- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Insect and Pesticide Research
- Plant Disease Management Techniques
- Plant-Microbe Interactions and Immunity
- Viral Infections and Vectors
Benaki Phytopathological Institute
2011-2024
Aphis gossypii (Sternorrhyncha: Aphididae) aphids are vectors of important plant viruses among which cucumber mosaic virus (CMV) and potato Y (PVY). Virus-infected plants attract aphid affect their behavior growth performance either positively or negatively depending on mode transmission. Viruses cause changes in the composition amount volatile organic compounds (VOCs) released by that aphids. The parasitoid Aphidius colemani (Hymenoptera: Aphelinidae) has been shown to have higher...
Citrus impietratura disease (CID) is a graft transmissible, virus-like observed in old-line citrus trees; its characteristic symptom the appearance of gum albedo affected fruits. To identify causal agent disease, high-throughput sequencing (HTS) was performed on symptomatic orange The analysis obtained data revealed all samples mixed infections viroids commonly found trees together with recently described virus A (CiVA). Examination additional fruits conventional reverse transcription PCR...
In autumn 2022, a tomato sample (Solanum lycopersicum, cv. Ekstasis F1 hybrid) from Aspropyrgos, Attiki, Greece, infected with Tomato brown rugose fruit virus (ToBRFV) was analysed RNA-Seq. Total RNA isolated leaves an RNeasy Plant Mini Kit (Qiagen, Germany) and subjected to RNA-Seq in Illumina Novaseq 6000 platform. The BLASTn annotation of the contigs obtained analysis 12 M, single-end, 100 bp reads Geneious (v. 11.1.5), revealed presence ToBRFV, Southern (genus Amalgavirus), already...
In winter 2023 fruit blotch symptoms (Beris et al., 2023) were observed in tomato samples (Solanum lycopersicum cv. Esperia F1 hybrid) from a greenhouse Ierapetra (Crete, Greece). To identify the causal agent of disease, two individually analysed with RNA-Seq. Total RNA was extracted an RNeasy Plant Mini Kit (Qiagen, Germany) leaf and tissues after ribodepletion, it using Illumina Novaseq 6000 platform. The analysis obtained 30 M, paired-end, 100 bp reads Geneious (v. 11.1.5) subsequent...
In winter 2023, tomato fruit (Solanum lycopersicum cv. Esperia F1 hybrid) from a greenhouse in Ierapetra, Crete, Greece exhibited blotches at rate of 7%. The plants had previously tested positive by RT-PCR for TFBV (Beris et al., 2023) but sample was analysed with RNA-Seq to investigate the aetiology disease. Total RNA extracted leaf and tissue an RNeasy Plant Mini Kit (Qiagen, Germany) after ribodepletion, subjected using Illumina Novaseq 6000 platform. obtained 30 M, paired-end, 100 bp...
During winter 2020-2021, a severe virus-like disease outbreak was observed in eggplant (