A5058345099- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Spaceflight effects on biology
- biodegradable polymer synthesis and properties
- Environmental Science and Technology
- Biomedical and Engineering Education
- Biochemical and biochemical processes
- Plant biochemistry and biosynthesis
- 3D Printing in Biomedical Research
- Microbial metabolism and enzyme function
- Amino Acid Enzymes and Metabolism
- Biomedical and Chemical Research
- Biochemical Acid Research Studies
- Fungal and yeast genetics research
- Metabolism and Genetic Disorders
- Algal biology and biofuel production
- Gene Regulatory Network Analysis
- Polyamine Metabolism and Applications
- Plant Toxicity and Pharmacological Properties
- Botanical Research and Chemistry
- Pharmacological Effects of Natural Compounds
- Microplastics and Plastic Pollution
- Photoreceptor and optogenetics research
- Microbial Natural Products and Biosynthesis
Stanford University
2020-2024
Space Micro (United States)
2021
Universities Space Research Association
2017-2020
Ames Research Center
2017-2020
Australian Water Quality Centre
2017
The University of Queensland
2012-2017
TU Dortmund University
2012-2014
The aromatic nature of shikimate pathway intermediates gives rise to a wealth potential bio-replacements for commonly fossil-fuel derived aromatics, as well naturally produced secondary metabolites. Through metabolic engineering, the abundance certain may be increased, while draining flux from other branches off pathway. Often targets genetic engineering lie beyond pathway, altering deep in central metabolism. This has been extensively used develop microbial production systems variety...
A crewed mission to and from Mars may include an exciting array of enabling biotechnologies that leverage inherent mass, power, volume advantages over traditional abiotic approaches. In this perspective, we articulate the scientific engineering goals constraints, along with example systems, guide design a surface biomanufactory. Extending past arguments for exploiting stand-alone elements biology, argue integrated biomanufacturing plant replete modules microbial in situ resource utilization,...
There is currently a strong interest to derive the biological precursor cis,cis-muconic acid from shikimate pathway-branches develop replacement for adipic acid. Pioneered by Frost laboratory this concept has regained interest: Recent approaches (Boles, Alper, Yan) however suffer low product titres. Here an in silico comparison of all strain construction strategies was conducted highlight stoichiometric optimizations. Using elementary mode analysis new knock-out were determined Saccharomyces...
Biological production of the aromatic compound para-aminobenzoic acid (pABA) is great interest to chemical industry. Besides its application in pharmacy and as crosslinking agent for resins dyes pABA a potential precursor high-volume feedstocks terephthalic para-phenylenediamine. The yeast Saccharomyces cerevisiae synthesises shikimate pathway: Outgoing from central pathway intermediate chorismate, formed two enzyme-catalysed steps, encoded by genes ABZ1 ABZ2. In this study S. metabolism was...
High-strength polymers, such as aramid fibres, are important materials in space technology. To obtain these remote locations, Mars, biological production is of interest. The aromatic polymer precursor para-aminobenzoic acid (pABA) can be derived from the shikimate pathway through metabolic engineering Bacillus subtilis, an organism suited for synthetic biology. Our strategy included repair defective indole-3-glycerol phosphate synthase (trpC), knockout one chorismate mutase isozyme (aroH)...
Various microbial systems have been explored for their applicability to in-situ resource utilisation (ISRU) on Mars and suitability leverage Martian resources convert them into useful chemical products. Considering only fully bio-based solutions, two approaches can be distinguished, which comes down the form of carbon that is being utilized: (a) deployment specialised species directly inorganic (atmospheric CO 2 ) a target compound or (b) two-step process relies independent fixation...
Abstract Space bioprocess engineering (SBE) is an emerging multi-disciplinary field to design, realize, and manage biologically-driven technologies specifically with the goal of supporting life on long term space missions. SBE considers synthetic biology under extreme constraints conditions space. A coherent strategy for development this lacking. In Perspective, we describe need expanded mandate explore biotechnological needs future We then identify several key parameters—metrics,...
Adipic acid, a nylon-6,6 precursor, has recently gained popularity in synthetic biology. Here, 16 different production routes to adipic acid were evaluated using novel tool for network-embedded thermodynamic analysis of elementary flux modes. The distinguishes between thermodynamically feasible and infeasible modes under determined metabolite concentrations, allowing the feasibility theoretical yields be assessed. Further, patterns that always caused distributions identified, which will aid...
In Space, cosmic radiation is a strong, ubiquitous form of energy with constant flux, and the ability to harness it could greatly enhance energy-autonomy expeditions across solar system. At same time, greatest permanent health risk for humans venturing into deep space. To protect astronauts beyond Earth's magnetosphere, advanced shielding against ionizing as well non-ionizing highly sought after. search innovative solutions these challenges, biotechnology appeals suitability in situ resource...
Metabolic engineering of microbial strains to produce aromatic compounds deriving from the shikimate pathway is great interest chemical industry as a more sustainable alternative for feedstock production. Chorismate significant intermediate in pathway. In this study, formation phenylalanine and phenylpyruvate by-products engineered downstream chorismate node increased production was explored yeast fermentations. Tracer experiments showed that these are synthesized de novo during...
Abstract The greatest hazard for humans on deep-space exploration missions is radiation. To protect astronauts venturing out beyond Earth’s protective magnetosphere, advanced passive radiation protection highly sought after. In search of innovative radiation-shields, biotechnology appeals with suitability in-situ resource utilization (ISRU), self-regeneration, and adaptability. Certain fungi thrive in high-radiation environments Earth, such as the contamination radius Chernobyl Nuclear Power...
A crewed mission to and from Mars may include an exciting array of enabling biotechnologies that leverage inherent mass, power, volume advantages over traditional abiotic approaches. In this perspective, we articulate the scientific engineering goals constraints, along with example systems, guide design a surface biomanufactory. Extending past arguments for exploiting stand-alone elements biology, argue integrated biomanufacturing plant replete modules microbial \textit{in situ} resource...
The potent greenhouse gas methane presents a widely accessible resource, being the primary component in natural as well bio-gas from anaerobic digesters. Given its relatively low heating-value and several issues concerning storage transportation, upgrading to liquid fuels is of particular interest. Microbial conversion/utilization gaining increasing interest due high conversion efficiency. In this study we computationally compare aerobic microbial pathways for CH4 oxidation discuss...
The replacement of petrochemical aromatics with bio-based molecules is a key area current biotechnology research. To date, small number have been produced by recombinant bacteria in laboratory scale while industrial production still requires further strain development. While each study includes some distinct analytical methodology to quantify certain aromatics, method that can reliably great aromatic products and relevant pathway intermediates needed accelerate In this study, we developed...