A5062856803- Metalloenzymes and iron-sulfur proteins
- Electrocatalysts for Energy Conversion
- CO2 Reduction Techniques and Catalysts
- Advanced battery technologies research
- Microbial metabolism and enzyme function
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Hydrogen Storage and Materials
- Ammonia Synthesis and Nitrogen Reduction
- Catalytic Processes in Materials Science
Massachusetts Institute of Technology
2023
Stanford University
2018-2021
Abstract Molecular hydrogen is a major high‐energy carrier for future energy technologies, if produced from renewable electrical energy. Hydrogenase enzymes offer pathway bioelectrochemically producing that advantageous over traditional platforms production because of low overpotentials and ambient operating temperature pressure. However, electron delivery the electrode surface to enzyme's active site often rate‐limiting. Here, it shown three different hydrogenases Clostridium pasteurianum...
Hydrogenotrophic methanogens oxidize molecular hydrogen to reduce carbon dioxide methane. In without cytochromes, the initial endergonic reduction of CO2 formylmethanofuran with H2-derived electrons is coupled exergonic a heterodisulfide coenzymes B and M by flavin-based electron bifurcation (FBEB). Methanococcus maripaludis, FBEB performed reductase (Hdr) enzyme complex that involves hydrogenase (Vhu), although formate dehydrogenase (Fdh) has been proposed as an alternative Vhu. We have...
While enzymes catalyze reactions with high selectivity and specificity at ambient temperature pressure, electroactive retain such remarkable catalytic properties for catalyzing redox on the basis of direct or mediated electron transfer an electrode. They offer possibility alternative, environmentally friendly production a variety industrially relevant chemicals, as hydrogen gas, ammonia, methanol. This Perspective summarizes recent progress in electrochemistry involving hydrogenases,...
High-temperature tolerant enzymes offer multiple advantages over from mesophilic organisms for the industrial production of sustainable chemicals due to high specific activities and stabilities towards fluctuations in pH, heat, organic solvents. The molecular hydrogen (H2) is particular interest because uses energy applications, ability hydrogenase reduce protons H2 at a cathode. We examined activity Hydrogen-Dependent CO2 Reductase (HDCR) thermophilic bacterium Thermoanaerobacter kivui when...
Methanogenic archaea can be integrated into a sustainable, carbon-neutral cycle for producing organic chemicals from C1 compounds if the rate, yield, and titer of product synthesis improved using metabolic engineering. However, engineering techniques are limited in methanogens by insufficient methods controlling cellular protein levels. We conducted systematic approach to tune levels Methanosarcina acetivorans C2A, model methanogen, regulating transcription translation initiation. Rationally...