A5082256484- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Classical Philosophy and Thought
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Historical Economic and Social Studies
- South Asian Studies and Conflicts
- Epistemology, Ethics, and Metaphysics
- Academic and Historical Perspectives in Psychology
- Logic, Reasoning, and Knowledge
- Seismic Imaging and Inversion Techniques
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Agricultural Economics and Practices
- Philosophy and History of Science
- Molecular Biology Techniques and Applications
- Paranormal Experiences and Beliefs
- Bacterial Genetics and Biotechnology
- Nuclear Structure and Function
- Philosophy and Theoretical Science
- PARP inhibition in cancer therapy
- Single-cell and spatial transcriptomics
- Medical Education and Admissions
University of Lisbon
2012-2025
Instituto Gulbenkian de Ciência
2025
Saskatchewan Health
2024
Thomas Jefferson University
2019
Instituto Politécnico de Lisboa
2015
Fundação Faculdade de Medicina
2015
Instituto de Medicina Molecular João Lobo Antunes
2015
Department of Water
2012
Curtin University
2012
Max Delbrück Center
2005-2009
Published with the aid of a grant from National Endowment for Humanities. Contains only complete English-language text The Concept Truth in Formalized Languages. Tarski made extensive corrections and revisions original translations this edition, along new historical remarks. It includes preface analytical index use by philosophers linguists as well historians mathematics philosophy.
Cell-penetrating peptides (CPPs) are capable of introducing a wide range cargoes into living cells. Descriptions the internalization process vary from energy-independent cell penetration membranes to endocytic uptake. To elucidate whether mechanism entry CPP constructs might be influenced by properties cargo, we used time lapse confocal microscopy analysis mammalian cells directly compare uptake well-studied TAT fused protein (>50 amino acids) or peptide (<50 cargo. We also analyzed various...
Abstract Cell penetrating peptides (CPPs) are useful tools to deliver low‐molecular‐weight cargoes into cells; however, their mode of uptake is still controversial. The most efficient CPPs belong the group arginine‐rich peptides, but a systematic assessment potential toxicity lacking. In this study we combined data on membrane translocation abilities oligo‐arginines in living cells as function chain length, concentration, stability and toxicity. Using confocal microscopy analysis evaluated...
Abstract Background Live cell fluorescence microscopy experiments often require visualization of the nucleus and chromatin to determine nuclear morphology or localization compartments. Methods We compared five different DNA dyes, TOPRO‐3, TOTO‐3, propidium iodide, Hoechst 33258, DRAQ5, test their usefulness in live with continuous imaging photobleaching widefield epifluorescence confocal laser scanning microscopy. In addition, we stainings fluorescent histones as an independent label mark...
Removal of introns from pre-messenger RNAs (pre-mRNAs) via splicing provides a versatile means genetic regulation that is often disrupted in human diseases. To decipher how occurs real time, we directly examined with single-molecule sensitivity the kinetics intron excision pre-mRNA nucleus living cells. By using two different RNA labeling methods, MS2 and λN, show β-globin are transcribed excised 20–30 s. Furthermore, replacing weak polypyrimidine (Py) tract mouse immunoglobulin μ (IgM) by...
The nucleolus is the hallmark of nuclear compartmentalization and has been shown to exert multiple roles in cellular metabolism besides its main function as place rRNA synthesis assembly ribosomes. Nucleolar proteins dynamically localize accumulate this compartment relative surrounding nucleoplasm. In study, we have assessed molecular requirements that are necessary sufficient for localization accumulation peptides inside nucleoli living cells. data showed positively charged peptide entities...
Genome activity and nuclear metabolism clearly depend on accessibility, but it is not known whether to what extent structures limit the mobility access of individual molecules. We used fluorescently labeled streptavidin with a localization signal as an average-sized, inert protein probe environment. The was injected into cytoplasm mouse cells, single molecules were tracked in nucleus high-speed fluorescence microscopy. analyzed compared structurally functionally distinct compartments living...
The condensation level of chromatin is controlled by epigenetic modifications and associated regulatory factors changes throughout differentiation cell cycle progression. To test whether levels per se affect access binding proteins, we used a hypertonic treatment. This shift to hyperosmolar medium increased nuclear calcium concentrations induced reversible comparable the in mitosis. However, this was independent mitotic histone H3 serine 10 phosphorylation. Photobleaching photoactivation...
DNA damage within active genes drives bidirectional break-induced transcription initiation.
A social psychology or generalized conclusion based on experimental investigations of freshmen sophomores in college is highly debatable. Use volunteers from such sophomore classes even more debatable for it may represent a selection within selection. In work also wise precaution to know as many characteristics possible the group being investi
Abstract While DNA:RNA hybrids contribute to multiple genomic transactions, their unscheduled formation is a recognized source of DNA lesions. Here, through suite systematic screens, we rather observed that wide range yeast mutant situations primarily triggering damage actually leads hybrid accumulation. Focusing on Okazaki fragment processing, establish genic can form as consequence replication-born discontinuities such unprocessed flaps or unligated fragments. Strikingly, “post-lesion”...
A flow cytometer has been constructed which measures total fluorescence and the distribution of along isolated, stained mammalian chromosomes. In this device, chromosomes lengthwise at 4 m/sec through a 1-micrometer thick laser beam. The from each chromosome is recorded 10 nsec intervals; sequence values represents stored in memory waveform recorder. also measured recorded. Preliminary studies show that doublets 1.83 micrometers diameter microspheres with their long axes parallel to...
Abstract We have developed RHINO, a genetically encoded sensor that selectively binds RNA:DNA hybrids enabling live-cell imaging of cellular R-loops. RHINO comprises tandem array three copies the hybrid binding domain human RNase H1 connected by optimized linker segments and fused to fluorescent protein. This tool allows measurement R-loop abundance dynamics in live cells with high specificity sensitivity. Using we provide kinetic framework for R-loops at nucleoli, telomeres protein-coding...
Nonviral jet injection is an applicable technology for in vivo gene transfer of naked DNA. However, little known about the biodistribution and clearance jet-injected DNA, or its localization within tissue cells. Therefore, this study we analyzed intratumoral systemic DNA human colon carcinoma-bearing NCr-nu/nu mice, which were with pCMVβ plasmid Intratumoral was 5, 10, 20, 40 min 3, 6, 24, 48, 72 hr after injection, using quantitative real-time polymerase chain reaction. In tumors, a rapid...