- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Mosquito-borne diseases and control
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Resistance
- Advances in Cucurbitaceae Research
- Plant Disease Management Techniques
- Viral Infections and Vectors
Benaki Phytopathological Institute
2013-2024
Agricultural University of Athens
2007
Quinone outside inhibitors (QoI) are powerful fungicides, which have been reported, additionally to their fungicide activity, increase plant capacity activate cellular defense responses and promote growth. In this work, the effect of QoI class pyraclostrobin was examined against Cucumber mosaic virus (CMV), Potato Y (PVY) Pseudomonas syringae pv. tomato in plants following artificial inoculation with pathogens. Under controlled environmental conditions, delayed viral bacterial disease...
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), stem pitting (ASPV) and grooving (ASGV) in total RNA pome trees extracted with a CTAB method. The sensitivity method established using vitro synthesized viral transcripts serially diluted from healthy, virus-tested (negative) trees. three viruses were simultaneously detected up to 10−4 dilution naturally...
Citrus impietratura disease (CID) is a graft transmissible, virus-like observed in old-line citrus trees; its characteristic symptom the appearance of gum albedo affected fruits. To identify causal agent disease, high-throughput sequencing (HTS) was performed on symptomatic orange The analysis obtained data revealed all samples mixed infections viroids commonly found trees together with recently described virus A (CiVA). Examination additional fruits conventional reverse transcription PCR...
In the summer of 2012, zucchini (Cucurbita pepo L.) plants F1 hybrid Rigas showing very severe malformation and blisters in leaves fruit were observed prefectures Ilia Messinia, Peloponnese, southwestern Greece. Over 100 samples collected only a few found by double antibody sandwich (DAS)-ELISA to be singly or mixed infected with commonly encountered Cucumber mosaic virus (CMV, genus Cucumovirus), Zucchini yellow (ZYMV, Potyvirus), Watermelon (WMV, which is known tolerant. All affected also...
Potato spindle tuber viroid (PSTVd) has quarantine status in the European Union. During 2009, a survey of PSTVd ornamental plants species Solanum jasminoides and Brugmansia spp. was conducted Greece. Samples were collected from 21 commercial nurseries located central, north, south west Greece, consisted symptomless upper leaves at random 10% within each nursery. A total 391 samples (315 S. 76 spp.) examined. Total RNA extracted sample analysed by reverse transcription polymerase chain...
Citrus tristeza virus (CTV) has quarantine status in Greece. The was first reported 2000 non-certified sweet orange trees grafted on CTV-tolerant Carrizo citrange originating from Spain (Dimou et al., 2002). High homology of these isolates then found with the Spanish mild T385 isolate (bio-group 1, Garnsey 2005) sour rootstock, sharing 100% nucleotide sequence identity for p20 gene (Varveri, 2006). In 2009, surveys were conducted area Poros (Trizinia, Prefecture Pireaus), where about 20,000...
In summer 2023, a sample of clingstone peach (Prunus persica cv. PI-A37) from Ampeleies (regional unit Pella, Greece) exhibiting virus-like symptoms, i.e., mosaic and chlorotic spots on the leaves, bud failure severe fruit deformity, was analysed with High Throughput Sequencing (HTS). Total RNA extracted leaves an RNeasy Plant Mini Kit (Qiagen, Germany) subjected to RNA-Seq in Illumina Novaseq 6000 platform. The HTS analysis resulted approximately 40 million, paired-end 150 bp reads that...
During winter 2020-2021, a severe virus-like disease outbreak was observed in eggplant (