The dynamics of Xylella fastidiosa infections in the context endophytic microbiome was studied field-grown plants susceptible and resistant olive cultivars Kalamata FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene carried out on same trees at two different stages infections: In Spring 2017 when were almost symptomless Autumn 2018 cultivar clearly showed desiccations. progression detected both unraveled that tends to occupy whole ecological niche suppresses...

Huanglongbing (HLB), commonly known as citrus greening disease, is among the most severe diseases affecting Citrus genus. It caused by three species of Candidatus Liberibacter bacterium: asiaticus (Las), africanus (Laf), and americanus (Lam). This study aimed to investigate presence HLB in various varieties exhibiting suspected symptoms within gene bank Suhar, Oman. Samples were collected subjected PCR amplification using two primer sets, CN265/CN266 OI1/OI2c, followed restriction enzyme...

Abstract Grapevine leafroll disease is the most damaging viral afflicting global grape and wine production. Of five viruses likely associated with disease, grapevine leafroll-associated virus 3 (GLRaV3) believed to be predominant agent, albeit its role as causal agent has remained uncertain. GLRaV3 (species Ampelovirus trivitis, genus Ampelovirus, family Closteroviridae) third largest single-stranded, positive-sense RNA genome among plant at ~18.5 kb, only surpassed by two other members of...

Management of Xylella fastidiosa subsp. pauca (Xfp) presents significant challenges due to the lack genetic resistance in major crops, pathogen variability and development against main antimicrobial compounds, effective chemical compounds for control. Traditional bactericides EU are restricted, copper-based products increasingly limited environmental impacts. In this study antibacterial side effects some commercial fertilizers Xfp were evaluated. These products, containing metal ions (mainly...

Abstract Xylella fastidiosa subspecies pauca strain De Donno (XfDD) ST53 is the causal agent of olive quick decline syndrome, a severe disease first described in Apulia, Italy. Although two local cultivars Cellina di Nardò and Ogliarola Salentina showed high susceptibility, traits resistance to bacterium were found cultivar Leccino. Previous studies field‐grown olives suggested that vascular occlusions anatomophysiological properties different played role response XfDD. The present...

Olive quick decline syndrome (OQDS) is a severe disease, first described in Italy late 2013, caused by strains of Xylella fastidiosa subsp. pauca (Xfp) susceptible olive cultivars. Conversely, resistant cultivars do not develop OQDS but present scattered branch dieback, which generally does evolve to canopy decline. In the study, we assessed physiological responses Xfp -infected trees and Periodic measurements stomatal conductance (g s ) stem water potential (Ψstem) were performed using set...

Abstract Citrus hosts various phytopathogens that have impacted productivity, including viroids. Missing data on the status of viroids in citrus Palestine were not reported. This study was aimed to detect any exocortis viroid (CEVd), viroid-III (CVd-III), and viroid-IV (CVd-IV) Palestinian National Agricultural Research Center (NARC) germplasm collection Field inspections found symptoms such as leaf epinasty; vein discoloration, bark cracking varieties. RT-PCR revealed a significant...

Abstract In Morocco, the climate conditions are favorable for establishment and spread of Xylella fastidiosa (X.f) . However, successful bacterium depends on many factors; mainly: bacterial subspecies sequence type, host plants susceptibility, feeding behavior transmission efficiency insect vectors. Knowledge relationship between bacterium–affected crop agro-ecosystem potential vectors is crucial importance. this work, we list tritrophic interaction X.f -host plants-insect that occur...

Introduction The epidemic spread of the harmful bacterium Xylella fastidiosa causing “olive quick decline syndrome”, decimating olive trees in southern Italy, region Apulia, prompted investigations to search for genotypes harbouring traits resistance. Methods A prospecting survey was carried out identify, heavily infected area bearing Given limited genetic diversity commercial groves with few cultivars widely cultivated, surveys targeted predominantly spontaneous natural and uncultivated...

The recent finding of Xylella fastidiosa ( Xf ) in olive trees southern Italy, the scanty molecular information on this bacterium and its association with quick decline syndrome (OQDS) prompted necessity to isolate acquire more genetic data type strain present that region. For first time, was isolated from infected culture media. Genetic were obtained through genomic comparison other subspecies or strains. sequences thirteen genes genome, comprising seven housekeeping leuA, petC, lacF ,...

Abstract The presence of Hop stunt viroid (HSVd) was detected using RT‐PCR and Northern blot hybridization in five 60 samples from symptomless mulberry trees ( Morus alba ) collected Italian Lebanese orchards July 2010. Infection levels were c. 10% 8% samples. Nucleotide alignments showed that sequences the HSVd isolates shared 95–96% identity with those same occurring elsewhere. In a phylogenetic tree, clustered together HSVd‐citrus, regardless their geographical origin. This is first...

Since its outbreak in 2013 Italy, the harmful bacterium Xylella fastidiosa has continued to spread throughout Euro-Mediterranean basin and, more recently, Middle East region. subsp. is causal agent of Pierce’s disease on grapevines. At present, this alien subspecies not been reported Lebanon but if biological invader was with no cost-effective and sustainable management, it would put Lebanese vineyards at a certain level risk. In absence an outbreak, gross revenue generated by wine growers...

Climate and trade changes are reshaping the cartographic distribution of lethal pervasive pathogens. Among serious emerging challenges is Xylella fastidiosa (Xf), a xylem-limited phytopathogenic bacterium that produces losses damages to numerous crops high economic agronomic importance. Lately, this grave quarantine pathogen has expended its by arriving several European countries infecting both wild cultivated plants, no cure been identified so far. Countries without current outbreaks like...

Tomato (Solanum lycopersicum) plants from a commercial glasshouse were identified with symptoms compatible tomato brown rugose fruit virus (ToBRFV) infection. Reverse transcription-PCR and quantitative PCR confirmed the presence of ToBRFV. Subsequently, same RNA sample second infected similar tobamovirus, mottle mosaic (ToMMV), extracted processed for high-throughput sequencing Oxford Nanopore Technology (ONT). For targeted detection ToBRFV, two libraries synthesized by using six ToBRFV...

RT-PCR amplicon was sequenced in both directions, and the sequence deposited GenBank as accession number OR501605.Bioinformatics BLAST analysis of showed 100% nucleotide identity with ToBRFV isolates from USA, Turkey Netherland (MT002973, MT107885 MN882058, respectively).Very likely, introduced to Uzbekistan on imported 'Alamina' seeds that were infected.To our knowledge, this is first report Uzbekistan.

An outbreak of Xylella fastidiosa was discovered in late 2018 northern Italy affecting several plant species. Multilocus sequence typing analyses detected the presence strains clustering X. subsp. multiplex and harboring a hitherto uncharacterized type, ST87. Three cultured (TOS4, TOS5, TOS14) were subjected to high-throughput sequencing draft genomes assembled. Phylogenetic analysis conclusively indicated that they belong subspecies multiplex. The genetic information generated for these...

Diseases caused by Xylella fastidiosa are among the most destructive for several agricultural productions. A deadly disease of olive, termed olive quick decline syndrome, is one recent examples severe impacts introduction and spread this bacterium in new ecosystems with favorable epidemiological conditions. Deciphering cascade events leading to development alterations susceptible host plants a priority research programs investigating strategies mitigate detrimental infections. However, case...

This research was carried out in order to evaluate the presence and distribution of viral infections causing severe disease eggplant plants collected from different districts Bekaa valley, Lebanon. Most infected showed virus-like symptoms consisting predominantly leaf blotch, mottling chlorotic ring spots; twisting plant dwarf were also observed visited fields. Symptomatic asymptomatic screened by ELISA test for several pathogenic viruses potentially present area. Results that potato virus Y...