Bovine papillomaviruses (BPVs) are widespread pathogens mainly associated with benign, self-limiting, cutaneous lesions (warts). At least 8 viral types, defined by serology or nucleotide sequences of the L1 gene, have been identified to date. Different serotypes specific type and morphology lesion particular geographical regions. This article describes molecular identification from Brazilian cattle (n = 48) horses 1) through partial amplification sequencing gene. papillomavirus-1 (BPV-1) was...

ABSTRACT: Pregnancy diagnosis is an important tool for farm management. Ultrasonography the main technique used pregnancy in ewes. As alternative, radioimmunoassay (RIA) allows accurate and early detection of pregnancy-associated glycoproteins (PAGs) sheep blood. However, radioactive-based techniques, as RIA, have been increasingly inadvisable due to environmental risk. Homology between ovine bovine PAGs high, ELISA kits cattle are safer than RIA. Thus, this study aimed evaluate feasibility...

The aim of this study was to evaluate secondary clinical disease, milk production efficiency and reproductive performance heifers cows persistently infected (PI) with bovine viral diarrhea virus type 2 (BVDV 2). PI animals (n = 25) were identified using an antigen capture ELISA ear notch samples. They distributed into three age groups: ≤ 12 8), 13 24 6) 25 34 11) months old. A control group BVDV negative female cattle that matched the utilized from same herd. had a 1.29 higher odds ratio for...

Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses capable establishing latent infections in neuronal tissues. Infected animals tend develop a serological response infection; however, such response—usually investigated by antibody assays serum—may eventually not be detected laboratory assays. Nevertheless, tests as virus neutralization (VN) various enzyme-linked immunosorbent (ELISAs) widely...

Anticorpos monoclonais (AcMs) produzidos com uma amostra brasileira do herpesvírus bovino tipo 5 (BHV-5) foram utilizados na adaptação e padronização de técnicas rápidas para o diagnóstico infecções pelo BHV-1 BHV-5. A detecção antígenos virais por imunofluorescência em células descamativas (IFCD) foi comparada isolamento viral, secreções nasais 16 bezerros, sendo 6 inoculados 10 De 203 amostras testadas, 182 (89,6%) apresentaram resultados concordantes nos dois testes (143 positivas; 39...

O herpesvírus bovino tipo 2 (BoHV-2), agente da mamilite herpética bovina (BHM), possui distribuição mundial e foi identificado no Brasil, na década de 70. A partir então, casos clinicamente compatíveis com a BHM têm sido relatados por técnicos produtores, o que sugere presença disseminação do rebanho brasileiro. Este trabalho relata um inquérito sorológico infecção pelo BoHV-2 Estado Rio Grande Sul (RS), em amostras coletadas como parte Programa Nacional Controle Erradicação Brucelose...

Bovine herpetic mammillits is a self-limiting cutaneous disease of the udder and teats cows associated with bovine herpesvirus 2 (BoHV-2) whose pathogenesis poorly understood. This article describes use guinea pigs (Cavia porcellus) to study BoHV-2 infection. Twelve weanling female inoculated subcutaneously in genitalia developed local hyperemia, edema, vesicles, ulcers scabs. Infectious virus was recovered between days 3 7 post-infection (pi) from genital area (9/12) (1/12); all animals...

We wished to determine if Mycoplasma bovis infection can negatively impact milk quality and production in Holstein dairy cows. For this Research Communication, samples (271) from cows 3 herds were screened for M. by real-time PCR. Positive (n = 21) negative animals matched herd, age, lactations days (DIM). Pairs evaluated 7 stages of lactation: D1-50, D51-100, D101-150, D151-200, D201-250, D251-300, D ≥ 301. A mixed model was used assess the effect groups (M. bovis+ × M.bovis-), time...

ABSTRACT: This study aimed to evaluate the effect of bovine leukemia virus (BLV) on systemic profile naturally infected dairy heifers during transition period. Pregnant Holstein and Jersey (n=24) were distributed in pairs into two experimental groups: (BLV+) (BLV-). Animals BLV+ group divided subgroups based median BLV proviral load (high low). The animals then assessed at weeks -3, -2, -1, calving time (0), +1, +2, +3. Blood samples obtained for hematological biochemical analyses, as well...

The negative effect of some diseases, such as bovine viral diarrhea (BVD), infectious rhinotracheitis (IBR), and Leptospirosis, on reproduction rates are well known. uses vaccines considered to be an important tool available in order control reproductive losses but their efficiency is still controversial. aim this study was evaluate the effects vaccination against BVD, IBR, Leptospirosis improve pregnancy rate beef cattle submitted fixed-timed AI (FTAI). Nelore cows (n = 1172) from 4 farms...

Infectious diseases can directly impact the reproductive system (germ cells transport, uterine environment, embryo/fetus) or indirectly affect embryonic/fetal survival (uterine and placental dysfunction).In this document, main infectious caused by viruses, bacteria protozoa that performance most prevalent today in Brazil will be discussed, considering diagnoses information obtained so far.In addition, topics for assertiveness of diagnosis pointed, such as importance establishing a diagnostic...

A Nanometrologia é uma parte da metrologia que trata das medidas de diversas grandezas ao nível escala nanométrica. Com o avanço exponencial nanotecnologia nas últimas décadas, foi exigido um empenho enorme dos Laboratórios Nacionais Metrologia para acompanhar esse avanço, oferecendo meios garantir a rastreabilidade metrológica demandada por esta área. O presente artigo tem intenção apresentar retrato situação nanometrologia no Brasil e mundo, meio levantamento dados disponibilizados pelos...