A5017643098- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- RNA Research and Splicing
- Chronic Myeloid Leukemia Treatments
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- RNA regulation and disease
- Nanoplatforms for cancer theranostics
- Constructed Wetlands for Wastewater Treatment
- Virus-based gene therapy research
- RNA modifications and cancer
- Biological Control of Invasive Species
- Viral Infectious Diseases and Gene Expression in Insects
- Aquaculture disease management and microbiota
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Ubiquitin and proteasome pathways
University of Rochester
2019-2025
Memorial Sloan Kettering Cancer Center
2015-2025
Vanderbilt University Medical Center
2025
University of Rochester Medical Center
2013-2024
Indiana University – Purdue University Indianapolis
2024
Indiana University School of Medicine
2024
Columbia University
2016-2022
National Taiwan University
2008
Chung Hwa University of Medical Technology
2008
CRISPR/Cas9 technology enables targeted gene editing; yet, the efficiency and specificity remain unsatisfactory, particularly for nonvirally delivered, plasmid-based system. To tackle this, a self-assembled micelle is developed evaluated human papillomavirus (HPV) E7 oncogene disruption. The optimized effective delivery of Cas9 plasmid with transient transgene expression profile, benefiting recognition. Furthermore, feasibility using explored another nucleic acid-guided nuclease system,
A scaffold-mediated system was developed to enhance the local delivery of Cas9 complexes for targeting leukemia stem cells in AML.
Abstract Periodontitis is a common type of inflammatory bone loss and risk factor for systemic diseases. The pathogenesis periodontitis involves dysregulation, which represents target new therapeutic strategies to treat periodontitis. After establishing the correlation cell-free DNA (cfDNA) level with in patient samples, we test hypothesis that cfDNA-scavenging approach will benefit treatment. We create nanoparticulate cfDNA scavenger specific by coating selenium-doped hydroxyapatite...
Recent advances in CRISPR present attractive genome-editing toolsets for therapeutic strategies at the genetic level. Here, a liposome-coated mesoporous silica nanoparticle (lipoMSN) is reported as an effective delivery system multiplex gene-editing liver. The MSN provides efficient loading of Cas9 plasmid well protein/guide RNA ribonucleoprotein complex (RNP), while liposome-coating offers improved serum stability and enhanced cell uptake. Hypothesizing that loss-of-function mutation...
Somatic mutations that increase hematopoietic stem cell (HSC) fitness drive their expansion in clonal hematopoiesis (CH) and predispose to blood cancers. Although CH frequently occurs with aging, it rarely progresses overt malignancy. Population variation the growth rate potential of mutant clones suggests presence genetic factors protecting against CH, but these remain largely undefined. Here, we identify a non-coding regulatory variant, rs17834140-T, significantly protects myeloid...
Abstract Myelodysplastic syndromes (MDS) are driven by complex genetic and epigenetic alterations. The MSI2 RNA-binding protein has been demonstrated to have a role in acute myeloid leukaemia stem cell function, but its MDS is unknown. Here, we demonstrate that elevated expression correlates with poor survival MDS. Conditional deletion of Msi2 mouse model results rapid loss haematopoietic progenitor cells (HSPCs) reverses the clinical features Inversely, inducible overexpression drives...
We propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells (APCs) for adoptive therapy (ACT). Microfiber scaffolds coated with dendritic (DC) membrane replicate physicochemical properties of specific activation such as rapid recognition by cells, long duration tethering, and DC-specific co-stimulatory cues. The DC membrane-coated scaffold is first surface-immobilized stimulatory ligands, anti-CD3 (αCD3) anti-CD28 (αCD28) antibodies, followed adsorption...
Abstract Radiotherapy is essential for treating unresectable or metastatic breast cancer, but radiotherapy‐induced tumor cell death generates small extracellular vesicles (sEVs) that promote regrowth and metastasis following treatment. Here cationic nanosheets with a high sEVs‐binding capacity suppress sEVs‐induced radiotherapy developed. Molybdenum disulfide (MoS 2 ) monolayers are prepared by using lithium ions as intercalation agents, functionalized polyamidoamine (PAMAM) dendrimers. The...
This study explored resistance functions and their interactions in de novo AML treated with the "7 + 3" induction regimen.We analyzed RNA-sequencing profiles of whole bone marrow samples from 52 patients who completed regimen stratified into CR (n = 35) non-CR 17) groups.A systematic gene set analysis revealed significant associations between chemoresistance mTOR (P < .001), myc mitochondrial oxidative phosphorylation stemness .002). These were independent regard to contents activity scores....
Water hyacinth (Eichhornia crassipes) is a rapid-growing freshwater vascular plant that has been used to remove heavy metals in contaminated water. But the transportation and distribution of absorbed metal are not clear. In this study, water was exposed cadmium (Cd, 10 μM, pulse) then transferred Cd-free solution (chase). The Cd content tissues measured, Cd-binding complexes were isolated identified. We found (1) two days, up 80% by plant, could be released back growth chase period; (2)...
Therapies for acute myeloid leukemia (AML) face formidable challenges due to relapse, often driven by stem cells (LSCs). Strategies targeting LSCs hold promise enhancing outcomes, yet paired comparisons of functionally defined at diagnosis and relapse remain underexplored. We present transcriptome analyses LSC populations revealing significant alterations in IL-1 signaling. Interleukin-1 receptor type I (IL1R1) interleukin-1 accessory protein (IL1RAP) were notably upregulated progenitor both...
SUMMARY Mesenchymal stem/stromal cells (MSCs) within the bone marrow microenvironment (BMME) support normal hematopoietic stem and progenitor (HSPCs). However, heterogeneity of human MSCs has limited understanding their contribution to clonal dynamics evolution myelodysplastic syndromes (MDS). We combined three MSC cell surface markers, CD271, VCAM-1 (Vascular Cell Adhesion Molecule-1) CD146, isolate distinct subsets from aspirates healthy controls (Control BM). Based on transcriptional...