In the realm of nanotechnology, use algae to produce nanoparticles is an environmentally friendly, sustainable, and economically viable strategy. present study, brown macroalgae Sargassum subrepandum was utilized effectively silver (AgNPs). Through various characterization techniques, AgNPs’ structural integrity confirmed. AgNPs exhibited significant antimicrobial activity against Pseudomonas aeruginosa Fusarium equiseti . showed cytotoxic effects on MCF-7 breast adenocarcinoma cell line...

Abstract Microalgae have received continued attention as a potential source for biofuel production. However, the lack of suitable strains that provide lipid‐rich biomass and tolerate harsh condition inhibits their industrial application. This report describes an effort to transform Synechocystis sp. with genes encoding acetyl‐CoA carboxylase (ACC), key regulatory enzyme in lipogenesis pathway, from white mustard plant ( Sinapis alba ) bacterium Escherichia coli DH5α using chitosan...

Microalgae have garnered scientific interest for their potential to produce bioactive compounds. However, the large-scale industrial utilization of microalgae faces challenges related production costs and achieving optimal growth conditions. Thus, this study aimed investigate role exogenous indole-3-acetic acid (IAA) application in improving metabolites microalgae. To end, employed different concentrations exogenously administered IAA ranging from 0.36 µM 5.69 assess its influence on...

Two thionin genes were isolated from Arabidopsis thaliana (AT1G12660 and AT1G12663).The resulted protein has antifungal activity against potato pathogenic fungi like Alternaria alternata Rhizoctonia solani.These two respectively inserted in pEGAD vector.After that carried into chitosan nanoparticle order to be transformed the tested cultivars (lady spunta) using intermodal cutting technique of tissue culture.Expressions transgenes transgenic plants confirmed by RT-PCR.There assays applied...

Background: Biofuels produced from trans-esterification of high lipid content in microalgae represent a promising alternative renewable source energy to the limited and depleted global fossil fuel reservoir. The most critical step such process is harvesting algal cells. Objective: We aimed improve current methodology for via utilizing biosynthesized silver nanoparticles (AgNPs) Synechocystis sp. ElfSCS31 as an eco-friendly, stable, affordable flocculant agent. Methods: AgNPs were prepared by...

Paulownia tomentosa (Thunb.) Steud. is a very important hard woody plant, an extremely fast-growing tree and produce timber. Therefore, there demand to transgenic plant resistant bacterial infection. Microbial infection (especially one) serious sever cause loss in productivity as they bear upon the character amount of product. Two phytopathogenic bacteria were chosen consider their effect on tomentosa. These two species Erwinia carotovora Pseudomonas aeruginosa. thionin genes (AT1G12660...

Abstract This study evaluated the lycopene content in raw tomato varieties and processed products. The separation of isomers was conducted by high‐performance liquid chromatography. Lycopene determined on a dry weight basis (DWB). function three different (cis, all‐trans 5‐cis) examined. Data for were analyzed within among groups. Cherry tomatoes ranked highest content. On DWB, Roma contained concentration, while vine lowest Among products, paste canned juice lowest. Furthermore, catsup...

The potential of microalgae to produce valuable compounds has garnered considerable attention. However, there are various challenges that hinder their large-scale industrial utilization, such as high production costs and the complexities associated with achieving optimal growth conditions. Therefore, we investigated effects glycine at different concentrations on bioactive Synechocystis sp. PAK13 Chlorella variabilis cultivated under nitrogen availability. Glycine supplementation resulted in...

Aspergillus niger is a dangerous pathogen for many plants. It major cause of the destruction, rotting and decomposition plant tissues. Toxicity caused by A. can be inhibited mutation decreasing destructive effect on An 18S rDNA molecular tool was used to identify strains. Sodium azide (NaN3) chemical mutagen that disturbs fungal enzymatic activity causes microbial production cellulose-degrading enzymes, mycotoxin production. Different concentrations sodium were treat (30, 40 50 µM). The...

Abstract The date palm, or Phoenix dactylifera L., is one of the world’s oldest fruit trees. entire palm tree used to produce a variety goods, including food, clothing, fibre, and shelter. Tissue culture most recent methods multiply palms disease-free offspring. Plant tissue technology has advantages over conventional propagation for quick extensive multiplication significant plants in vitro, regardless season, disease-free. This because it preserves space time. For this study, three...

In the realm of applied phycology, algal physiology, and biochemistry publications, absence proper identification documentation microalgae is a common concern. This poses significant challenge for non-specialists who struggle to identify numerous eukaryotic microalgae. However, promising solution lies in employing an appropriate DNA barcoding technique establishing comprehensive databases reference sequences. To address this issue, we conducted study focusing on molecular characterization...

Two thionin genes were isolated from Arabidopsis thaliana (AT1G12660 and AT1G12663).The resulted protein has antifungal activity against potato pathogenic fungi like Fusariumsolani Fusariumoxysporum.These two respectively inserted into pEGAD vector.After that transformed Agrobacterium tumifaceins GV3101::pMP90 in order to be the cultivars (lady spunta) using nodal cutting technique of tissue culture.Expressions transgenes transgenic plants confirmed by RT-PCR.There assays applied test role...

Calla lily (Zantedeschia albomaculata (Hook.) Baill.) is an herbaceous or semi-evergreen perennial grown from rhizomes. It commonly named "Spotted Arum". Ribosomal RNAs (rRNAs) are found in all known organisms and for being functionally equivalent of them. A completely new vitro culture protocol was applied to Z. with two hormones, 6-Benzylaminopurine (BAP) kinetin, obtain full growth multiplication. Due their highly conserved sequences, the analysis small-subunit rRNAs (16S-18S rRNAs) can...

In vitro culture growth rate of Populus alba upon using nano metal-based-oxides such as hematite (Fe 2 O 3 NPs), zinc oxide (ZnO and manganese (Mn NPs) nanoparticles micronutrients.

Abstract Fenugreek is a globally important legume that widely cultivated for its therapeutic benefits in most parts of the world. Seeds on other hand have poor germination and growth rate when exposed to salinity. The effect ultrasonic exposure period early seedling behaviors fenugreek seeds under salt stress was investigated laboratory experiment. During stages, all tests were conducted at 40 kHz water bath device with two durations (10 20 min) salinity using different concentrations NaCl...

The chemical mutagens, namely sodium azide and guanidine hydrochloride (30, 40 50 mM) for each were applied the in vitro culture of Gardenia jasminoides Ellis. Eight morphological traits evaluated to calculate result different concentrations mutagens compared control. In azide, all decreased control, except number branches leaves leaf length, which increases Unlike hydrochloride, are enhanced mutants rather than polymorphism percentage resulted from genetic variation varied (57.71% case...

The utilization of chitosan nanoparticles is a novel technique for gene transformation into plant tissues. It takes few minutes to transform plant. UidA was detected in <i>Escherichia coli</i> (K12 strain) using polymerase chain reaction analysis by UidA-specific primers. transformed the explants two different species (<i>Solanum tuberosum</i> and <i>Paulownia tomentosa</i>). These plants have natures as crop woody respectively. Therefore, they abilities...