulfide (DMS) methanethiol (MT), other abundant organosulfur compounds with ecological significance. Particularly, anaerobic sediments, DMS MT leads methane, a powerful...
Abstract Dimethylsulfoniopropionate (DMSP) is an abundant marine organosulfur compound with roles in stress protection, chemotaxis, nutrient and sulfur cycling climate regulation. Here we report the discovery of a bifunctional DMSP biosynthesis enzyme, DsyGD, transamination pathway rhizobacterium Gynuella sunshinyii some filamentous cyanobacteria not previously known to produce DMSP. DsyGD produces through its N-terminal DsyG methylthiohydroxybutyrate S -methyltransferase C-terminal DsyD...