A5034154223- Bacterial biofilms and quorum sensing
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- Listeria monocytogenes in Food Safety
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Computational Drug Discovery Methods
- Seaweed-derived Bioactive Compounds
Broad Institute
2024
Massachusetts Institute of Technology
2024
Massachusetts General Hospital
2024
University of Massachusetts Boston
2024
SUMMARY The surge of antimicrobial resistance threatens efficacy current antibiotics, particularly against Pseudomonas aeruginosa , a highly resistant gram-negative pathogen. asymmetric outer membrane (OM) P. combined with its array efflux pumps provide barrier to xenobiotic accumulation, thus making antibiotic discovery challenging. We adapted PROSPECT 1 target-based, whole-cell screening strategy, discover small molecule probes that kill mutants depleted for essential proteins localized at...
The rising prevalence of antibiotic resistance threatens human health. While more sophisticated strategies for discovery are being developed, target elucidation new chemical entities remains challenging. In the postgenomic era, expression profiling can play an important role in mechanism-of-action (MOA) prediction by reporting on cellular response to perturbation. However, broad application transcriptomics has yet fulfill its promise transforming due challenges identifying most relevant,...
Crude cell wall (CCW), enzyme purified (PF), peptidoglycans (PEP), and teichoic acid fractions from two strains of Listeria monocytogenes (EGD a human isolate) were analyzed by electron microscopy, chemical analyses, various immunological assays. Electron microscopy analyses revealed that both quite similar. The CCW, PF, PEP mitogenic for mouse lymphocytes, could activate macrophages in vivo but not vitro, stimulated nonspecific immunity to Candida albicans. PF the CCW induced migration...
Abstract The rising prevalence of antibiotic resistance threatens human health. While more sophisticated strategies for discovery are being developed, target elucidation new chemical entities remains challenging. In the post-genomic era, expression profiling can play an important role in mechanism-of-action (MOA) prediction by reporting on cellular response to perturbation. However, broad application transcriptomics has yet fulfill its promise transforming due challenges identifying most...