A5000672530- Oral microbiology and periodontitis research
- Salivary Gland Disorders and Functions
- Oral Health Pathology and Treatment
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Oral and gingival health research
- HIV/AIDS oral health manifestations
- Dental Health and Care Utilization
- Streptococcal Infections and Treatments
- Vitamin D Research Studies
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- RNA modifications and cancer
- Infective Endocarditis Diagnosis and Management
- Stress Responses and Cortisol
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Microbial Fuel Cells and Bioremediation
University of Florida
2017-2024
Florida College
2018-2024
Harvard University Press
2015
Novant Health Forsyth Medical Center
2007
The complexity of the human microbiome makes it difficult to reveal organizational principles community and even more challenging generate testable hypotheses. It has been suggested that in gut species such as Bacteroides thetaiotaomicron are keystone maintaining stability functional adaptability microbial community. In this study, we investigate interspecies associations a complex biofilm applying systems biology principles. Using correlation network analysis identified bacterial modules...
ABSTRACT Oral bacterial biofilms are highly complex microbial communities with up to 700 different taxa. We report here the use of metatranscriptomic analysis study patterns community gene expression in a multispecies biofilm model composed species found healthy oral ( Actinomyces naeslundii , Lactobacillus casei Streptococcus mitis Veillonella parvula and Fusobacterium nucleatum ) same plus periodontopathogens Porphyromonas gingivalis Aggregatibacter actinomycetemcomitans . The presence...
Abstract Imbalances of the microbiome, also referred to as microbial dysbiosis, could lead a series different diseases. One factor that has been shown dysbiosis microbiome is exposure psychological stressors. Throughout evolution microorganisms human have developed systems for sensing host-associated signals such hormones associated with those stressors, enabling them recognize essential changes in their environment, thus changing expression gene profile fit needs new environment. The most...
Dysbiosis, or the imbalance in structural and/or functional properties of microbiome, is at origin important infectious inflammatory diseases such as bowel disease (IBD) and periodontal disease. Periodontitis a polymicrobial that affects large proportion world's population has been associated with wide variety systemic health conditions, diabetes, cardiovascular respiratory diseases. Dysbiosis identified key element development However, precise mechanisms environmental signals lead to...
ABSTRACT Oral microbial communities are extremely complex biofilms with high numbers of bacterial species interacting each other (and the host) to maintain homeostasis system. Disturbance in oral microbiome can lead either caries or periodontitis, two most common human diseases. Periodontitis is a polymicrobial disease caused by coordinated action community, which results inflammation tissues that support teeth. It cause tooth loss among adults United States, and recent studies have...
Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has small number two-component signal transduction systems, and after comparing genome sequences strains W83 ATCC 33277 we discovered that latter was mutant in histidine kinase (PGN_0752), while cognate response regulator (PGN_0753) remained intact. Microarray-based transcriptional profiling ChIP-seq assays were carried out an transconjugant containing functional from strain...
ABSTRACT The oral microbiome is one of the most complex microbial communities in human body, and due to circumstances not completely understood, healthy community becomes dysbiotic, giving rise periodontitis, a polymicrobial inflammatory disease. We previously reported results community-wide gene expression changes during periodontitis progression identified signatures associated with increasing severity Small noncoding RNAs (sRNAs) are key players posttranscriptional regulation, especially...
Abstract Background Oral microbiome dysbiosis is linked to overt inflammation of tooth-supporting tissues, leading periodontitis, an oral condition that can cause tooth and bone loss. Microbiome has been described as a disruption in the symbiotic microbiota composition’s stability could adversely affect host’s health status. However, precise dynamics lead progression disease are largely unknown. The objective our study was investigate long-term periodontitis its connection dysbiosis. Results...
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas system is a unique genomic entity that provides prokaryotic cells with adaptive and heritable immunity. Initial studies identified CRISPRs as central elements used by bacteria to protect against foreign nucleic acids; however, emerging evidence points involvement in bacterial virulence. present study aimed identify the participation of one CRISPR-Cas protein, Cas3, virulence oral pathogen Porphyromonas gingivalis, an...
Summary Porphyromonas gingivalis is a Gram‐negative oral anaerobe associated with chronic adult periodontitis. Its ecological niche the gingival crevice, where organism adapts to challenges of infectious process such as host defence and bacterial products. Bacterial responses environmental changes are partly regulated by two‐component signal transduction systems. Several intact systems were annotated in genome P. , well an orphan regulator encoding homologue RprY, response from Bacteroides...
Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen which relies on highly adaptable metabolism to achieve broad pathogenesis. In one example of this flexibility, catalyze the NADH:quinone oxidoreductase step respiratory chain, P . has three different enzymes: NUO, NQR and NDH2, all carry out same redox function but have energy conservation ion transport properties. order better understand roles these enzymes, we constructed two series mutants: (i) single deletion mutants, each...
Abstract Aim To assess the effects of scaling and root planing (SRP) on dynamics gene expression by host microbiome in subgingival plaque samples. Materials Methods Fourteen periodontitis patients were closely monitored absence periodontal treatment for 12 months. During this period, comprehensive examination biofilm sample collection performed bi‐monthly. After months, clinical attachment level (CAL) data compiled analysed using linear mixed models (LMM) fitted to longitudinal CAL...
The ability of many human pathogens to infect requires their adhere the host surfaces as a first step in process.
There is a strong association between vitamin D levels and periodontal disease based on numerous epidemiological studies. We have previously shown that experimental deficiency of serum in mice leads to gingival inflammation alveolar bone loss. Treatment cultured oral epithelial cells with the active form D, 1,25(OH)
In this study, we characterized a serine protease from Tannerella forsythia that degrades gelatin, type I, and III collagen. is associated with periodontitis progression severity. The primary goal of research was to understand the mechanisms by which T. contributes progression. One our previous metatranscriptomic analysis revealed during highly expressed bfor_1659 ORF. N-terminal end homologous dipeptidyl aminopeptidase IV (DPP IV). DPP cleaves X-Pro or X-Ala dipeptide proteins. Collagen I...
ABSTRACT Imbalances of the microbiome also referred to as microbial dysbiosis, could lead a series different diseases. One factor that has been shown dysbiosis is exposure psychological stressors. Throughout evolution microorganisms human have developed systems for sensing host-associated signals such hormones associated with those stressors, enabling them recognize essential changes in its environment thus changing expression gene profile fit needs new environment. The most widely accepted...
Pseudomonas aeruginosa has four Na+/H+ antiporters that interconvert and balance Na+ H+ gradients across the membrane. These are important for bioenergetics ionic homeostasis. To understand these transporters, we constructed strains, each of which only one antiporter, i.e., NhaB, NhaP, NhaP2, Mrp. We also a quadruple deletion mutant no antiporters. Although P. have been studied previously, strains here present opportunity to characterize their kinetic properties in native membranes roles...