A5031808282- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Microbial Natural Products and Biosynthesis
- Antibiotic Resistance in Bacteria
- Geomagnetism and Paleomagnetism Studies
- Plant Micronutrient Interactions and Effects
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Microbial Fuel Cells and Bioremediation
- Genomics and Phylogenetic Studies
- Iron oxide chemistry and applications
- Antimicrobial Peptides and Activities
- Computational Drug Discovery Methods
- Bacteriophages and microbial interactions
Yale University
2024
Broad Institute
2024
Massachusetts Institute of Technology
2024
Massachusetts General Hospital
2024
University of Massachusetts Boston
2024
Rensselaer Polytechnic Institute
2021
SUMMARY The surge of antimicrobial resistance threatens efficacy current antibiotics, particularly against Pseudomonas aeruginosa , a highly resistant gram-negative pathogen. asymmetric outer membrane (OM) P. combined with its array efflux pumps provide barrier to xenobiotic accumulation, thus making antibiotic discovery challenging. We adapted PROSPECT 1 target-based, whole-cell screening strategy, discover small molecule probes that kill mutants depleted for essential proteins localized at...
The rising prevalence of antibiotic resistance threatens human health. While more sophisticated strategies for discovery are being developed, target elucidation new chemical entities remains challenging. In the postgenomic era, expression profiling can play an important role in mechanism-of-action (MOA) prediction by reporting on cellular response to perturbation. However, broad application transcriptomics has yet fulfill its promise transforming due challenges identifying most relevant,...
Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen which relies on highly adaptable metabolism to achieve broad pathogenesis. In one example of this flexibility, catalyze the NADH:quinone oxidoreductase step respiratory chain, P . has three different enzymes: NUO, NQR and NDH2, all carry out same redox function but have energy conservation ion transport properties. order better understand roles these enzymes, we constructed two series mutants: (i) single deletion mutants, each...
Abstract The rising prevalence of antibiotic resistance threatens human health. While more sophisticated strategies for discovery are being developed, target elucidation new chemical entities remains challenging. In the post-genomic era, expression profiling can play an important role in mechanism-of-action (MOA) prediction by reporting on cellular response to perturbation. However, broad application transcriptomics has yet fulfill its promise transforming due challenges identifying most...
Pseudomonas aeruginosa has four Na+/H+ antiporters that interconvert and balance Na+ H+ gradients across the membrane. These are important for bioenergetics ionic homeostasis. To understand these transporters, we constructed strains, each of which only one antiporter, i.e., NhaB, NhaP, NhaP2, Mrp. We also a quadruple deletion mutant no antiporters. Although P. have been studied previously, strains here present opportunity to characterize their kinetic properties in native membranes roles...