Systemic acquired resistance (SAR) is a global response in plants induced at the site of infection that leads to long-lasting and broad-spectrum disease distal, uninfected tissues. Despite importance this priming mechanism, identity complexity defense signals are required initiate SAR signaling not well understood. In paper, we describe metabolite, N-hydroxy-pipecolic acid (N-OH-Pip) provide evidence mobile molecule plays role initiating signal transduction Arabidopsis thaliana We...

Systemic acquired resistance (SAR) is a mechanism that plants utilize to connect local pathogen infection global defense responses. N-hydroxy-pipecolic acid (NHP) and glycosylated derivative are produced during SAR, yet their individual roles in this process currently unclear. Here, we report Arabidopsis thaliana UGT76B1 generated NHP (NHP-Glc) vitro when transiently expressed alongside biosynthetic genes two Solanaceous plants. During infection, ugt76b1 mutants did not accumulate NHP-Glc...

Systemic acquired resistance (SAR) is a powerful immune response that triggers broad-spectrum disease throughout plant. In the model plant Arabidopsis thaliana, long-distance signaling and SAR activation in uninfected tissues occur without circulating cells instead rely on metabolite N-hydroxy-pipecolic acid (NHP). Engineering crop plants would enable external control of plant's ability to mount global defense upon sudden changes environment. Such metabolite-engineering approach require...

Global food production is a major contributor to greenhouse gas emissions, water consumption, and land use. As an alternative conventional agriculture, the of waste-derived microbial protein (MP) holds promise for reducing environmental impacts. MP can be mass-produced in volumetrically scalable fermentation processes on short time scales, enabling facile scale-up with lower use, impacts than animal and, some cases, plant protein. also produced from waste feedstocks, diverting landfills or...

Rhodopseudomonas palustris is a versatile microbe that encounters an innate redox imbalance while growing photoheterotrophically with reduced substrates. The resulting excess in reducing equivalents, together ATP from photosynthesis, could be utilized to drive wide range of bioconversions. objective this study was genetically modify R. provide pathway reduce n-butyrate into n-butanol for maintaining balance. Here, we constructed and expressed plasmid-based production Clostridium...

The unique physiological properties of fungi are useful for a myriad applications, which could greatly benefit from increased control native pathways and introduction recombinant genes. However, fungal genetic engineering is still limited in scope accessibility, largely due to lack standardization. To help standardize the filamentous fungi, we created BioBricks commonly used antibiotic resistance genes, neomycin phosphotransferase (nptII) hygromycin (hph), confer G418 (Geneticin) B,...

Abstract Systemic acquired resistance (SAR) is a global response in plants induced at the site of infection that leads to long-lasting and broad-spectrum disease distal, uninfected tissues. Despite importance this priming mechanism, identity mobile defense signal moves systemically throughout initiate SAR has remained elusive. In paper, we describe new metabolite, N -hydroxy-pipecolic acid (N-OH-Pip), provide evidence molecule plays central role initiating transduction Arabidopsis thaliana ....

Weatherization of residential homes is a widespread procedure to retrofit older improve the energy efficiency by reducing building leakage. Several studies have evaluated effect weatherization on indoor pollutants, such as formaldehyde, radon, and particulates, but few microbial exposure. Here, we monitored pollutants bacterial communities during reductions in leakage for weatherized single-family New York State compared data non-weatherized homes. Nine eleven Tompkins County, were sampled...

Abstract Systemic acquired resistance (SAR) is a mechanism that plants utilize to connect local pathogen infection global defense responses. N -hydroxy-pipecolic acid (NHP) and glycosylated derivative are produced during SAR, yet their individual roles in the response have not been elucidated. Here we report Arabidopsis thaliana UGT76B1 can generate NHP (NHP-Glc) vitro when transiently expressed alongside biosynthetic genes two Solanaceous plants. During infection, ugt76b1 mutants do...

Methanogenic archaea can be integrated into a sustainable, carbon-neutral cycle for producing organic chemicals from C1 compounds if the rate, yield, and titer of product synthesis improved using metabolic engineering. However, engineering techniques are limited in methanogens by insufficient methods controlling cellular protein levels. We conducted systematic approach to tune levels Methanosarcina acetivorans C2A, model methanogen, regulating transcription translation initiation. Rationally...

SUMMARY Signal propagation and the coordination of whole-organism responses in plants rely heavily on small molecules. Systemic acquired resistance (SAR) is one such process which long-distance signaling activates immune uninfected tissue as a way to limit spread primary, localized infection. Recently, N -hydroxy pipecolic acid (NHP) was discovered shown coordinate SAR Arabidopsis . Here, we provide metabolic biochemical evidence that NHP conserved across plant kingdom demonstrate role for...

Abstract Weatherization of residential homes is a popular retrofit procedure to improve the energy efficiency older by reducing building leakage. It vital tool in fight against climate change. Several studies have evaluated effect weatherization on indoor pollutants such as formaldehyde and radon, but few particulates microbial exposure. In this study, we compared change leakage bacterial communities weatherized non-weatherized single-family New York State. Nine eleven Tompkins County, were...