A5035777674- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Zebrafish Biomedical Research Applications
- EEG and Brain-Computer Interfaces
- Herpesvirus Infections and Treatments
- Sleep and Wakefulness Research
- Parkinson's Disease Mechanisms and Treatments
- Endoplasmic Reticulum Stress and Disease
- Cytomegalovirus and herpesvirus research
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Medicinal Plants and Neuroprotection
- Cardiac electrophysiology and arrhythmias
- ATP Synthase and ATPases Research
- Functional Brain Connectivity Studies
- Toxoplasma gondii Research Studies
University of Birmingham
2016-2025
Obuda University
2025
University of Pecs
2018-2025
Shandong Academy of Sciences
2025
Qilu University of Technology
2025
Innova (Hungary)
2025
International Collaboration On Repair Discoveries
2019
McGill University
2010-2017
Université Laval
2002-2010
Hungarian Academy of Sciences
1993-2006
To understand the functional significance and mechanisms of action in CNS endogenous exogenous cannabinoids, it is crucial to identify neural elements that serve as structural substrate these actions. We used a recently developed antibody against CB1 cannabinoid receptor study this question hippocampal networks. Interneurons with features typical basket cells showed selective, intense staining for all subfields layers. Most them (85.6%) contained cholecystokinin (CCK), which corresponded...
Sharp wave bursts, induced by a cooperative discharge of CA3 pyramidal cells, are the most synchronous physiological pattern in hippocampus. In conjunction with sharp CA1 cells display high-frequency (200 Hz) network oscillation (ripple). present study extracellular field and unit activity was recorded simultaneously from 16 closely spaces sites awake rat intracellular during studied under anesthesia. Current source density analysis revealed circumscribed sinks sources vicinity layer. Single...
Dentate granule cells communicate with their postsynaptic targets by three distinct terminal types. These include the large mossy terminals, filopodial extensions of and smaller en passant synaptic varicosities. We examined fibers combining in vivo intracellular labeling cells, immunocytochemistry, electron microscopy. Single formed large, complex “mossy” synapses on 11–15 CA3 pyramidal 7–12 hilar cells. In contrast, GABAergic interneurons, identified immunostaining for substance P-receptor,...
Fast spiking interneurons in the CA1 area of dorsal hippocampus were recorded from and filled with biocytin anesthetized rats. The full extent their dendrites axonal arborizations as well calcium binding protein content examined. Based on spatial axon collaterals, local circuit cells (basket O-LM neurons) long-range (bistratified, trilaminar, backprojection could be distinguished. Basket immunoreactive for parvalbumin collaterals confined to pyramidal layer. A single basket cell contacted...
Abstract The cellular‐synaptic generation of rhythmic slow activity (RSA or theta) in the hippocampus has been investigated by intracellular recording from principal cells and basket anesthetized rats. In addition, voltage‐, coherence‐, phase versus depth profiles were examined simultaneously field at 16 sites intact rat, during urethane anesthesia, after bilateral entorhinal cortex lesion. extracellular experiments large peak theta hippocampal fissure was attenuated anesthesia abolished...
Abstract Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe formation spatio-temporal dynamics pair distinct transcription compartments, which encompasses earliest gene expression in zebrafish. 4D imaging pri- miR430 zinc-finger-gene activities by novel, native approach reveals...
Abstract Immunocytochemical visualization of the neuron‐specific K + /Cl – cotransporter, KCC2, at cellular and subcellular level revealed an area‐ layer‐specific diffuse labelling, a discrete staining outlining somata dendrites some interneurons in all areas rat hippocampus. KCC2 was highly expressed parvalbumin‐containing interneurons, as well subsets calbindin, calretinin metabotropic glutamate receptor 1a‐immunoreactive interneurons. During first 2 postnatal weeks, increase observed...
The organization of the hippocampus is generally thought as a series cell groups that form unidirectionally excited chain, regulated by localized inhibitory circuits. With use in vivo intracellular labeling, histochemical, and extracellular tracing methods, longitudinally widespread, feedback rat brain from CA1 area to CA3 hilar regions was observed. This long-range, cross-regional inhibition may allow precise synchronization population activity timing occurrence action potentials principal...
Abstract Interneurons in the dentate area were characterized physiologically and filled with biocytin urethane‐anaesthetized rats. On basis of axonal targets following groups could be distinguished. (i) Large multipolar interneurons spiny dendrites deep hilar region densely innervated outer molecular layer contacted both granule cells parvalbumin‐positive neurons (hilar interneuron perforant pathway‐associated axon terminals; HIPP cells). (ii) A pyramidal‐shaped neuron a cell body located...
The Ca 2+ /calmodulin-dependent protein phosphatase 2B or calcineurin (CN) participates in several -dependent signal transduction cascades and, thus, contributes to the short and long term regulation of neuronal excitability. By using a specific antibody CN, we demonstrate its absence from hippocampal interneurons illustrate physiological consequence such CN deficiency. Consistent with lack as detected by immunocytochemistry, inhibitors FK-506 okadaic acid significantly prolonged N -methyl-...
The activity of Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) plays an integral role in regulating synaptic development and plasticity. We designed a live-cell-imaging approach to monitor activity-dependent clustering green fluorescent (GFP)-CaMKII holoenzymes, termed self-association, process that we hypothesize contributes the translocation CaMKII nonsynaptic sites activated neurons. show GFP-CaMKII self-association human embryonic kidney 293 (HEK293) cells requires catalytic...
The serotonergic system plays a key role in the regulation of brain states, and many known features neurons appear to match this function. Midbrain raphe nuclei provide diffuse projection all regions forebrain, exhibit slow metronome-like activity that sets ambient levels serotonin across sleep–wake cycle. Serotonergic cells have also been implicated, however, variety more specific functions can hardly be related their low-rate monotonous patterns discharges. amazing receptors type-specific...
Intermittently occurring field events, dentate spikes (DS), and sharp waves (SPW) in the hippocampus reflect population synchrony of principal cells interneurons along entorhinal cortex-hippocampus axis. We have investigated cellular-synaptic generation DSs SPWs by intracellular recording from granule cells, pyramidal anesthetized rats. The recorded neurons were anatomically identified injection biocytin. Extracellular electrodes placed hilus to record multiple units CA1 cell layer monitor...
Dendritic morphology and passive cable properties determine many aspects of synaptic integration in complex neurons, together with voltage-dependent membrane conductances. We investigated dendritic CA1 pyramidal neurons intracellularly labeled during vivo vitro physiologic recordings, by using similar intracellular staining three-dimensional reconstruction techniques. Total length the was to that cells. After correction for shrinkage, cell extent representation not different between two...
Abstract The complete axon arborization of a single CA3 pyramidal cell has been reconstructed from 32 (60 /μm thick) sections the rat hippocampus following in vivo intracellular injection neurobiotin. same were double‐immunostained for parvalbumin‐a calcium‐binding protein selectively present two types GABAergic interneurons, basket and chandelier cells‐in order to map boutons contact with dendrites somata these specific subsets interneurons visualized Golgi‐like manner. formed 15 295...
Abstract In order to produce its desired effect, anaesthesia acts upon neuronal elements by modifying membrane conductances and transmitter interactions. The effect of higher doses isoflurane, widely used in clinical settings, on the permeability blood–brain barrier (BBB) is meanwhile ignored. this study we investigated integrity BBB during various levels isoflurane (1% 3%) cats monitoring extravasation Evans blue. Simultaneously measured electroencephalogram (EEG), with particular emphasis...
Abstract Recent electrophysiological studies have discovered evidence of heterogeneity 5‐hydroxytryptamine (5‐HT) neurons in the mesencephalic raphe nuclei. Of particular interest is a subpopulation putative 5‐HT that display many properties presumed 5‐HT‐containing (regular and slow firing single spikes with broad waveform) but fire short, stereotyped bursts. In present study we investigated chemical identity these rats utilizing vivo juxtacellular labelling methods. ten dorsal nucleus...
Abstract Ipsilateral and contralateral hippocampal CA3–CA1 CA2–CA1 projections were investigated in adult male Long–Evans rats by retrograde tracing. Injection of the tracer cholera toxin subunit B strata oriens radiatum dorsal CA1 resulted labeling predominantly pyramidal cells ipsilateral CA3 CA2. The anterior–posterior extents innervation to similar. Fifteen twenty per cent hippocampus proper that give rise stratum CA2 cells, whereas a mere 3% for innervation. preferred projection was...