A5029373488- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Amoebic Infections and Treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Neonatal and Maternal Infections
- Virology and Viral Diseases
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Trace Elements in Health
- Chemical Reactions and Isotopes
- Environmental DNA in Biodiversity Studies
- Immune Response and Inflammation
- Chemical Synthesis and Characterization
- Bacterial Genetics and Biotechnology
- Complement system in diseases
- Respiratory viral infections research
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Acoustic Wave Resonator Technologies
- Cytomegalovirus and herpesvirus research
- Child Nutrition and Water Access
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
Janssen (Netherlands)
2016-2021
Augusta University Health
2021
National Institute for Biological Standards and Control
2011-2015
University of Oxford
2007
University of Illinois Urbana-Champaign
1969
Fungi are the principal degraders of biomass in most terrestrial ecosystems. In contrast to surface environments, deep-sea environmental gene libraries have suggested that fungi rare and non-diverse high-pressure marine environments. Here, we report diversity from 11 samples around world representing depths 1,500 4,000 m (146-388 atm) two shallower water column (250 500m). We sequenced 239 clones 10 fungal-specific 18S rRNA constructed these samples, which detected only 18 fungal 18S-types...
ObjectivesOuter membrane vesicle (OMV) vaccines are used against outbreaks of capsular group B Neisseria meningitidis (MenB) caused by strains expressing particular PorA outer proteins (OMPs). Ferric enterobactin receptor (FetA) is another variable OMP that induces type-specific bactericidal antibodies, and the combination judiciously chosen FetA variants in vaccine formulations a potential approach to broaden protection such vaccines.MethodsThe OMV MenPF-1 was generated genetically...
FrpB is an outer membrane transporter from Neisseria meningitidis, the causative agent of meningococcal meningitis. It a member TonB-dependent (TBDT) family and responsible for iron uptake into periplasm. subject to high degree antigenic variation, principally through region hypervariable sequence exposed at cell surface. From crystal structures two variants, we identify bound ferric ion within structure which induces structural changes on binding are consistent with it being transported...
Group B Neisseria meningitidis, an endotoxin-producing Gram-negative bacterium, causes the highest incidence of group meningococcus (MenB) disease in first year life. The Bexsero vaccine is indicated Europe from 8 weeks age. Endotoxin components outer membrane vesicles (OMVs) or soluble lipopolysaccharide (LPS) represent a potential source inflammation and residual reactogenicity. purpose this study was to compare novel candidate MenB formulations with licensed vaccines, including Bexsero,...
Factor H binding protein (fHbp) is a major antigenic component of novel vaccines designed to protect against meningococcal disease. Prediction the potential coverage these difficult, as fHbp antigenically variable and levels expression differ among isolates. Transcriptional regulation gene poorly understood, although evidence suggests that oxygen availability involved. In this study iron accessibility was found affect transcription. However, differed clonal complexes (ccs). For majority...
Abstract Vaccines against Staphylococcus aureus have eluded researchers for >3 decades while the burden of staphylococcal diseases has increased. Early vaccine attempts mainly used rodents to characterize preclinical efficacy, and all subsequently failed in human clinical efficacy trials. More recently, leukocidin AB (LukAB) gained interest as a antigen. We developed minipig deep surgical wound infection model offering 3 independent readouts: bacterial load at superficial deep-seated...
Methyl esters of polyunsaturated fatty acids labeled with tritium were prepared by partial stereoselective reduction the corresponding acetylenic tritiated disiamylborane, followed protonolysis acetic acid. The labeling was strictly specific, and atoms located only at carbon forming unsaturated bond(s). Synthesis some acid methyl methylene-interrupted trans-cis double bonds is also reported.
Following the introduction of effective protein-polysaccharide conjugate vaccines against capsular group C meningococcal disease in Europe, meningococci B remain a major cause death and can result debilitating sequelae. The outer membrane proteins PorA FetA have previously been shown to induce bactericidal antibodies humans. Despite considerable antigenic variation among OMPs meningococci, systematic molecular epidemiological studies revealed this is highly structured so that limited...
Invasive meningococcal disease causes over 3500 cases each year in Europe, with particularly high incidence among young children. Among serogroup B meningococci, which cause most of the cases, diversity outer membrane proteins (OMPs) is observed endemic situations; however, comprehensive molecular epidemiological data are available for and distribution OMPs PorA FetA these can be used to rationally design a vaccine coverage case isolates. The aim this study was determine whether vesicles...