A5074576220- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Extracellular vesicles in disease
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Histone Deacetylase Inhibitors Research
- Renal and related cancers
- Fish Ecology and Management Studies
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- Chronic Myeloid Leukemia Treatments
- Urologic and reproductive health conditions
- Hematopoietic Stem Cell Transplantation
- Underwater Vehicles and Communication Systems
- Chronic Lymphocytic Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Reproductive biology and impacts on aquatic species
- IoT Networks and Protocols
- Telomeres, Telomerase, and Senescence
- Indoor and Outdoor Localization Technologies
- Advanced biosensing and bioanalysis techniques
Icahn School of Medicine at Mount Sinai
2016-2024
Huazhong Agricultural University
2022-2023
Northeastern University
2020
National University of Singapore
2011-2016
Cancer driver mutations often show distinct temporal acquisition patterns, but the biological basis for this, if any, remains unknown. RAS occur invariably late in course of acute myeloid leukaemia, upon progression or relapsed/refractory disease1–6. Here, by using human leukaemogenesis models, we first that are obligatory events need to succeed earlier cooperating mutations. We provide mechanistic explanation this a requirement mutant specifically transform committed progenitors...
Stem cell cultures can be derived directly from early developing embryos and indirectly differentiated cells by forced expression of pluripotency transcription factors.Pluripotency genes are routinely used to characterize mammalian stem at the molecular level.However, such have remained unknown in lower vertebrates.In this regard, laboratory fish medaka is uniquely suited because it has embryonic (ES) genome sequence data.We identified seven homology search vivo vitro.By RT-PCR analysis,...
Primordial germ cell (PGC) specification occurs early in development. PGC specifiers have been identified Drosophila, mouse, and human but remained elusive most animals. Here we identify the RNA-binding protein Dnd as a critical specifier medaka fish (Oryzias latipes). depletion specifically abolished PGCs, its overexpression boosted PGCs. We established single-cell culture procedure enabling lineage tracing vitro. show that individual blastomeres from cleavage embryos at 32- 64-cell stages...
Sertoli cells contribute to the formation of blood-testis barrier (BTB), which is necessary for normal spermatogenesis. Recently, microRNAs (miRNAs) have emerged as posttranscriptional regulatory elements in BTB function during Our previous study has shown that miR-181c or miR-181d (miR-181c/d) highly expressed testes from boars at 60 days old compared with 180 old. Herein, we found overexpression miR-181c/d via mimics murine (SCs) through injecting miR-181c/d-overexpressing lentivirus...
Embryonic stem (ES) cells have immortality for self-renewal and pluripotency.Differentiated human undergo replicative senescence.In human, the telomerase reverse transcriptase (Tert), namely catalytic subunit of telomerase, exhibits differential expression to regulate activity governing cellular or senescence, tert is a routine marker pluripotent ES cells.Here we identified medaka gene determined its in vivo vitro.We found that locus produces five variants called terta terte encoding...
Gene targeting (GT) provides a powerful tool for the generation of precise genetic alterations in embryonic stem (ES) cells to elucidate gene function and create animal models human diseases. This technology has, however, been limited mouse rat. We have previously established ES cell lines procedures transfer selection homologous recombination (HR) events fish medaka (Oryzias latipes).Here we report HR-mediated GT this organism. designed vector disrupt tumor suppressor p53 (also known as...
BCL2-associated athanogene 6 (BAG6) plays critical roles in spermatogenesis by maintaining testicular cell survival. Our previous data showed porcine BAG6 exon24-skipped transcript is highly expressed immature testes compared with mature testes. The objective of this study to reveal the functional significance exon24 mammalian spermatogenesis.CRISPR/Cas9 system was used generate Bag6 knockout mice. Testes and cauda epididymal sperm were collected from TMT proteomics analysis discover protein...
Sperm associated antigen 6 (SPAG6) acts as a scaffolding protein in the center of flagellar axoneme and has an impact on maturation motility mammalian sperm flagella maintenance structure. In our previous research, SPAG6 c.900 T>C exon 7 skipped transcript was identified by analyzing RNA-seq data testicular tissues from 60 day (sexually immature) 180 mature) Large White boars. Herein, we found porcine to be with semen quality traits Duroc, Landrace pigs. C can generate new splice acceptor...
Abstract Human cancers arise through an evolutionary process whereby cells acquire somatic mutations that drive them to outgrow normal and create successive clonal populations. “Bottom-up” human cancer evolution models could help illuminate this process, but their creation has faced significant challenges. Here we combined induced pluripotent stem cell (iPSC) CRISPR/Cas9 technologies develop a model of the acute myeloid leukemia (AML). Through sequential introduction 3 disease-causing (ASXL1...
<div>Abstract<p>The reprogramming of human acute myeloid leukemia (AML) cells into induced pluripotent stem cell (iPSC) lines could provide new faithful genetic models AML, but is currently hindered by low success rates and uncertainty about whether iPSC-derived resemble their primary counterparts. Here we developed a method tailored to cancer cells, with which generated iPSCs from 15 patients representing all major groups AML. These AML-iPSCs retain fidelity produce...
<p>Supplemental Figure 1. Generation of a panel iPSCs from patients with AML. Supplemental 2. Reprogramming aids reconstruction the evolutionary history and clonal composition 3. Transplantation AML-iPSCs into immunodeficient mice. 4. Developmental block in subset AML-iPSC lines. 5. primary AML cells patient-matched AMLiPSC 6. Single-cell RNA-sequencing analyses matched iPSC-derived leukemia patient AML-47. 7. Cell cycle pseudotime analyses. 8. Comparison scRNA-Seq data integration...
<div>Abstract<p>The reprogramming of human acute myeloid leukemia (AML) cells into induced pluripotent stem cell (iPSC) lines could provide new faithful genetic models AML, but is currently hindered by low success rates and uncertainty about whether iPSC-derived resemble their primary counterparts. Here we developed a method tailored to cancer cells, with which generated iPSCs from 15 patients representing all major groups AML. These AML-iPSCs retain fidelity produce...
<p>Supplemental Figure 1. Generation of a panel iPSCs from patients with AML. Supplemental 2. Reprogramming aids reconstruction the evolutionary history and clonal composition 3. Transplantation AML-iPSCs into immunodeficient mice. 4. Developmental block in subset AML-iPSC lines. 5. primary AML cells patient-matched AMLiPSC 6. Single-cell RNA-sequencing analyses matched iPSC-derived leukemia patient AML-47. 7. Cell cycle pseudotime analyses. 8. Comparison scRNA-Seq data integration...
<div>Abstract<p>The reprogramming of human acute myeloid leukemia (AML) cells into induced pluripotent stem cell (iPSC) lines could provide new faithful genetic models AML, but is currently hindered by low success rates and uncertainty about whether iPSC-derived resemble their primary counterparts. Here we developed a method tailored to cancer cells, with which generated iPSCs from 15 patients representing all major groups AML. These AML-iPSCs retain fidelity produce...
<p>Supplemental Figure 1. Generation of a panel iPSCs from patients with AML. Supplemental 2. Reprogramming aids reconstruction the evolutionary history and clonal composition 3. Transplantation AML-iPSCs into immunodeficient mice. 4. Developmental block in subset AML-iPSC lines. 5. primary AML cells patient-matched AMLiPSC 6. Single-cell RNA-sequencing analyses matched iPSC-derived leukemia patient AML-47. 7. Cell cycle pseudotime analyses. 8. Comparison scRNA-Seq data integration...