Although Anoxybacillus and Geobacillus, two genera of thermophilic bacteria close to the genus Bacillus, have only been described recently, number species in these has increased rapidly. Four thermophilic, lipolytic strains (DR01, DR02, DR03 DR04) isolated from a hot spring Veracruz (Mexico), which could not be identified phenotypically, were subjected 16S rRNA gene sequence analysis. Three as belonging Anoxybacillus, but strain was Geobacillus pallidus. This result led us perform...

Five Aeromonas strains (848T T , 93M, 431E, 849T and 869N), which were isolated from bivalve molluscs recognized previously by numerical taxonomy as members of an unknown taxon, subjected to a polyphasic taxonomic study. DNA–DNA hybridization experiments showed that DNA strain 848T was <70 % similar (27–45 %) the type/reference current groups (HGs), but 93 93M. The G+C content five ranged 59·0 59·4 mol%. 16S rRNA gene sequence analysis confirmed belonged genus high similarity encheleia ....

A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and 868E T , isolated from bivalve molluscs, that could not be identified at species level in a previous numerical taxonomy study. The DNA G+C content these isolates 62.3 62.6 mol%, respectively. Sequence analysis 16S rRNA gene showed new strains were closely related members genus . Fluorescence amplified fragment length polymorphism fingerprinting revealed clustered together with similarity...

During the taxonomic investigation of cold-adapted bacteria from samples collected in Antarctic area South Shetland Islands, one Gram-reaction-negative, psychrotolerant, aerobic bacterium, designated strain M1 T , was isolated marine sediment on Deception Island. The organism rod-shaped, catalase- and oxidase-positive motile by means a polar flagellum. This psychrotolerant grew at temperatures ranging −4 °C to 34 °C. Phylogenetic studies based 16S rRNA gene sequences confirmed that isolate...

An analysis of the universal target (UT) sequence from cpn60 gene was performed in order to evaluate its usefulness phylogenetic and taxonomic studies as an identification marker for genus Aeromonas. Sequences 555 bp, corresponding UT region, were obtained a collection 35 strains representing all species subspecies From these sequences, range divergence 0-23.3% obtained, with mean 11.2+/-0.9%. Comparative analyses between gyrB, rpoD 16S rRNA sequences carried out same Aeromonas strain...

Extremophiles are microorganisms that possess application possibilities in several industrial fields, including agricultural, chemical, laundry, pharmaceutical, food, petroleum and bioremediation. This work reports the isolation of 19 thermophilic, alkalitolerant halotolerant bacterial strains from two thermal sites Veracruz, México: El Carrizal pool Los Baños hot spring. These belong to Geobacillus, Anoxybacillus Aeribacillus genera. The produce lipases, proteases, amylases under...

Journal Article Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from environmental clinical samples in Spain Get access D. Miñana‐Galbis, Miñana‐Galbis Departament Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Divisió IV, Universitat Barcelona, Search for other works by this author on: Oxford Academic Google Scholar M. Farfán, Farfán J.G. Lorén, Lorén M.C. Fusté Applied Microbiology, Volume 93, Issue 3, 1 September 2002, Pages 420–430,...

Two strains of psychrotolerant bacteria, designated M7T and M5, isolated from Antarctic coastal marine environments were studied to determine their taxonomic position. The organisms comprised Gram-negative, rod-shaped, facultatively anaerobic cells that motile by means single polar flagella. Neither the bacterial isolates had a requirement for Na+. These two grew at temperatures ranging −4 30 °C. Both capable producing H2S thiosulfate able use sodium nitrate trimethylamine N-oxide as...

ABSTRACT Comparative analysis of gene fragments six housekeeping loci, distributed around the two chromosomes Vibrio cholerae , has been carried out for a collection 29 V. O139 Bengal strains isolated from India during first epidemic period (1992 to 1993). A toxigenic O1 ElTor strain seventh pandemic and an environmental non-O1/non-O139 were also included in this study. All loci studied polymorphic, with small number polymorphic sites sequenced fragments. The genetic diversity determined our...

Aeromonas are autochthonous inhabitants of aquatic environments, including chlorinated and polluted waters, although they can also be isolated from a wide variety environmental clinical sources. They cause infections in vertebrates invertebrates considered to an emerging pathogen humans, producing intestinal extra-intestinal diseases. Most the isolates correspond A. hydrophila, caviae, veronii bv. Sobria, which described as causative agents wound infections, septicaemia, meningitis...

ABSTRACT Plesiomonas shigelloides , the only species of genus, is an emergent pathogenic bacterium associated with human diarrheal and extraintestinal disease. We present whole-genome sequence analysis representative strain for O1 serotype (strain 302-73), providing a tool studying bacterial outbreaks, virulence factors, accurate diagnostic methods.

During the taxonomic investigation of exopolymer-producing halophilic bacteria, a rod-shaped, motile, Gram-stain-negative, bacterium, designated strain N4T, was isolated from saline soil located in northern Morocco. Optimal growth isolate at 30-37 ºC and pH 7.0-8.0, presence 5-7 % (w/v) NaCl. Useful characteristics for phenotypic differentiation N4T other Marinobacter species included α-chymotrypsin α-glucosidase activities carbohydrate assimilation profile. The major fatty acids detected...

Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-negative, aerobic, catalase- and oxidase-positive gammaproteobacterial strain, referred to as FIT28 T , was isolated. shows vigorous growth on nutrient rich media within the temperature range 4–35 °C, with optimal at 28 wide pH tolerance 5 11, salt up 6 % (w/v) NaCl. Colonies are white-yellow quickly become mucoid. The results analysis 16S rRNA gene sequence placed strain genus Pseudomonas...

The use of reference strains is a critical element for the quality control different assays, from development molecular methods to evaluation antimicrobial activities. Most used in these assays are not type and some them cited erroneously because subsequent reclassifications descriptions novel species. In this study, we propose that strain Aeromonas hydrophila CIP 57.50 be reclassified as salmonicida based on phenotypic characterization sequence analyses cpn60 , dnaJ gyrB rpoD genes.

The bacterial flagellum is the most important organelle of motility in bacteria and plays a key role many lifestyles, including virulence. also provides paradigm how hierarchical gene regulation, intricate protein-protein interactions controlled protein secretion can result assembly complex multi-protein structure tightly orchestrated time space. As if to stress its importance, plants animals produce receptors specifically dedicated recognition flagella. Aside from motility, moonlights as an...

Summary Genetic diversity, genetic relationship, identification and population structure of 120 Aeromonas strains (including Aer. hydrophila , bestiarum salmonicida popoffii ) isolated from various sources were studied by analysis 15 loci multilocus enzyme electrophoresis (MLEE). All polymorphic, with an average 9.4 alleles per locus a mean diversity ( H 0.64. Cluster defined at ≤ 0.7 differentiated most the taxa analysed except strains, which showed close relationship. Allelic frequencies...

Helicobacter pylori is a common pathogen associated with several severe digestive diseases. Although multiple virulence factors have been described, it still unclear the role of on H. pathogenesis and disease progression. Whole genome sequencing could help to find genetic markers strains. In this work, we analyzed three complete genomes from isolates obtained at same point in time stomach patient adenocarcinoma, using available bioinformatics tools. The analysis strains B508A-S1, B508A-T2A...

Helicobacter pylori infects human stomachs of over half the world's population, evades immune response and establishes a chronic infection. Although most people remains asymptomatic, duodenal gastric ulcers, MALT lymphoma progression to cancer could be developed. Several virulence factors such as flagella, lipopolysaccharide, adhesins especially vacuolating cytotoxin VacA oncoprotein CagA have been described for H. pylori. Despite extensive published data on pylori, more research is needed...